Ggh (NM_010281) Mouse Tagged ORF Clone
CAT#: MG204547
- TrueORF®
Ggh (tGFP-tagged) - Mouse gamma-glutamyl hydrolase (Ggh)
ORF Plasmid: DDK
"NM_010281" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | gamma-GH |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG204547 representing NM_010281
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAACCTGGGCTACCTGCTGTGCTTGTTGGGTTTGCTACTATGCGGGCTTTCAAGTCCCGGGATGT CCAGACCCTACAACCATGGCTCCGAGAGGCCCATCATCGGAGTAGTAATGCAAGAATGTTTTGGGAAAAT GGCGAAGTTGGGAAACTACTACATTGCTGCATCCTATGTGAAGTATATAGAGTCTGCAGGTGCACGAGTT GTGCCCATAAGGCCTGATCTTAGTGATGCAGAGTATGAAGAACTTTTTAGATCTATTAATGGAGTTCTCC TTCCTGGAGGAGGTGCTAACCTAACAGATTCAGGTTATTCCCGAGTGGCCAAAATATTTTTCAGCAAGGC TCTAGAGAGCTTTGATAATGGAGGCCATTTTCCTGTGTGGGGAACATGCCTTGGATTTGAGGAGCTTTCT GTCCTGGTTAGTGGAGAGAACTTACTAACGAGTACAGACACTAAGTCAAAGAAATTGCCCTTGAACTTCA CTGAAGGTGCAAGGAAGAGCAGAATGTTCAAACATTTTCCTACTGAGTTATTGGATTCATTAGCACTAGA AAATCTGACCGCAAATTTTCACAAATGGAGCCTCTCTGTGAAGAATTTTACAGAAAATGAAAAGTTAAAG AAGTTTTTCAATATATTAACAACCAATACAGATGGCAAAACTGAGTTCATTTCATCCATGGAAGGATTTA AGTATCCAGTGTATGCTGTCCAGTGGCATCCTGAGAAAGCACCATTTGAGTGGAAGAATCTGGGCGGCAT TTCTCATGCACCAAATGCTGTGAAGACTTCATTCTACTTAGCAGAATTTTTAGTTTCTGAAGCTCGGAAG AACAGTCACCACTTTGAGAACGTGGTTAAGGAGACGGCATCCTTGATTTACAAGGTCTATCCCATTTATA CTGGAAATATTTCTTCATTTCAGCAAGCTTATATGTTTGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204547 representing NM_010281
Red=Cloning site Green=Tags(s) MANLGYLLCLLGLLLCGLSSPGMSRPYNHGSERPIIGVVMQECFGKMAKLGNYYIAASYVKYIESAGARV VPIRPDLSDAEYEELFRSINGVLLPGGGANLTDSGYSRVAKIFFSKALESFDNGGHFPVWGTCLGFEELS VLVSGENLLTSTDTKSKKLPLNFTEGARKSRMFKHFPTELLDSLALENLTANFHKWSLSVKNFTENEKLK KFFNILTTNTDGKTEFISSMEGFKYPVYAVQWHPEKAPFEWKNLGGISHAPNAVKTSFYLAEFLVSEARK NSHHFENVVKETASLIYKVYPIYTGNISSFQQAYMFD TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010281 |
ORF Size | 951 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010281.2, NP_034411.2 |
RefSeq Size | 1340 bp |
RefSeq ORF | 954 bp |
Locus ID | 14590 |
UniProt ID | Q9Z0L8 |
Gene Summary | Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. Acts as endopeptidase. Lysosomal enzyme is activated by sulfhydryl compounds.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200869 | Ggh (untagged) - Mouse gamma-glutamyl hydrolase (Ggh), (10ug) |
CNY 2,000.00 |
|
MR204547 | Ggh (Myc-DDK-tagged) - Mouse gamma-glutamyl hydrolase (Ggh) |
CNY 2,081.00 |
|
MR204547L3 | Lenti ORF clone of Ggh (Myc-DDK-tagged) - Mouse gamma-glutamyl hydrolase (Ggh) |
CNY 4,750.00 |
|
MR204547L4 | Lenti ORF clone of Ggh (mGFP-tagged) - Mouse gamma-glutamyl hydrolase (Ggh) |
CNY 4,750.00 |