Mapkapk2 (BC063064) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AA960234; MAPKAP-K2; MK-2; MK2; Rps6kc1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206027 representing BC063064 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTCGGGCTCTCCGGGCCAGACTCCGCCGGCGCCTTTCCCCAGCCCTCCACCGCCAGCCCCGGCCC AGCCGCCGCCGCCGTTCCCCCAGTTCCACGTCAAGTCGGGCCTGCAGATCCGAAAGAACGCCATCACCGA CGACTACAAGGTCACCAGCCAAGTGCTTGGCCTGGGCATCAACGGGAAGGTGCTGCGGATCTTCGACAAG AGAACCCAGCAAAAATTCGCCCTAAAGATGCTCCAGGACTGTCCGAAGGCGCGCAGAGAGGTGGAGCTGC ACTGGAGGGCCTCCCAGTGCCCACACATTGTGCACATCGTGGATGTCTATGAGAACCTGTATGCCGGGAG GAAGTGCCTGCTGATTGTCATGGAGTGTCTCGATGGTGGAGAGCTCTTTAGTCGAATCCAGGACCGAGGA GACCAGGCATTCACAGAAAGAGAGGCGTCAGAGATCATGAAGAGCATCGGCGAGGCCATCCAGTACCTGC ACTCGATCAACATTGCTCACCGGGATGTCAAGCCTGAGAACCTCTTATATACTTCCAAAAGGCCCAATGC CATTTTGAAACTCACTGATTTTGGCTTTGCCAAGGAAACCACCAGTCACAACTCTTTGACCACTCCGTGT TATACACCATACTATGTGGCTCCGGAAGTCCTGGGCCCGGAGAAGTATGACAAGTCCTGTGACATGTGGT CCTTGGGTGTCATCATGTATATTTTGCTGTGTGGGTATCCCCCCTTCTATTCCAATCACGGCCTTGCCAT CTCTCCGGGCATGAAGACTCGTATTCGAATGGGCCAGTATGAATTTCCTAACCCGGAGTGGTCAGAAGTA TCAGAAGAAGTGAAGATGCTTATCCGGAATCTGCTAAAAACAGAGCCCACCCAGAGAATGACCATCACAG AATTCATGAACCACCCCTGGATCATGCAATCTACGAAGGTCCCTCAGACTCCACTGCACACCAGCCGTGT CCTGAAGGAGGACAAGGAACGATGGGAGGATGTCAAGGAGGAGATGACCAGTGCCTTGGCCACGATGCGT GTTGACTATGAGCAGATCAAGATAAAGAAGATAGAAGACGCATCCAACCCTCTGCTTCTCAAGAGGCGGA AGAAAGCTCGTGCTGTGGAGGATGCGGCTCTCGCCCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206027 representing BC063064 Red=Cloning site Green=Tags(s) MLSGSPGQTPPAPFPSPPPPAPAQPPPPFPQFHVKSGLQIRKNAITDDYKVTSQVLGLGINGKVLRIFDK RTQQKFALKMLQDCPKARREVELHWRASQCPHIVHIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRG DQAFTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTSHNSLTTPC YTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSNHGLAISPGMKTRIRMGQYEFPNPEWSEV SEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKERWEDVKEEMTSALATMR VDYEQIKIKKIEDASNPLLLKRRKKARAVEDAALAH TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC063064
ORF Size	1160 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC063064, AAH63064
RefSeq Size	2586 bp
RefSeq ORF	1160 bp
Locus ID	17164
Gene Summary	Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Phosphorylates HSF1; leading to the interaction with HSP90 proteins and inhibiting HSF1 homotrimerization, DNA-binding and transactivation activities (By similarity). Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociation of HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impairment of their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to regulation of the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcripts. Phosphorylates CEP131 in response to cellular stress following ultraviolet irradiation which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites (By similarity). Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.[UniProtKB/Swiss-Prot Function]

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