3110048E14Rik (BC066174) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG205464 representing BC066174 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCAGTGGAAAAGACCACGAACAGAAGTGAGCAGAAATGCAGAAAGTTTTTAAAGAGCCTCATTA GAAAGCAACCCCAGGACCTGCTCTTGGTCATTGGGACTGGCGTGAGCGCAGCTGTGGCCCCAGGCATCCG AGCGCTCTGCTCTTGGAGGAGCTGCATTGAGGCGGTCATCGAGGCTGCGGAGCAGCTGGAGGTTCTGCAC CCTGGCGACGTTGCTGAGTTCCGGAGGAAGGTGATGAAGGATCGGGACCTGCTGGTTGTGGCCCATGATC TGATCCGGAAGATGTCACCTCGCACAGGCGACACCAAGCCCAACTTCTTCCAGGATTGCCTGATGGAGGT GTTTGACAGCTTGGAGCAGCACATTCAGAACCCGGTGGTGCTGCGGTCCATCCTCAGCCTGATGGACAGG GGCACCATGGTGCTGACCACCAACTACGACAACCTGCTGGAGATCTTCGGGCAGCAGCAGAGCAAGCCCA TGGAGTCCTTGGACTTGAAGGATAAGACGAAGGTTCTTCAGTGGGCAAGAGGCCATATTAAGTATGGAGT TCTTCATATTCATGGTTTGTACACGGACCCCTGTGGGATGGTGCTGGATCCATCGGGCTATAAAGATGTC ACTCAAGATCCGGAAGTCATGGAAGTCCTGCAAAATCTCTACCGAACCAAGTCATTCCTGTTCGTGGGCT GTGGCGAGACACTTCGGGACCAGATATTCCAGGCGCTCTTCTTGTACTCCGTGCCAAATAAGGTGGACTT GGAGCACTACATGGTAGTGCTCAAGGAAAACGAAGACCATTTCTTTAAGCATCAGGCAGATATGCTTTTG CATGGAATTAAAGTGGTGTCCTACGGGGACTGTTTTGACCTTTTCCCGGGATATGTGCAAGACCTTGCCA CTCAGATCTGCAAGCAGCGAAGTCCAGATGCTGAGCGAGTGGACAGCACCACCTTACTGGGTAATGCATG TCAGGACTGTGCAAAGAGGAAGTTGGAAGAGAATGGAATTGAAGTCACAAAAAAAGTTAGACAGTCAGAT ACTGATGATGCTGGAGGGTCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG205464 representing BC066174 Red=Cloning site Green=Tags(s) MESVEKTTNRSEQKCRKFLKSLIRKQPQDLLLVIGTGVSAAVAPGIRALCSWRSCIEAVIEAAEQLEVLH PGDVAEFRRKVMKDRDLLVVAHDLIRKMSPRTGDTKPNFFQDCLMEVFDSLEQHIQNPVVLRSILSLMDR GTMVLTTNYDNLLEIFGQQQSKPMESLDLKDKTKVLQWARGHIKYGVLHIHGLYTDPCGMVLDPSGYKDV TQDPEVMEVLQNLYRTKSFLFVGCGETLRDQIFQALFLYSVPNKVDLEHYMVVLKENEDHFFKHQADMLL HGIKVVSYGDCFDLFPGYVQDLATQICKQRSPDAERVDSTTLLGNACQDCAKRKLEENGIEVTKKVRQSD TDDAGGS TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC066174
ORF Size	1073 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC066174, AAH66174
RefSeq Size	2511 bp
RefSeq ORF	1073 bp
Locus ID	73225

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