P4ha3 (BC082538) Mouse Tagged ORF Clone
CAT#: MG206351
- TrueORF®
P4ha3 (tGFP-tagged) - Mouse procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III (cDNA clone
ORF Plasmid: DDK
"BC082538" in other vectors (4)
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CNY 2,470.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | D930031A02Rik |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG206351 representing BC082538
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCTGCAGGATGTGTACATGCTCAACGTGAAGGGCTTGGCTCGAGGCGTCTTCCAGAGGGTCACGG GCTCCTCCATCACCGACCTGTACAGTCCCAGGCAGCTCTTCTCTCTCACAGCGGATGACTGCTTCCAAGT TGGCAAGGTGGCCTATGACACAGGGGATTACTACCATGCCATCCCATGGCTGGAGGAAGCTGTCAGTCTC TTCCGGAGAGCCCACGGCGAGTGGAAGACAGAGGACGAGGCCAGTCTGGAAGACGCCCTGGATTACTTGG CCTTTGCCTGTTTCCAGGTAGGAAATGTTTCATGTGCCCTCAGCCTCTCTCGAGAGTTCCTTGTCTACAG CCCAGACAATAAGAGGATGGCCAGGAATGTGTTGAAATATGAAAGGCTCTTGGCTGAGAATGGTCACCAG ATGGCGGCTGAGACTGCAATTCAGAGGCCCAACGTACCCCACCTGCAGACCAGGGACACCTACGAGGGGC TATGCCAGACCCTGGGCTCCCAGCCAACACACTACCAAATCCCCAGCCTCTACTGCTCCTATGAGACCAA TTCCAGCCCCTACCTACTGCTCCAGCCTGCCCGAAAGGAGGTTGTCCACCTACGGCCGCTCATAGCTCTC TACCATGACTTTGTCAGTGACGAGGAAGCTCAGAAAATCCGAGAGCTTGCAGAACCGTGGCTCCAGAGAT CAGTGGTGGCTTCGGGGGAAAAGCAGTTGCAAGTGGAGTACCGCATCAGCAAAAGTGCCTGGCTAAAGGA CACGGTTGATCCAATGCTGGTGACCCTTGACCACCGCATTGCGGCCCTCACAGGCCTTGACATCCAGCCT CCCTACGCAGAGTATCTCCAGGTGGTGAACTATGGAATCGGAGGGCACTATGAGCCTCACTTTGACCATG CTACGTCGCCCAGCAGCCCCCTGTACAGAATGAAGTCTGGGAACCGAGTGGCAACGTTTATGATCTATCT GAGCTCCGTAGAAGCTGGAGGAGCCACGGCTTTTATCTATGGCAACTTCAGTGTGCCTGTGGTTAAGAAT GCTGCACTGTTTTGGTGGAACCTGCACAGGAGCGGAGAGGGGGATGGTGACACACTTCATGCTGGCTGTC CTGTGCTGGTGGGAGACAAGTGGGTGGCCAACAAGTGGATCCACGAGTATGGACAGGAATTCCGAAGACC CTGCAGCACCAACCCTGAAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206351 representing BC082538
Red=Cloning site Green=Tags(s) MRLQDVYMLNVKGLARGVFQRVTGSSITDLYSPRQLFSLTADDCFQVGKVAYDTGDYYHAIPWLEEAVSL FRRAHGEWKTEDEASLEDALDYLAFACFQVGNVSCALSLSREFLVYSPDNKRMARNVLKYERLLAENGHQ MAAETAIQRPNVPHLQTRDTYEGLCQTLGSQPTHYQIPSLYCSYETNSSPYLLLQPARKEVVHLRPLIAL YHDFVSDEEAQKIRELAEPWLQRSVVASGEKQLQVEYRISKSAWLKDTVDPMLVTLDHRIAALTGLDIQP PYAEYLQVVNYGIGGHYEPHFDHATSPSSPLYRMKSGNRVATFMIYLSSVEAGGATAFIYGNFSVPVVKN AALFWWNLHRSGEGDGDTLHAGCPVLVGDKWVANKWIHEYGQEFRRPCSTNPED TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC082538 |
ORF Size | 1214 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC082538, AAH82538 |
RefSeq Size | 2426 bp |
RefSeq ORF | 1214 bp |
Locus ID | 320452 |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC218685 | P4ha3 (untagged) - Mouse procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III (cDNA clone, (10ug) |
CNY 6,940.00 |
|
MR206351 | P4ha3 (Myc-DDK-tagged) - Mouse procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III (cDNA clone |
CNY 3,656.00 |
|
MR206351L3 | Lenti ORF clone of P4ha3 (Myc-DDK-tagged) - Mouse procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III (cDNA clone |
CNY 4,180.00 |
|
MR206351L4 | Lenti ORF clone of P4ha3 (mGFP-tagged) - Mouse procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III (cDNA clone |
CNY 4,180.00 |