Plscr1 (BC002017) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	NOR1, TRA1, MmTRA1a, MmTRA1b, MuPLSCR2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG204796 representing BC002017 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAACCACAGCAAGCAAACTGAGGCTCCCCACCCGGGAACATATATGCCAGCTGGGTATCCCCCTC CGTATCCACCAGCAGCTTTCCAAGGACCTTCAGACCATGCTGCTTACCCCATACCCCAGGCTGGCTACCA AGGGCCTCCGGGCCCCTATCCAGGGCCCCAACCTGGCTACCCAGTCCCACCAGGAGGTTATGCAGGTGGT GGCCCTAGTGGCTTTCCTGTCCAAAATCAGCCAGCATATAATCATCCAGGTGGGCCTGGGGGGACCCCAT GGATGCCAGCCCCCCCACCTCCACTGAACTGTCCACCGGGGCTGGAATACTTAGCTCAGATTGATCAGCT TCTGGTTCATCAGCAAATTGAGCTTCTGGAAGTCTTAACAGGCTTTGAAACAAATAACAAATATGAAATC AAGAACAGCCTCGGGCAGAGAGTTTACTTTGCAGTGGAAGATACTGACTGCTGTACCCGAAACTGCTGTG GGGCGTCTAGACCTTTCACCTTGAGGATCCTGGATAATCTGGGCCGAGAAGTCATGACTCTGGAGCGACC TCTGAGATGCAGTAGCTGCTGCTTCCCCTGCTGCCTCCAGGAGATAGAAATCCAGGCTCCTCCTGGGGTG CCAGTAGGTTATGTGACTCAGACCTGGCACCCATGTCTGCCCAAGTTCACTCTCCAAAATGAGAAGAAGC AGGATGTCCTGAAAGTAGTTGGTCCGTGTGTTGTGTGTAGCTGCTGTTCCGACATTGACTTTGAGCTCAA ATCTCTAGATGAAGAATCAGTAGTTGGCAAAATTTCTAAGCAGTGGTCTGGTTTTGTGAGAGAGGCCTTC ACGGATGCAGACAACTTTGGGATCCAGTTCCCGCTAGACCTGGATGTGAAGATGAAAGCTGTGATGCTTG GTGCTTGTTTCCTCATAGATTTCATGTTTTTTGAAAGAACTGGAAACGAGGAACAAAGATCAGGAGTATG G ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG204796 representing BC002017 Red=Cloning site Green=Tags(s) MENHSKQTEAPHPGTYMPAGYPPPYPPAAFQGPSDHAAYPIPQAGYQGPPGPYPGPQPGYPVPPGGYAGG GPSGFPVQNQPAYNHPGGPGGTPWMPAPPPPLNCPPGLEYLAQIDQLLVHQQIELLEVLTGFETNNKYEI KNSLGQRVYFAVEDTDCCTRNCCGASRPFTLRILDNLGREVMTLERPLRCSSCCFPCCLQEIEIQAPPGV PVGYVTQTWHPCLPKFTLQNEKKQDVLKVVGPCVVCSCCSDIDFELKSLDEESVVGKISKQWSGFVREAF TDADNFGIQFPLDLDVKMKAVMLGACFLIDFMFFERTGNEEQRSGVW TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC002017
ORF Size	983 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC002017, AAH02017
RefSeq Size	1499 bp
RefSeq ORF	983 bp
Locus ID	22038
Gene Summary	May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.[UniProtKB/Swiss-Prot Function]

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