Igfbp3 (BC058261) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	IGFBP-3, IGgfbp3
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG204018 representing BC058261 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATCCCGCGCGCCCCGCGCTCTGGGCGGCTGCGCTCACTGCCCTCACTCTGCTCCGCGGACCGCCGG TGGCGCGAGCTGGCGCGGGCGCGGTGGGCGCGGGCCCCGTGGTGCGCTGCGAACCGTGCGACGCGCGTGC GCTGTCCCAGTGCGCGCCTCCGCCCACTGCGCCCGCGTGCACGGAGCTGGTGCGAGAGCCCGGCTGCGGC TGCTGCCTGACTTGCGCGCTGCGCGAAGGCGACGCGTGCGGCGTCTACACGGAGCGCTGTGGCACCGGCC TCCGCTGCCAGCCGCGGCCCGCGGAGCAGTACCCGCTGAGGGCGCTGCTGAATGGCCGCGGGTTCTGCGC CAACGCCAGCGCTGCAGGCAGCCTAAGCACCTACCTCCCCTCCCAACCTGCTCCAGGAAACATCAGTGAG TCCGAGGAGGAGCACAATGCTGGGAGTGTGGAAAGCCAGGTTGTCCCCAGCACACACCGAGTGACCGATT CCAAGTTCCATCCACTCCATGCCAAGATGGATGTCATCAAAAAAGGCCACGCCAGGGACAGCCAGCGCTA CAAAGTTGACTATGAGTCTCAAAGCACAGACACCCAGAACTTCTCCTCCGAGTCTAAGCGGGAGACAGAA TACGGTCCCTGCCGCAGAGAAATGGAGGACACACTGAATCATCTGAAGTTCCTCAATGTGCTGAGTCCCA GAGGCGTCCACATCCCAAACTGTGACAAGAAGGGGTTCTATAAGAAGAAGCAGTGCCGCCCTTCCAAAGG CAGAAAGCGGGGCTTCTGCTGGTGTGTGGACAAGTATGGGCAGCCCTTGCCAGGCTACGACACCAAGGGG AAAGACGACGTACATTGCCTCAGCGTGCAGAGCCAG ACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG204018 representing BC058261 Red=Cloning site Green=Tags(s) MHPARPALWAAALTALTLLRGPPVARAGAGAVGAGPVVRCEPCDARALSQCAPPPTAPACTELVREPGCG CCLTCALREGDACGVYTERCGTGLRCQPRPAEQYPLRALLNGRGFCANASAAGSLSTYLPSQPAPGNISE SEEEHNAGSVESQVVPSTHRVTDSKFHPLHAKMDVIKKGHARDSQRYKVDYESQSTDTQNFSSESKRETE YGPCRREMEDTLNHLKFLNVLSPRGVHIPNCDKKGFYKKKQCRPSKGRKRGFCWCVDKYGQPLPGYDTKG KDDVHCLSVQSQ TRPLE - GFP Tag - V
Restriction Sites	SgfI-NotI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC058261
ORF Size	878 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC058261, AAH58261
RefSeq Size	2432 bp
RefSeq ORF	878 bp
Locus ID	16009
Gene Summary	IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.[UniProtKB/Swiss-Prot Function]

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