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Gtf2h2 (BC016231) Mouse Tagged ORF Clone
CAT＃: MG206255
- TrueORF®
Gtf2h2 (tGFP-tagged) - Mouse general transcription factor II H, polypeptide 2 (cDNA clone MGC:28787 IMAGE:4488798)
ORF Plasmid: DDK
"BC016231" in other vectors (3)
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CNY 2,470.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Btf2p44, 44kDa |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206255 representing BC016231
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGAAGAACCTGAGAGAACCAAGCGGTGGGAAGGAGGCTATGAGAGAACCTGGGAAATTCTTAAAG AAGATGAAACTGGATCACTTAAAGCTACAATAGAAGATATTCTCTTCAAGGCAAAGAGGAAAAGAGTGTT TGAGCACCATGGACAAGTCCGACTTGGAATGATGCGCCACCTGTATGTGGTGGTGGATGGATCGAGAACA ATGGAAGATCAGGATTTAAAGCCCAATAGACTGACTTGCACTTTAAAGTTGCTGGAATACTTTGTAGAAG AATATTTTGATCAAAACCCTATCAGTCAGATTGGAATAATTGTAACTAAGAGTAAAAGAGCTGAAAAACT GACTGAACTCTCAGGAAACCCAAGGAAACATATAACATCTTTGAAGAAAGCTGTAGATATGACCTGCCAT GGAGAACCATCGCTCTATAATTCCTTAAGCATGGCTATGCAGACCCTAAAACACATGCCTGGACATACAA GTAGAGAAGTGCTCATCATCTTCAGCAGCCTCACCACCTGTGATCCATCTAATATTTACGATCTCATCAA GACCCTGAAGACAGCTAAAATTAGAGTGTCTGTTATTGGATTATCTGCGGAGGTTCGAGTTTGTACTGTA CTTGCTCGTGAAACTGGTGGCACATACCATGTTATCTTAGATGAAACCCATTACAAGGAGTTGTTGGCAC ATCATGTGAGCCCCCCTCCTGCCAGCTCAAGCTCCGAGTGCTCACTCATTCGCATGGGATTCCCTCAGCA TACCATTGCTTCTTTGTCTGATCAGGATGCAAAACCATCCTTCAGCATGGCGCATTTGGATAACAACAGC ACTGAGCCAGGGCTTACACTGGGAGGCTACTTCTGCCCACAGTGCCGAGCAAAGTACTGCGAGCTTCCTG TTGAATGTAAAATATGTGGTCTTACTTTGGTGTCTGCACCTCATTTGGCAAGATCTTACCATCATTTATT TCCTTTGGATGCTTTTCAAGAAATTTCCCTAGAAGAATATAAAGGAGAAAGGTTTTGTTATGGATGTCAG GGGGAATTGAAAGACCAACATGTCTATGTTTGCACAGTGTGCCAAAATGTTTTTTGTGTGGACTGTGATG TCTTTGTTCATGACTCTCTCCATTGTTGTCCTGGCTGTATTCATAAGATCCCAACTCCTTCAGGTCCATC TCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206255 representing BC016231
Red=Cloning site Green=Tags(s) MDEEPERTKRWEGGYERTWEILKEDETGSLKATIEDILFKAKRKRVFEHHGQVRLGMMRHLYVVVDGSRT MEDQDLKPNRLTCTLKLLEYFVEEYFDQNPISQIGIIVTKSKRAEKLTELSGNPRKHITSLKKAVDMTCH GEPSLYNSLSMAMQTLKHMPGHTSREVLIIFSSLTTCDPSNIYDLIKTLKTAKIRVSVIGLSAEVRVCTV LARETGGTYHVILDETHYKELLAHHVSPPPASSSSECSLIRMGFPQHTIASLSDQDAKPSFSMAHLDNNS TEPGLTLGGYFCPQCRAKYCELPVECKICGLTLVSAPHLARSYHHLFPLDAFQEISLEEYKGERFCYGCQ GELKDQHVYVCTVCQNVFCVDCDVFVHDSLHCCPGCIHKIPTPSGPSL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC016231 |
| ORF Size | 1194 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC016231, AAH16231 |
| RefSeq Size | 1643 bp |
| RefSeq ORF | 1196 bp |
| Locus ID | 23894 |
| Gene Summary | Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. The N-terminus of GTF2H2 interacts with and regulates XPD whereas an intact C-terminus is required for a successful escape of RNAP II form the promoter.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR206255 | Gtf2h2 (Myc-DDK-tagged) - Mouse general transcription factor II H, polypeptide 2 (cDNA clone MGC:28787 IMAGE:4488798) |
CNY 3,656.00 |
|
| MR206255L3 | Lenti ORF clone of Gtf2h2 (Myc-DDK-tagged) - Mouse general transcription factor II H, polypeptide 2 (cDNA clone MGC:28787 IMAGE:4488798) |
CNY 4,180.00 |
|
| MR206255L4 | Lenti ORF clone of Gtf2h2 (mGFP-tagged) - Mouse general transcription factor II H, polypeptide 2 (cDNA clone MGC:28787 IMAGE:4488798) |
CNY 4,180.00 |
