Rnps1 (BC002061) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG204288 representing BC002061 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTATCAGGAGTGAAAAAGAAGAGCTTGCTAGGAGTCAAAGAGAATAATAAAAAGTCCAGCACTA GGGCTCCTTCTCCTACCAAACGCAAGGACCGATCTGATGAGAAGTCCAAGGATCGATCTAAAGATAAAGG GGCCACTAAAGAGTCAAGTGAGAAGGATCGTGGCAGAGATAAGACTCGGAAGAGACGCAGTGCTTCAAGC GGAAGCAGCAGTACCAGGTCTAGGTCCAGCTCAACCTCCAGCTCGGGCTCCAGCACCAGCACAGGCTCAA GCAGTGGCTCCAGCTCGTCCTCTGCATCCAGCCGCTCAGGAAGCTCCAGCACGTCCCGGAGCTCCAGTTC TAGCAGCTCCTCCGGCTCCCCAAGCCCTTCTCGGCGCAGGCATGACAACAGGCGGCGCTCCCGCTCCAAA TCCAAACCACCTAAAAGAGATGAAAAAGAGAGGAAAAGGCGGAGCCCTTCACCTAAACCAACCAAAGTGC ACATTGGGAGGCTCACCAGGAATGTGACCAAGGATCATATCATGGAAATATTTTCTACTTACGGGAAAAT CAAAATGATTGACATGCCTGTCGAGAGGATGCATCCTCACCTCTCCAAAGGCTATGCATATGTGGAGTTT GAGAATCCCGATGAAGCAGAGAAGGCTCTGAAACACATGGATGGAGGACAAATTGATGGCCAAGAGATCA CTGCTACTGCTGTGTTGGCACCCTGGCCTCGGCCACCACCTCGGCGATTCAGCCCTCCTAGGAGGATGCT TCCACCACCTCCCATGTGGCGTAGGTCACCCCCACGGATGAGGAGAAGGTCTCGATCCCCAAGACGCAGG TCCCCTGTGCGTAGGAGGTCTCGCTCTCCTGGCCGCCGCCGCCACAGGAGCCGATCCAGCTCCAACTCCT CCCGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG204288 representing BC002061 Red=Cloning site Green=Tags(s) MDLSGVKKKSLLGVKENNKKSSTRAPSPTKRKDRSDEKSKDRSKDKGATKESSEKDRGRDKTRKRRSASS GSSSTRSRSSSTSSSGSSTSTGSSSGSSSSSASSRSGSSSTSRSSSSSSSSGSPSPSRRRHDNRRRSRSK SKPPKRDEKERKRRSPSPKPTKVHIGRLTRNVTKDHIMEIFSTYGKIKMIDMPVERMHPHLSKGYAYVEF ENPDEAEKALKHMDGGQIDGQEITATAVLAPWPRPPPRRFSPPRRMLPPPPMWRRSPPRMRRRSRSPRRR SPVRRRSRSPGRRRHRSRSSSNSSR TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC002061
ORF Size	915 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC002061, AAH02061
RefSeq Size	1872 bp
RefSeq ORF	917 bp
Locus ID	19826
Gene Summary	Part of pre- and post-splicing multiprotein mRNP complexes. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP and PSAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. The ASAP complex can inhibit RNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Enhances the formation of the ATP-dependent A complex of the spliceosome. Involved in both constitutive splicing and, in association with SRP54 and TRA2B/SFRS10, in distinctive modulation of alternative splicing in a substrate-dependent manner. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function. Participates in mRNA 3'-end cleavage. Involved in UPF2-dependent nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Also mediates increase of mRNA abundance and translational efficiency. Binds spliced mRNA 20-25 nt upstream of exon-exon junctions (By similarity).[UniProtKB/Swiss-Prot Function]

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