United States
Germany
Japan
United Kingdom
China
Wnt7a (NM_009527) Mouse Tagged ORF Clone
CAT＃: MG205285
- TrueORF®
Wnt7a (tGFP-tagged) - Mouse wingless-related MMTV integration site 7A (Wnt7a)
ORF Plasmid: DDK
"NM_009527" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7,088.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI849442; px; tw; Wnt-7a |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205285 representing NM_009527
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCGGAAAGCGCGGCGCTGCCTGGGCCACCTCTTTCTCAGCCTGGGCATAGTCTACCTCCGGATCG GTGGCTTCTCTTCGGTGGTAGCTCTGGGTGCGAGCATCATCTGTAACAAGATCCCAGGCCTGGCTCCCAG ACAGCGGGCAATCTGCCAGAGCCGGCCGGACGCCATCATCGTCATAGGAGAAGGCTCCCAAATGGGCCTG GACGAGTGTCAGTTTCAGTTCCGAAATGGCCGTTGGAACTGCTCAGCGCTGGGAGAGCGTACTGTCTTCG GGAAGGAGCTCAAAGTGGGGAGTCGGGAGGCTGCCTTCACCTATGCGATTATCGCTGCGGGCGTGGCCCA TGCCATCACTGCTGCCTGCACCCAGGGCAACCTGAGCGACTGTGGCTGCGACAAGGAGAAGCAAGGCCAG TACCACCGGGACGAGGGCTGGAAGTGGGGTGGCTGCTCTGCCGACATCCGCTACGGCATCGGCTTCGCCA AGGTCTTCGTGGATGCCCGGGAGATCAAGCAGAATGCCCGGACGCTCATGAACTTACACAATAACGAGGC GGGTCGGAAGATCCTGGAGGAGAACATGAAGCTGGAGTGTAAGTGCCATGGTGTGTCAGGCTCCTGTACC ACTAAGACGTGCTGGACCACACTGCCACAGTTCCGAGAGCTAGGCTACGTGCTCAAGGACAAATACAACG AGGCCGTCCACGTGGAGCCTGTGCGTGCCAGTCGAAACAAGCGGCCCACCTTTCTGAAGATCAAGAAGCC CCTGTCCTACCGCAAGCCCATGGACACTGACCTGGTGTATATCGAGAAGTCACCCAATTACTGTGAAGAG GACCCAGTGACAGGCAGCGTGGGTACCCAGGGCCGAGCCTGCAATAAGACAGCCCCTCAGGCCAGTGGCT GTGACCTCATGTGCTGTGGCCGTGGCTACAACACACACCAGTACGCCCGGGTGTGGCAGTGCAACTGCAA ATTCCACTGGTGCTGCTACGTCAAGTGTAACACGTGCAGCGAGCGCACGGAGATGTATACGTGCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205285 representing NM_009527
Red=Cloning site Green=Tags(s) MTRKARRCLGHLFLSLGIVYLRIGGFSSVVALGASIICNKIPGLAPRQRAICQSRPDAIIVIGEGSQMGL DECQFQFRNGRWNCSALGERTVFGKELKVGSREAAFTYAIIAAGVAHAITAACTQGNLSDCGCDKEKQGQ YHRDEGWKWGGCSADIRYGIGFAKVFVDAREIKQNARTLMNLHNNEAGRKILEENMKLECKCHGVSGSCT TKTCWTTLPQFRELGYVLKDKYNEAVHVEPVRASRNKRPTFLKIKKPLSYRKPMDTDLVYIEKSPNYCEE DPVTGSVGTQGRACNKTAPQASGCDLMCCGRGYNTHQYARVWQCNCKFHWCCYVKCNTCSERTEMYTCK TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009527 |
| ORF Size | 1047 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009527.4 |
| RefSeq Size | 3176 bp |
| RefSeq ORF | 1050 bp |
| Locus ID | 22421 |
| UniProt ID | P24383 |
| Gene Summary | Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:18230341, PubMed:20530549, PubMed:23629626). Plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development (PubMed:7885472, PubMed:9769174, PubMed:9790192). Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed:28803732). Required for normal, sexually dimorphic development of the Mullerian ducts, and for normal fertility in both sexes (PubMed:9790192). Required for normal neural stem cell proliferation in the hippocampus dentate gyrus (PubMed:23629626). Required for normal progress through the cell cycle in neural progenitor cells, for self-renewal of neural stem cells, and for normal neuronal differentiation and maturation (PubMed:23629626). Promotes formation of synapses via its interaction with FZD5 (PubMed:20530549).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209655 | Wnt7a (untagged) - Mouse wingless-related MMTV integration site 7A (Wnt7a), (10ug) |
CNY 5,488.00 |
|
| MR205285 | Wnt7a (Myc-DDK-tagged) - Mouse wingless-related MMTV integration site 7A (Wnt7a) |
CNY 5,488.00 |
|
| MR205285L3 | Lenti ORF clone of Wnt7a (Myc-DDK-tagged) - Mouse wingless-related MMTV integration site 7A (Wnt7a) |
CNY 5,890.00 |
|
| MR205285L4 | Lenti ORF clone of Wnt7a (mGFP-tagged) - Mouse wingless-related MMTV integration site 7A (Wnt7a) |
CNY 5,890.00 |
