Crbn (BC086488) Mouse Tagged ORF Clone

CAT#: MG206875

  • TrueORF®

Crbn (tGFP-tagged) - Mouse cereblon (cDNA clone MGC:103113 IMAGE:30443656)

ORF Plasmid: DDK tGFP



  "BC086488" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,760.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 2610203G15Rik; 2900045O07Rik; AF229032; AW108261; piL
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG206875 representing BC086488
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAAACCACCTGCCGCTTCTGCCTGACAGTGAAGATGAAGATGATGAAATTGAAATGGAAGTTGAAG
ACCAAGATAGTAAAGAAGCCAGAAAACCGAATATCATAAACTTTGACACCAGTCTGCCAACCTCACATAC
ATACCTGGGAGCTGATATGGAGGAGTTCCACGGGAGAACTTTGCATGACGACGACAGCTGCCAGGTGATC
CCAGTCCTTCCTGAGGTGCTGATGATCCTGATTCCTGGGCAGACACTCCCACTGCAGCTCTCTCACCCAC
AGGAAGTCAGCATGGTGCGGAACTTAATCCAGAAAGACAGGACCTTTGCAGTCCTTGCATACAGTAATGT
GCAAGAAAGGGAAGCACAGTTTGGGACAACAGCAGAGATCTATGCCTATCGAGAAGAGCAGGAGTTTGGA
ATTGAAGTAGTGAAAGTGAAAGCAATTGGAAGGCAGCGGTTCAAGGTCCTCGAACTTCGAACACAGTCAG
ATGGAATCCAGCAAGCTAAAGTGCAGATTTTGCCAGAGTGTGTGTTGCCGTCAACCATGTCTGCAGTGCA
GGTAGAATCACTCAATAAGTGCCAGGTATTTCCTTCAAAACCCATCTCCTGGGAAGACCAGTATTCATGT
AAATGGTGGCAGAAATACCAGAAGAGAAAGTTTCACTGTGCAAATCTAACATCATGGCCTCGCTGGCTGT
ATTCATTATATGATGCTGAAACATTAATGGATAGAATTAAGAAACAGCTACGTGAATGGGATGAAAATCT
CAAAGATGATTCTCTTCCTGAAAATCCAATAGACTTTTCTTACAGAGTAGCTGCTTGTCTTCCTATTGAT
GATGTATTGAGAATTCAGCTCCTTAAAATCGGCAGTGCTATTCAACGGCTTCGCTGTGAATTGGACATCA
TGAACAAATGTACTTCCCTTTGCTGTAAACAATGTCAAGAAACAGAAATAACGACAAAGAATGAAATATT
TAGTTTATCCTTATGTGGTCCAATGGCAGCATATGTGAATCCTCATGGATATGTACATGAGACACTGACT
GTGTATAAAGCGTCCAACCTGAATCTGATAGGCCGGCCTTCTACAGTGCACAGCTGGTTTCCCGGGTATG
CATGGACCATTGCCCAGTGCAAGATCTGTGCAAGCCATATTGGATGGAAATTTACAGCCACAAAAAAAGA
CATGTCACCTCAAAAATTTTGGGGCTTAACTCGCTCTGCTCTGTTACCCACAATTCCAGAGACTGAAGAT
GAAATAAGTCCAGACAAAGTAATACTTTGTTTA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG206875 representing BC086488
Red=Cloning site Green=Tags(s)

MGNHLPLLPDSEDEDDEIEMEVEDQDSKEARKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVI
PVLPEVLMILIPGQTLPLQLSHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQEFG
IEVVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQVESLNKCQVFPSKPISWEDQYSC
KWWQKYQKRKFHCANLTSWPRWLYSLYDAETLMDRIKKQLREWDENLKDDSLPENPIDFSYRVAACLPID
DVLRIQLLKIGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYVNPHGYVHETLT
VYKASNLNLIGRPSTVHSWFPGYAWTIAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLPTIPETED
EISPDKVILCL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC086488
ORF Size 1295 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC086488, AAH86488
RefSeq Size 2107 bp
RefSeq ORF 1295 bp
Locus ID 58799
Gene Summary This gene encodes a protein with a Lon protease domain, a "regulators of G protein-signaling" (RGS)-like domain and a leucine zipper. It has been proposed to regulate the assembly and surface expression of large-conductance calcium-activated potassium channels in brain and to bind thalidomide. In humans mutation in this gene causes autosomal recessive nonsyndromic cognitive disability. In mouse deficiency of this gene serves as a model to study the molecular mechanisms governing learning and memory as they relate to intellectual disability. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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