Evl (BC059810) Mouse Tagged ORF Clone

CAT#: MG206142

  • TrueORF®

Evl (tGFP-tagged) - Mouse Ena-vasodilator stimulated phosphoprotein (cDNA clone MGC:67087 IMAGE:6828523)



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Mouse Protein / Lysate Request

CNY 3,710.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-Evl Antibody
    • 50 ug

CNY 5,200.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AI528774
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG206142 representing BC059810
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGAACAGAGTATCTGCCAAGCGCGGGCCTCCGTGATGGTCTACGATGACACCAGTAAGAAGTGGG
TACCGATCAAGCCTGGCCAGCAGGGATTCGGCCGGATCAACATCTACCACAACACTGCCAGCAGCACCTT
CAGAGTGGTCGGGGTCAAGCTACAGGACCAGCAGGTTGTGATCAATTATTCAATTGTTAAAGGGCTGAAG
TACAATCAGGCAACACCCACCTTCCATCAGTGGCGAGATGCCCGTCAGGTCTATGGCTTAAACTTTGCAA
GTAAGGAAGAAGCAACCACATTCTCCAATGCCATGCTCTTTGCCCTGAACATCATGAATTCCCAAGAAGG
AGGCCCCTCCACACAGCGTCAGGTGCAGAATGGCCCCTCTCCTGAGGAGATGGACATCCAGAGAAGACAA
GTAATGGAGCAGCAGCACCGCCAGGAGTCTCTGGAGAGGAGAATCTCGGCCACAGGGCCCATTCTCCCCC
CTGGGCATCCCTCATCGGCAGCCAGCACCACTCTCTCCTGTAGTGGACCTCCACCCCCGCCTCCACCCCC
AGTTCCACCTCCACCCACAGGGTCTACTCCCCCACCCCCACCCCCACTGCCAGCTGGAGGAGCCCAGGGG
ACCAACCATGATGAGAGCTCTGCATCAGGACTGGCTGCTGCTCTGGCGGGAGCCAAGCTAAGGAGGGTGC
AGCGGCCAGAAGATGCATCTGGAGGCTCCAGTCCTAGTGGGACTTCAAAGTCCGATGCCAACCGGGCAAG
CAGTGGGGGAGGTGGAGGAGGCCTCATGGAAGAAATGAACAAGCTGCTGGCTAAGAGGAGAAAGGCAGCC
TCCCAGACAGACAAGCCCGCTGACAGAAAGGAAGATGAGAGCCAAACGGAAGACCCTAGCACCTCCCCAT
CCCCAGGTACCCGAGCCACCAGCCAGCCACCTAATTCCTCAGAGGCTGGCAGAAAACCCTGGGAACGGAG
CAACTCGGTGGAGAAACCTGTGTCCTCGTTGCTGTCCAGGGTGAAGCCTGCTGGGAGTGTGAATGACGTG
GGCCTGGATGCCTTAGATTTGGACCGGATGAAACAGGAGATCCTGGAGGAGGTGGTTCGGGAGCTGCACA
AGGTGAAGGAGGAGATCATTGATGCCATCAGGCAGGAGCTAAGTGGGATCAGCACCACG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG206142 representing BC059810
Red=Cloning site Green=Tags(s)

MSEQSICQARASVMVYDDTSKKWVPIKPGQQGFGRINIYHNTASSTFRVVGVKLQDQQVVINYSIVKGLK
YNQATPTFHQWRDARQVYGLNFASKEEATTFSNAMLFALNIMNSQEGGPSTQRQVQNGPSPEEMDIQRRQ
VMEQQHRQESLERRISATGPILPPGHPSSAASTTLSCSGPPPPPPPPVPPPPTGSTPPPPPPLPAGGAQG
TNHDESSASGLAAALAGAKLRRVQRPEDASGGSSPSGTSKSDANRASSGGGGGGLMEEMNKLLAKRRKAA
SQTDKPADRKEDESQTEDPSTSPSPGTRATSQPPNSSEAGRKPWERSNSVEKPVSSLLSRVKPAGSVNDV
GLDALDLDRMKQEILEEVVRELHKVKEEIIDAIRQELSGISTT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC059810
ORF Size 1179 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC059810, AAH59810
RefSeq Size 2139 bp
RefSeq ORF 1181 bp
Locus ID 14026
Gene Summary Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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