3110048E14Rik (BC066174) Mouse Tagged ORF Clone
CAT#: MG205464
- TrueORF®
3110048E14Rik (tGFP-tagged) - Mouse RIKEN cDNA 3110048E14 gene (cDNA clone MGC:76568 IMAGE:30109713)
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CNY 3,140.00
货期*
4周
规格
经常一起买 (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG205464 representing BC066174
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCAGTGGAAAAGACCACGAACAGAAGTGAGCAGAAATGCAGAAAGTTTTTAAAGAGCCTCATTA GAAAGCAACCCCAGGACCTGCTCTTGGTCATTGGGACTGGCGTGAGCGCAGCTGTGGCCCCAGGCATCCG AGCGCTCTGCTCTTGGAGGAGCTGCATTGAGGCGGTCATCGAGGCTGCGGAGCAGCTGGAGGTTCTGCAC CCTGGCGACGTTGCTGAGTTCCGGAGGAAGGTGATGAAGGATCGGGACCTGCTGGTTGTGGCCCATGATC TGATCCGGAAGATGTCACCTCGCACAGGCGACACCAAGCCCAACTTCTTCCAGGATTGCCTGATGGAGGT GTTTGACAGCTTGGAGCAGCACATTCAGAACCCGGTGGTGCTGCGGTCCATCCTCAGCCTGATGGACAGG GGCACCATGGTGCTGACCACCAACTACGACAACCTGCTGGAGATCTTCGGGCAGCAGCAGAGCAAGCCCA TGGAGTCCTTGGACTTGAAGGATAAGACGAAGGTTCTTCAGTGGGCAAGAGGCCATATTAAGTATGGAGT TCTTCATATTCATGGTTTGTACACGGACCCCTGTGGGATGGTGCTGGATCCATCGGGCTATAAAGATGTC ACTCAAGATCCGGAAGTCATGGAAGTCCTGCAAAATCTCTACCGAACCAAGTCATTCCTGTTCGTGGGCT GTGGCGAGACACTTCGGGACCAGATATTCCAGGCGCTCTTCTTGTACTCCGTGCCAAATAAGGTGGACTT GGAGCACTACATGGTAGTGCTCAAGGAAAACGAAGACCATTTCTTTAAGCATCAGGCAGATATGCTTTTG CATGGAATTAAAGTGGTGTCCTACGGGGACTGTTTTGACCTTTTCCCGGGATATGTGCAAGACCTTGCCA CTCAGATCTGCAAGCAGCGAAGTCCAGATGCTGAGCGAGTGGACAGCACCACCTTACTGGGTAATGCATG TCAGGACTGTGCAAAGAGGAAGTTGGAAGAGAATGGAATTGAAGTCACAAAAAAAGTTAGACAGTCAGAT ACTGATGATGCTGGAGGGTCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205464 representing BC066174
Red=Cloning site Green=Tags(s) MESVEKTTNRSEQKCRKFLKSLIRKQPQDLLLVIGTGVSAAVAPGIRALCSWRSCIEAVIEAAEQLEVLH PGDVAEFRRKVMKDRDLLVVAHDLIRKMSPRTGDTKPNFFQDCLMEVFDSLEQHIQNPVVLRSILSLMDR GTMVLTTNYDNLLEIFGQQQSKPMESLDLKDKTKVLQWARGHIKYGVLHIHGLYTDPCGMVLDPSGYKDV TQDPEVMEVLQNLYRTKSFLFVGCGETLRDQIFQALFLYSVPNKVDLEHYMVVLKENEDHFFKHQADMLL HGIKVVSYGDCFDLFPGYVQDLATQICKQRSPDAERVDSTTLLGNACQDCAKRKLEENGIEVTKKVRQSD TDDAGGS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC066174 |
ORF Size | 1073 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC066174, AAH66174 |
RefSeq Size | 2511 bp |
RefSeq ORF | 1073 bp |
Locus ID | 73225 |
Documents
Product Manuals |
FAQs |
SDS |
Resources
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