Homer3 (BC005773) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AW146114
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205426 representing BC005773 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCACAGCCAGGGAACAGCCAATCTTCAGCACCCGGGCGCACGTATTCCAGATCGACCCCACTACAA AGCGGAACTGGATCCCCGCCGGCAAGCACGCACTTACCGTGTCCTATTTCTATGATGCAACCCGAAATGT GTACCGCATCATCAGCATCGGGGGTGCCAAGGCCATCATCAACAGCACTGTCACTCCCAACATGACCTTC ACCAAAACCTCTCAGAAGTTCGGGCAATGGGCAGACAGTCGAGCCAACACTGTCTACGGCCTAGGCTTTG CCTCTGAACAGCAGCTGACCCAGTTTGCTGAGAAGTTTCAGGAGGTGAAAGAAGCTGCCAGGCTGGCTCG AGAGAAATCTCAAGATGGTGGAGAATTCACTAGTACTGGCCTGGCCCTTGCCTCCCATCAGGTTCCTCCA AGCCCCTTGGTCAGCACCAATGGTCCAGGCGAGGAAAAGCTGTTCCGTAGCCAGAGTGCGGACACCCCTG GCCCCACCGAGCGGGAACGGTTGAAGAAGATGCTGTCAGAAGGCTCTGTAGGGGAGGTCCAGTGGGAGGC AGAGTTCTTCGCGCTTCAGGACAGCAACCAGAGGTTGGCGGGAGCCCTTCGGGAGGCCAACGCAGCGGCC ACTCAGTGGAGGCAACAACTGGAGGTCCAACGTGCAGAGGCTGAACTCTTGCGGCAGCGGGTAGCAGAGC TGGAGGCCCAGGTGGCTGTAGAGCCAGTCCGGGCAGGAGAGAAAGAAGCAACCAGCCAGTCGGTGGAGCA GCTGGAGGCTCGGGTGCAGACCAAGGACCAGACTTTGAAGAATCAGAGCACTGGCACCCGAGAGGCTCCA GACACTGCCGAGCGCGAAGAGACACAGCAGCAAGTTCAGGACCTGGAGACCCGGAATGCAGAGCTGGAGC AGCAGCTGCGGGCGATGGAGTGCAACCTGGAGGAGGCGCGGGCCGAGCGGGAGCGCGCACGGGCGGAGGT GGGCCGGGCTGCGCAGCTGCTGGATGTTCGGCTGTTTGAGCTCAGCGAGCTGCGTGAAGGCCTGGCACGC CTGGCAGAGGCAGCACCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205426 representing BC005773 Red=Cloning site Green=Tags(s) MSTAREQPIFSTRAHVFQIDPTTKRNWIPAGKHALTVSYFYDATRNVYRIISIGGAKAIINSTVTPNMTF TKTSQKFGQWADSRANTVYGLGFASEQQLTQFAEKFQEVKEAARLAREKSQDGGEFTSTGLALASHQVPP SPLVSTNGPGEEKLFRSQSADTPGPTERERLKKMLSEGSVGEVQWEAEFFALQDSNQRLAGALREANAAA TQWRQQLEVQRAEAELLRQRVAELEAQVAVEPVRAGEKEATSQSVEQLEARVQTKDQTLKNQSTGTREAP DTAEREETQQQVQDLETRNAELEQQLRAMECNLEEARAERERARAEVGRAAQLLDVRLFELSELREGLAR LAEAAP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC005773
ORF Size	1070 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC005773, AAH05773
RefSeq Size	2393 bp
RefSeq ORF	1070 bp
Locus ID	26558
Gene Summary	Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses (By similarity). Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA (PubMed:18218901).[UniProtKB/Swiss-Prot Function]

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