Wnt10a (NM_009518) Mouse Tagged ORF Clone
CAT#: MG206595
- TrueORF®
Wnt10a (tGFP-tagged) - Mouse wingless related MMTV integration site 10a (Wnt10a)
ORF Plasmid: DDK
"NM_009518" in other vectors (4)
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CNY 7,088.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009518 |
ORF Size | 1251 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009518.2 |
RefSeq Size | 2487 bp |
RefSeq ORF | 1254 bp |
Locus ID | 22409 |
UniProt ID | P70701 |
Gene Summary | Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt/beta-catenin signaling pathway. Plays a role in normal ectoderm development. Required for normal tooth development. Required for normal postnatal development and maintenance of tongue papillae and sweat ducts. Required for normal proliferation of basal cells in tongue filiform papillae, plantar epithelium and sweat ducts. Required for normal expression of keratins in tongue papillae. Required for normal expression of KRT9 in foot plant epithelium. Required for normal hair follicle function.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Lhx6 regulates canonical Wnt signaling to control the fate of mesenchymal progenitor cells during mouse molar root patterning
,He, J;Jing, J;Feng, J;Han, X;Yuan, Y;Guo, T;Pei, F;Ma, Y;Cho, C;Ho, TV;Chai, Y;,
PLoS genetics
,PubMed ID 33596195
[WNT10A]
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Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201512 | Wnt10a (untagged) - Mouse wingless related MMTV integration site 10a (Wnt10a), (10ug) |
CNY 3,656.00 |
|
MR206595 | Wnt10a (Myc-DDK-tagged) - Mouse wingless related MMTV integration site 10a (Wnt10a) |
CNY 5,488.00 |
|
MR206595L3 | Lenti ORF clone of Wnt10a (Myc-DDK-tagged) - Mouse wingless related MMTV integration site 10a (Wnt10a) |
CNY 4,750.00 |
|
MR206595L4 | Lenti ORF clone of Wnt10a (mGFP-tagged) - Mouse wingless related MMTV integration site 10a (Wnt10a) |
CNY 7,888.00 |