Casq2 (BC092229) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AA033488; AW146219; cCSQ; Csq2; ESTM52
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206556 representing BC092229 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAGGATTTACCTGCTCATGGTGGGGGTTTATCTGCTGTCCCTGAGCGGGGCAGAAGAGGGGCTGA ACTTCCCCACGTACGATGGGAAAGACCGAGTGGTCAGCCTTTCTGAGAAGAACCTCAAGCAGATGTTGAA GAGATATGATTTGCTCTGTCTCTATTACCACGAACCTGTGTCTTCAGACAAGGTCTCACAAAAACAGTTC CAGCTGAAGGAGATTGTACTGGAGCTTGTGGCCCAGGTCCTGGAACATAAAAACATAGGCTTTGTGATGG TGGATTCGAGGAAAGAGGCCAAGCTTGCTAAGAGGCTGGGATTCAGTGAAGAAGGAAGCCTGTATGTTCT GAAGGGTGACCGCACGATTGAGTTTGACGGGGAGTTCGCAGCAGATGTCTTAGTGGAATTTCTCTTGGAT CTCATTGAAGACCCAGTGGAGATCGTGAATAACAAGCTGGAGGTCCAGGCCTTTGAGCGCATCGAGGACC AGACCAAGCTCCTTGGCTTCTTCAAGAATGAGGACTCAGAATATTACAAAGCATTCCAAGAGGCAGCTGA ACACTTCCAGCCTTACATCAAGTTCTTTGCCACCTTTGACAAGGCGGTGGCAAAGAAGTTATCCTTGAAG ATGAACGAAGTTGGCTTCTATGAGCCATTTATGGATGAGCCCAACGTCATCCCTAACAAACCGTACACAG AAGAGGAGCTTGTGGAGTTTGTGAAGGAACATCAAAGACCCACCCTACGTCGCTTGCGCCCAGAGGACAT GTTTGAAACATGGGAAGACGACTTGAATGGGATCCACATCGTGGCCTTTGCGGAGAAGAGTGACCCAGAT GGCTATGAGTTCCTAGAGATCCTGAAACAGGTTGCCCGGGACAACACTGACAATCCTGACTTGAGCATCT TGTGGATTGACCCAGATGACTTTCCACTGCTTGTTGCTTACTGGGAGAAGACTTTCAAGATTGACCTGTT CAAGCCACAGATTGGGGTGGTGAATGTAACCGATGCTGACAGTATCTGGATGGAGATCCCAGATGATGAC GACCTGCCCACAGCTGAGGAGCTGGAAGACTGGATTGAAGATGTGCTTTCTGGAAAGATCAACACTGAAG ATGATGACAATGAAGATGAAGATGATGATGGTGATGACAACGATGACGATGATGATGACGACGATGATAA TGATAATTCTGATGAGGATAATGAAGACAGTGATGATGACGATGATGACGATGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206556 representing BC092229 Red=Cloning site Green=Tags(s) MKRIYLLMVGVYLLSLSGAEEGLNFPTYDGKDRVVSLSEKNLKQMLKRYDLLCLYYHEPVSSDKVSQKQF QLKEIVLELVAQVLEHKNIGFVMVDSRKEAKLAKRLGFSEEGSLYVLKGDRTIEFDGEFAADVLVEFLLD LIEDPVEIVNNKLEVQAFERIEDQTKLLGFFKNEDSEYYKAFQEAAEHFQPYIKFFATFDKAVAKKLSLK MNEVGFYEPFMDEPNVIPNKPYTEEELVEFVKEHQRPTLRRLRPEDMFETWEDDLNGIHIVAFAEKSDPD GYEFLEILKQVARDNTDNPDLSILWIDPDDFPLLVAYWEKTFKIDLFKPQIGVVNVTDADSIWMEIPDDD DLPTAEELEDWIEDVLSGKINTEDDDNEDEDDDGDDNDDDDDDDDDNDNSDEDNEDSDDDDDDDE TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC092229
ORF Size	1247 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC092229, AAH92229
RefSeq Size	2457 bp
RefSeq ORF	1247 bp
Locus ID	12373
Gene Summary	Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. Calcium ions are bound by clusters of acidic residues at the protein surface, especially at the interface between subunits. Can bind around 60 Ca(2+) ions. Regulates the release of lumenal Ca(2+) via the calcium release channel RYR2; this plays an important role in triggering muscle contraction. Plays a role in excitation-contraction coupling in the heart and in regulating the rate of heart beats.[UniProtKB/Swiss-Prot Function]

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