Got1 (BC002057) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AI789014; cAspAT; cCAT; Got-1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206497 representing BC002057 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCTCCATCAGTCTTTGCCCAGGTTCCGCAAGCTCCTCCGGTTCTGGTCTTTAAGCTCACTGCGG ACTTCCGGGATGATCCAGATCCCCGCAAGGTTAACCTCGGCGTGGGAGCGTACCGCACAGATGAATCTCA GCCCTGGGTTTTGCCAGTAGTGAGGAAGGTCGAACAGAAGATTGCTAATGACAACAGCCTCAACCACGAG TACCTGCCCATCCTGGGCCTGGCAGAGTTCCGGAGCTGTGCTTCTCGCCTAGTTCTTGGGGACAACAGCC CGGCTATCAGGGAGAATCGGGTTGGAGGGGTGCAGTCTTTGGGAGGGACAGGCGCTCTTCGGATTGGAGC TGACTTCTTAGGGCGATGGTACAATGGTACAGATAACAAGAACACACCAATCTACGTATCATCGCCAACC TGGGAGAACCATAATGCTGTGTTTTCTGCCGCCGGTTTTAAGGACATTCGGCCCTATTGCTACTGGGATG CGGAGAAGAGAGGACTGGACCTCCAGGGTTTCCTGAATGATCTGGAGAATGCCCCCGAGTTCTCCATCTT TGTCCTCCATGCCTGTGCGCACAACCCAACAGGGACCGACCCGACTCCAGAGCAGTGGAAGCAGATCGCT GCTGTCATGCAGCGCCGTTTTCTGTTCCCCTTCTTTGACTCAGCCTATCAGGGCTTTGCATCTGGAGACC TAGAGAAAGATGCGTGGGCTATTCGCTATTTTGTGTCTGAAGGCTTCGAGCTCTTCTGTGCCCAGTCCTT CTCCAAGAACTTCGGGCTCTACAATGAGAGAGTGGGGAATCTGACCGTGGTCGGAAAAGAGTCTGACAGC GTCCTGCGGGTCCTTTCCCAGATGGAGAAGATTGTACGAATCACCTGGTCCAATCCCCCAGCCCAAGGAG CTCGGATTGTGGCCGCCACCCTCTCTGACCCGGAGCTCTTTAAGGAGTGGAAAGGTAACGTGAAGACAAT GGCTGACCGGATTCTGACCATGAGATCCGAACTCAGGGCAAGACTAGAAGCTCTCAAGACCCCCGGGACT TGGTCTCACATCACTGAGCAGATTGGAATGTTCAGTTTCACCGGCTTGAACCCCAAGCAGGTCGAGTATT TGGTCAACGAGAAGCATATCTATCTCCTGCCGAGTGGTCGGATCAACATGTGCGGCTTGACCACCAAGAA CCTAGATTACGTCGCTACCTCCATCCATGAAGCCGTCACCAAAATCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206497 representing BC002057 Red=Cloning site Green=Tags(s) MAPPSVFAQVPQAPPVLVFKLTADFRDDPDPRKVNLGVGAYRTDESQPWVLPVVRKVEQKIANDNSLNHE YLPILGLAEFRSCASRLVLGDNSPAIRENRVGGVQSLGGTGALRIGADFLGRWYNGTDNKNTPIYVSSPT WENHNAVFSAAGFKDIRPYCYWDAEKRGLDLQGFLNDLENAPEFSIFVLHACAHNPTGTDPTPEQWKQIA AVMQRRFLFPFFDSAYQGFASGDLEKDAWAIRYFVSEGFELFCAQSFSKNFGLYNERVGNLTVVGKESDS VLRVLSQMEKIVRITWSNPPAQGARIVAATLSDPELFKEWKGNVKTMADRILTMRSELRARLEALKTPGT WSHITEQIGMFSFTGLNPKQVEYLVNEKHIYLLPSGRINMCGLTTKNLDYVATSIHEAVTKIQ TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC002057
ORF Size	1241 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC002057, AAH02057
RefSeq Size	1985 bp
RefSeq ORF	1241 bp
Locus ID	14718
Gene Summary	Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain (By similarity).[UniProtKB/Swiss-Prot Function]

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