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Kcnj1 (NM_019659) Mouse Tagged ORF Clone
CAT＃: MG205751
- TrueORF®
Kcnj1 (tGFP-tagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1)
ORF Plasmid: DDK
"NM_019659" in other vectors (4)
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CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Kir1.1; ROMK; Romk2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205751 representing NM_019659
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCAAACATCTTCGAAGATGGTTTGTCACTCACATATTTGGGCGTTCTCGGCAACGAGCAAGGTTGG TCTCCAAAGATGGAAGGTGTAACATCGAGTTTGGCAATGTAGATGCACAGTCGAGGTTTATATTCTTTGT GGATATCTGGACAACTGTACTTGACCTGAAATGGAGGTACAAAATGACCGTGTTCATCACAGCCTTCTTG GGGAGTTGGTTTCTCTTTGGTCTCCTGTGGTATGTCGTAGCCTATGTTCATAAGGATCTCCCAGAGTTCT ACCCACCTGACAACCGTACTCCTTGTGTGGAGAACATTAATGGCATGACATCAGCCTTTCTGTTTTCTCT AGAGACCCAAGTGACCATAGGTTACGGATTCAGGTTTGTGACAGAACAGTGTGCCACTGCCATTTTTCTG CTTATCTTCCAGTCTATTCTTGGAGTGATCATCAATTCTTTCATGTGTGGTGCCATATTAGCCAAGATCT CTAGACCCAAAAAACGTGCAAAGACCATTACATTCAGCAAGAATGCGGTGATCAGCAAACGTGGGGGGAA GCTCTGTCTCCTCATCCGAGTAGCAAATCTTAGGAAAAGCCTTCTGATTGGCAGTCACATATATGGTAAG CTTCTGAAGACTACCATCACACCTGAAGGAGAGACCATTATTTTGGATCAGACCAATATAAACTTTGTAG TTGATGCTGGCAATGAAAATTTGTTCTTCATTTCCCCACTGACAATCTACCACATTATTGACCACAACAG CCCTTTCTTCCACATGGCGGCAGAAACTCTTTCCCAACAGGACTTCGAGTTGGTTGTCTTTTTAGATGGC ACAGTAGAATCCACCAGTGCAACCTGCCAAGTCCGCACATCATACATCCCAGAAGAGGTGCTTTGGGGTT ACCGTTTTGTTCCCATCGTATCCAAGACCAAGGAAGGGAAATACCGAGTGGATTTCCATAACTTTGGTAA GACGGTGGAAGTGGAGACCCCTCATTGTGCCATGTGCCTCTATAATGAGAAAGATGCCAGGGCCAGGATG AAGAGAGGCTATGACAACCCTAACTTTGTCTTGTCAGAAGTTGATGAAACAGACGACACCCAAATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205751 representing NM_019659
Red=Cloning site Green=Tags(s) MFKHLRRWFVTHIFGRSRQRARLVSKDGRCNIEFGNVDAQSRFIFFVDIWTTVLDLKWRYKMTVFITAFL GSWFLFGLLWYVVAYVHKDLPEFYPPDNRTPCVENINGMTSAFLFSLETQVTIGYGFRFVTEQCATAIFL LIFQSILGVIINSFMCGAILAKISRPKKRAKTITFSKNAVISKRGGKLCLLIRVANLRKSLLIGSHIYGK LLKTTITPEGETIILDQTNINFVVDAGNENLFFISPLTIYHIIDHNSPFFHMAAETLSQQDFELVVFLDG TVESTSATCQVRTSYIPEEVLWGYRFVPIVSKTKEGKYRVDFHNFGKTVEVETPHCAMCLYNEKDARARM KRGYDNPNFVLSEVDETDDTQM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019659 |
| ORF Size | 1116 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019659.3 |
| RefSeq Size | 3102 bp |
| RefSeq ORF | 1119 bp |
| Locus ID | 56379 |
| UniProt ID | O88335 |
| Gene Summary | In the kidney, probably plays a major role in potassium homeostasis. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This channel is activated by internal ATP and can be blocked by external barium (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201126 | Kcnj1 (untagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1), transcript variant 2, (10ug) |
CNY 3,656.00 |
|
| MR205751 | Kcnj1 (Myc-DDK-tagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1), transcript variant 2 |
CNY 3,656.00 |
|
| MR205751L3 | Lenti ORF clone of Kcnj1 (Myc-DDK-tagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1), transcript variant 2 |
CNY 4,750.00 |
|
| MR205751L4 | Lenti ORF clone of Kcnj1 (mGFP-tagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 1 (Kcnj1), transcript variant 2 |
CNY 4,750.00 |
