United States
Germany
Japan
United Kingdom
China
Idh3a (BC034273) Mouse Tagged ORF Clone
CAT＃: MG203919
- TrueORF®
Idh3a (tGFP-tagged) - Mouse isocitrate dehydrogenase 3 (NAD+) alpha (cDNA clone MGC:19310 IMAGE:4166193)
ORF Plasmid: DDK
"BC034273" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 2,850.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1110003P10Rik; 1500012E04Rik; AA407078; AI316514 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203919 representing BC034273
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCCCTCCAGAAGCCAAGGAGTCCATGGATAAGAACAAGATGGGCTTGAAAGGCCCACTAAAGACCC CAATAGCCGCTGGCCATCCATCTATGAATCTGTTGCTTCGTAAGACATTTGACCTTTATGCCAATGTCCG GCCATGTGTCTCAATTGAAGGTTATAAAACCCCTTACACGGATGTAAATATCGTCACCATCCGAGAGAAC ACGGAAGGAGAATACAGTGGAATTGAGCATGTGATCGTTGATGGGGTTGTGCAGAGCATCAAGCTCATCA CCGAAGAAGCAAGCAAGCGCATTGCAGAGTTTGCCTTCGAGTACGCTCGGAACAACCACCGGAGCAACGT CACAGCTGTGCACAAAGCTAACATCATGAGGATGTCAGATGGGCTCTTTCTGCAAAAATGCAGGGAAGTT GCGGAGAACTGTAAAGACATTAAATTTAACGAGATGTACCTTGATACTGTATGTTTAAATATGGTACAAG ACCCATCCCAGTTTGATGTTCTTGTCATGCCAAATTTATACGGAGACATCCTTAGTGATCTGTGTGCAGG ACTGATTGGAGGTCTTGGGGTGACTCCAAGTGGCAATATTGGAGCCAACGGTGTTGCCATCTTTGAATCG GTTCATGGAACAGCCCCGGACATTGCAGGCAAGGACATGGCCAACCCCACGGCCCTCCTGCTTAGTGCTG TGATGATGCTTCGCCACATGGGACTTTTTGACCATGCAGCAAAAATCGAGGCTGCATGTTTTGCTACAAT TAAGGATGGAAAGAGCTTAACAAAAGATCTGGGAGGCAACGCGAAGTGCTCTGACTTCACAGAAGAAATC TGTCGTAGAGTCAAAGACTTAGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203919 representing BC034273
Red=Cloning site Green=Tags(s) MIPPEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIREN TEGEYSGIEHVIVDGVVQSIKLITEEASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREV AENCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFES VHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAAKIEAACFATIKDGKSLTKDLGGNAKCSDFTEEI CRRVKDLD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC034273 |
| ORF Size | 866 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC034273, AAH34273 |
| RefSeq Size | 2416 bp |
| RefSeq ORF | 866 bp |
| Locus ID | 67834 |
| Gene Summary | Catalytic subunit of the enzyme which catalyzes the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR203919 | Idh3a (Myc-DDK-tagged) - Mouse isocitrate dehydrogenase 3 (NAD+) alpha (cDNA clone MGC:19310 IMAGE:4166193) |
CNY 2,400.00 |
|
| MR203919L3 | Lenti ORF clone of Idh3a (Myc-DDK-tagged) - Mouse isocitrate dehydrogenase 3 (NAD+) alpha (cDNA clone MGC:19310 IMAGE:4166193) |
CNY 4,750.00 |
|
| MR203919L4 | Lenti ORF clone of Idh3a (mGFP-tagged) - Mouse isocitrate dehydrogenase 3 (NAD+) alpha (cDNA clone MGC:19310 IMAGE:4166193) |
CNY 4,750.00 |
