Gtf2h2 (BC016231) Mouse Tagged ORF Clone

CAT#: MG206255

  • TrueORF®

Gtf2h2 (tGFP-tagged) - Mouse general transcription factor II H, polypeptide 2 (cDNA clone MGC:28787 IMAGE:4488798)

ORF Plasmid: DDK tGFP



  "BC016231" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 2,470.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Btf2p44, 44kDa
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG206255 representing BC016231
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGAAGAACCTGAGAGAACCAAGCGGTGGGAAGGAGGCTATGAGAGAACCTGGGAAATTCTTAAAG
AAGATGAAACTGGATCACTTAAAGCTACAATAGAAGATATTCTCTTCAAGGCAAAGAGGAAAAGAGTGTT
TGAGCACCATGGACAAGTCCGACTTGGAATGATGCGCCACCTGTATGTGGTGGTGGATGGATCGAGAACA
ATGGAAGATCAGGATTTAAAGCCCAATAGACTGACTTGCACTTTAAAGTTGCTGGAATACTTTGTAGAAG
AATATTTTGATCAAAACCCTATCAGTCAGATTGGAATAATTGTAACTAAGAGTAAAAGAGCTGAAAAACT
GACTGAACTCTCAGGAAACCCAAGGAAACATATAACATCTTTGAAGAAAGCTGTAGATATGACCTGCCAT
GGAGAACCATCGCTCTATAATTCCTTAAGCATGGCTATGCAGACCCTAAAACACATGCCTGGACATACAA
GTAGAGAAGTGCTCATCATCTTCAGCAGCCTCACCACCTGTGATCCATCTAATATTTACGATCTCATCAA
GACCCTGAAGACAGCTAAAATTAGAGTGTCTGTTATTGGATTATCTGCGGAGGTTCGAGTTTGTACTGTA
CTTGCTCGTGAAACTGGTGGCACATACCATGTTATCTTAGATGAAACCCATTACAAGGAGTTGTTGGCAC
ATCATGTGAGCCCCCCTCCTGCCAGCTCAAGCTCCGAGTGCTCACTCATTCGCATGGGATTCCCTCAGCA
TACCATTGCTTCTTTGTCTGATCAGGATGCAAAACCATCCTTCAGCATGGCGCATTTGGATAACAACAGC
ACTGAGCCAGGGCTTACACTGGGAGGCTACTTCTGCCCACAGTGCCGAGCAAAGTACTGCGAGCTTCCTG
TTGAATGTAAAATATGTGGTCTTACTTTGGTGTCTGCACCTCATTTGGCAAGATCTTACCATCATTTATT
TCCTTTGGATGCTTTTCAAGAAATTTCCCTAGAAGAATATAAAGGAGAAAGGTTTTGTTATGGATGTCAG
GGGGAATTGAAAGACCAACATGTCTATGTTTGCACAGTGTGCCAAAATGTTTTTTGTGTGGACTGTGATG
TCTTTGTTCATGACTCTCTCCATTGTTGTCCTGGCTGTATTCATAAGATCCCAACTCCTTCAGGTCCATC
TCTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG206255 representing BC016231
Red=Cloning site Green=Tags(s)

MDEEPERTKRWEGGYERTWEILKEDETGSLKATIEDILFKAKRKRVFEHHGQVRLGMMRHLYVVVDGSRT
MEDQDLKPNRLTCTLKLLEYFVEEYFDQNPISQIGIIVTKSKRAEKLTELSGNPRKHITSLKKAVDMTCH
GEPSLYNSLSMAMQTLKHMPGHTSREVLIIFSSLTTCDPSNIYDLIKTLKTAKIRVSVIGLSAEVRVCTV
LARETGGTYHVILDETHYKELLAHHVSPPPASSSSECSLIRMGFPQHTIASLSDQDAKPSFSMAHLDNNS
TEPGLTLGGYFCPQCRAKYCELPVECKICGLTLVSAPHLARSYHHLFPLDAFQEISLEEYKGERFCYGCQ
GELKDQHVYVCTVCQNVFCVDCDVFVHDSLHCCPGCIHKIPTPSGPSL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC016231
ORF Size 1196 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC016231, AAH16231
RefSeq Size 1643 bp
RefSeq ORF 1196 bp
Locus ID 23894
Gene Summary Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. The N-terminus of GTF2H2 interacts with and regulates XPD whereas an intact C-terminus is required for a successful escape of RNAP II form the promoter.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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