Chst12 (NM_021528) Mouse Tagged ORF Clone
CAT#: MG206647
- TrueORF®
Chst12 (tGFP-tagged) - Mouse carbohydrate sulfotransferase 12 (Chst12)
ORF Plasmid: DDK
"NM_021528" in other vectors (3)
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CNY 3,140.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AI595374; C4S-2; C4ST2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG206647 representing NM_021528
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCAAGCCGCGGCTCTTCCGGCTGTGGCTGGTACTAGGGTCGGCTCTCATGATCCTTTTGATCATTG TATATTGGGACAACGTGGGAACCGCCCACTTCTATCTGCACACGTCTCTCTCCAGGCCACACATCCTAGA ACCCCTTCCCACCCAGGGATTGGTGGAGGAGAACGTGTTCACATCTGACGTGGATGAGTTTTTGGATACT CTCCTTAGTTCTGACGCGAAGCACAACGACCTTTCCAGGAGAAAAACTGAGCAGCCCCCGGCGCCCGCCC CCAGCAAGCCAGTCTTGAGCCACATGGAGGAGAACGTGAGAGGCTACGACTGGTCCACTCATGATGCCCA TCAGAACCCTGACCGGGACAGGCAGCAGGCCGAGAGGAGGAGCCTGCTGAGAGACTTCTGTGCCAACGCC AGCCTGGCATTCCCCACCAAGGACCGCTCTTTTGACGACATCCCCAACTACGAACTGAACCACCTGATCG TGGACGACCGCCACGGGGTCATCTACTGCTACGTGCCCAAGGTGGCCTGCACCAACTGGAAGCGAGTGAT GATCGTGCTGAGCGAGAGCCTGCTGGACCGGGGCAGCCCCTACCGAGACCCCCTGGACATCCCCCGGGAA CACGTGCACAACACCAGCACGCACCTCACCTTCAACAAGTTCTGGCGCCGCTACGGAAAGTTCTCCCGTC ACCTCATGAAGGTGAAGCTGAAGAAGTACACCAAGTTCCTGTTCGTGCGCGACCCCTTTGTGCGCCTCAT CTCAGCCTTCCGCAGCAAGTTCGAGCTGGAGAACGAAGAGTTTTACCGCAAGTTCGCGGTGCCCATGCTC CGACTGTACGCCAACCACACCAGCCTGCCCGCCTCGGTGAGTGAGGCTTTCAGCGCCGGGCTCAAGGTCT CCTTCGCCAACTTCATCCAGTACCTCCTAGACCCACACACGGAGAAGCTGGCGCCTTTCAACGAGCACTG GCGACAGGTGTACCGCCTCTGCCACCCGTGCCAGATAGACTATGACTTCGTGGGGAAGCTGGAGACGCTC GATGAGGACGCTGCCCAGCTCCTGAGGTTCCTCAAGGTAGACTCCCAGCTCCACTTCCCCCCCAGTTATC GGAACAGGACGGCCAGCAGCTGGGAGGAAGACTGGTTTGCCAACATCCCCCTGGCATGGAGGCAACAGCT CTATAAACTCTACGAGGCCGACTTTGTTCTCTTTGGCTACCCCAAGCCAGAAAACCTGCTCAGGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206647 representing NM_021528
Red=Cloning site Green=Tags(s) MTKPRLFRLWLVLGSALMILLIIVYWDNVGTAHFYLHTSLSRPHILEPLPTQGLVEENVFTSDVDEFLDT LLSSDAKHNDLSRRKTEQPPAPAPSKPVLSHMEENVRGYDWSTHDAHQNPDRDRQQAERRSLLRDFCANA SLAFPTKDRSFDDIPNYELNHLIVDDRHGVIYCYVPKVACTNWKRVMIVLSESLLDRGSPYRDPLDIPRE HVHNTSTHLTFNKFWRRYGKFSRHLMKVKLKKYTKFLFVRDPFVRLISAFRSKFELENEEFYRKFAVPML RLYANHTSLPASVSEAFSAGLKVSFANFIQYLLDPHTEKLAPFNEHWRQVYRLCHPCQIDYDFVGKLETL DEDAAQLLRFLKVDSQLHFPPSYRNRTASSWEEDWFANIPLAWRQQLYKLYEADFVLFGYPKPENLLRD TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_021528 |
ORF Size | 1257 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021528.2, NP_067503.2 |
RefSeq Size | 1785 bp |
RefSeq ORF | 1260 bp |
Locus ID | 59031 |
UniProt ID | Q99LL3 |
Gene Summary | Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Activity toward partially desulfated dermatan sulfate is however lower. Does not form 4, 6-di-O-sulfated GalNAc when chondroitin sulfate C is used as an acceptor (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR206647 | Chst12 (Myc-DDK-tagged) - Mouse carbohydrate sulfotransferase 12 (Chst12) |
CNY 2,850.00 |
|
MR206647L3 | Lenti ORF clone of Chst12 (Myc-DDK-tagged) - Mouse carbohydrate sulfotransferase 12 (Chst12) |
CNY 4,750.00 |
|
MR206647L4 | Lenti ORF clone of Chst12 (mGFP-tagged) - Mouse carbohydrate sulfotransferase 12 (Chst12) |
CNY 4,750.00 |