Aptx (BC021872) Mouse Tagged ORF Clone

CAT#: MG204974

  • TrueORF®

Aptx (tGFP-tagged) - Mouse aprataxin (cDNA clone MGC:28249 IMAGE:3994375)



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Mouse Protein / Lysate Request

CNY 3,140.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 2410016G21Rik; AA388047; FHA-HIT
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG204974 representing BC021872
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGAGTGTGCTGGTTGGTGAGACAGGACAGCAGGCACCAGCGAATCAAACTTCCCCATTTGGAAGCTG
TTGTGATTGGTCGAAGCCCAGAGACCAAGATCACAGATAAGAAATGTTCCCGACAGCAAGTACAGTTGAA
AGCAGAGTGTAACAAGGGATATGTCAAAGTACAGCAGATGGGGGTCAACCCCACCAGCATTGACTCGGGC
GTCATCGGGAAGGACCAAGAGAAGCTGCTGCTGCCTGGTCAGGTTCTCCACATGGTGAATGGACTTTATC
CATACATCGTAGAGTTTGAGGAAGTGGCAGAGAGCCCTAACCTAACACAGAGGAAGAGAAAGAGGTCAGA
CTGTGATAGTGAGGAGATGGAAGCTGAGTCTGGGACAGGGCTGGCACCTGGGAGCAGCCCCAGCCAGTGC
TCTGTGTCCCCTAAGAAGGACAAGAATGGAGCCACCAAAAAGGAATCACTGGGCCACTGGAGTCAAGGCT
TGAAGATGTCTATGAAAGACCCCAAAATGCAGGTTTACAAAGACGACCAGGTGGTGGTGATTAAGGATAA
ATACCCCAAGGCCCGTCACCACTGGCTGGTCTTACCGTGGGCCTCCATTTCCAGTCTGAAGGTTGTGACC
AGTGAACACCTTGAACTTCTCAAACATATGCACGCTGTGGGGGAGAAGGTGATAGCAGAGTTTGCTGGAT
CCAGCAAACTGCGCTTCCGATTGGGCTACCATGCCATTCCCAGCATGAGCCACGTACATCTTCATGTGAT
CAGCCAGGATTTTGATTCTCCTTGCCTTAAAAACAAAAAGCATTGGAATTCTTTTAATACAGAATACTTT
CTGGAATCACAAGCTGTGATCAAGATGGTTCAGGAAGCCGGCAGAGTGACTGTTAAAGATGGCACTTGTG
AGCTCTTGAAGCTGCCTCTCCGTTGCCATGAGTGTCAGCAGCTGCTGCCTTCCATCCCGCAGCTGAAAGA
GCACCTCAGGAAGCACTGGGGCGGG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG204974 representing BC021872
Red=Cloning site Green=Tags(s)

MRVCWLVRQDSRHQRIKLPHLEAVVIGRSPETKITDKKCSRQQVQLKAECNKGYVKVQQMGVNPTSIDSG
VIGKDQEKLLLPGQVLHMVNGLYPYIVEFEEVAESPNLTQRKRKRSDCDSEEMEAESGTGLAPGSSPSQC
SVSPKKDKNGATKKESLGHWSQGLKMSMKDPKMQVYKDDQVVVIKDKYPKARHHWLVLPWASISSLKVVT
SEHLELLKHMHAVGEKVIAEFAGSSKLRFRLGYHAIPSMSHVHLHVISQDFDSPCLKNKKHWNSFNTEYF
LESQAVIKMVQEAGRVTVKDGTCELLKLPLRCHECQQLLPSIPQLKEHLRKHWGG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC021872
ORF Size 1007 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC021872, AAH21872
RefSeq Size 1407 bp
RefSeq ORF 1007 bp
Locus ID 66408
Gene Summary DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined (PubMed:16964241). Also able to hydrolyze adenosine 5'-monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity (By similarity). Likewise, catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA, but has higher specific activity with 5'-linked adenosine (AppDNA) (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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