Mapk13 (BC001992) Mouse Tagged ORF Clone

CAT#: MG205630

  • TrueORF®

Mapk13 (tGFP-tagged) - Mouse mitogen activated protein kinase 13 (cDNA clone MGC:5725 IMAGE:3492903)



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Mouse Protein / Lysate Request

CNY 3,140.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms SAPK4; Serk4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG205630 representing BC001992
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCCTCACTCGGAAAAGGGGCTTCTACAAGCAGGACATCAACAAGACTGCCTGGGAGCTACCCAAGA
CCTACCTGGCGCCCGCGCACGTCGGCAGCGGGGCCTATGGAGCGGTGTGCTCGGCCATCGACAAGCGGAC
AGGGGAGAAGGTGGCCATCAAGAAGCTGAGCCGGCCCTTCCAGTCGGAGATCTTTGCCAAGCGCGCTTAC
CGCGAGCTTCTGCTCTTGAAGCACATGCACCATGAGAACGTCATTGGGCTTCTGGATGTCTTCACCCCTG
CGTCTTCCCTTCGGAGCTTCCATGATTTCTACCTGGTGATGCCCTTCATGCAGACAGACCTACAGAAGAT
CATGGGGATGGAATTCAGCGAGGATAAGGTCCAGTACTTGGTGTACCAGATGCTCAAAGGTCTAAAGTAC
ATCCACTCCGCTGGCATCGTCCACAGGGACCTGAAACCGGGCAACCTGGCTGTGAATGAAGACTGTGAGC
TGAAGATCCTGGACTTTGGGCTGGCCCGCCACACAGACACTGAGATGACGGGCTATGTGGTGACCCGCTG
GTACCGGGCCCCCGAGGTGATCCTCAGCTGGATGCATTACAACCAGACAGTCGACATCTGGTCTGTTGGT
TGCATCATGGCAGAAATGCTGACTGGAAAGACACTCTTCAAGGGCAAGGACTACCTGGACCAGCTGACCC
AGATCCTGAAAGTGACTGGGGTGCCAGGTGCCGAGTTCGTGCAGAAGCTGAAAGACAAGGCGGCCAAATC
CTATATTCAGTCCCTGCCCCAGAGCCCCAAGAAGGATTTCACACAGCTCTTTCCACGCGCCAGTCCGCAA
GCTGCAGACCTGCTCGACAAGATGCTGGAGCTGGATGTGGACAAGCGTCTGACCGCTGCTCAGGCACTGG
CTCACCCCTTCTTTGAACCCTTCCGGGACCCTGAGGAGGAGACAGAGGCCCAGCAGCCTTTTGATGATGC
CTTAGAACATGAGAAACTCAGTGTGGACGAATGGAAACAACACATCTACAAAGAGATCTCAAACTTCAGT
CCCATAGCCCGGAAGGACTCACGGCGACGAAGTGGCATGAAGCTGCAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG205630 representing BC001992
Red=Cloning site Green=Tags(s)

MSLTRKRGFYKQDINKTAWELPKTYLAPAHVGSGAYGAVCSAIDKRTGEKVAIKKLSRPFQSEIFAKRAY
RELLLLKHMHHENVIGLLDVFTPASSLRSFHDFYLVMPFMQTDLQKIMGMEFSEDKVQYLVYQMLKGLKY
IHSAGIVHRDLKPGNLAVNEDCELKILDFGLARHTDTEMTGYVVTRWYRAPEVILSWMHYNQTVDIWSVG
CIMAEMLTGKTLFKGKDYLDQLTQILKVTGVPGAEFVQKLKDKAAKSYIQSLPQSPKKDFTQLFPRASPQ
AADLLDKMLELDVDKRLTAAQALAHPFFEPFRDPEEETEAQQPFDDALEHEKLSVDEWKQHIYKEISNFS
PIARKDSRRRSGMKLQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC001992
ORF Size 1100 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC001992, AAH01992
RefSeq Size 1376 bp
RefSeq ORF 1100 bp
Locus ID 26415
Gene Summary Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK13 is one of the less studied p38 MAPK isoforms. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K. Involved in cytoskeletal remodeling through phosphorylation of MAPT and STMN1. Mediates UV irradiation induced up-regulation of the gene expression of CXCL14. Plays an important role in the regulation of epidermal keratinocyte differentiation, apoptosis and skin tumor development. Phosphorylates the transcriptional activator MYB in response to stress which leads to rapid MYB degradation via a proteasome-dependent pathway. MAPK13 also phosphorylates and down-regulates PRKD1 during regulation of insulin secretion in pancreatic beta cells.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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