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Crbn (BC086488) Mouse Tagged ORF Clone
CAT＃: MR206875
- TrueORF®
Crbn (Myc-DDK-tagged) - Mouse cereblon (cDNA clone MGC:103113 IMAGE:30443656)
ORF Plasmid: tGFP
"BC086488" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2610203G15Rik; 2900045O07Rik; AF229032; AW108261; piL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206875 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAACCACCTGCCGCTTCTGCCTGACAGTGAAGATGAAGATGATGAAATTGAAATGGAAGTTGAAG ACCAAGATAGTAAAGAAGCCAGAAAACCGAATATCATAAACTTTGACACCAGTCTGCCAACCTCACATAC ATACCTGGGAGCTGATATGGAGGAGTTCCACGGGAGAACTTTGCATGACGACGACAGCTGCCAGGTGATC CCAGTCCTTCCTGAGGTGCTGATGATCCTGATTCCTGGGCAGACACTCCCACTGCAGCTCTCTCACCCAC AGGAAGTCAGCATGGTGCGGAACTTAATCCAGAAAGACAGGACCTTTGCAGTCCTTGCATACAGTAATGT GCAAGAAAGGGAAGCACAGTTTGGGACAACAGCAGAGATCTATGCCTATCGAGAAGAGCAGGAGTTTGGA ATTGAAGTAGTGAAAGTGAAAGCAATTGGAAGGCAGCGGTTCAAGGTCCTCGAACTTCGAACACAGTCAG ATGGAATCCAGCAAGCTAAAGTGCAGATTTTGCCAGAGTGTGTGTTGCCGTCAACCATGTCTGCAGTGCA GGTAGAATCACTCAATAAGTGCCAGGTATTTCCTTCAAAACCCATCTCCTGGGAAGACCAGTATTCATGT AAATGGTGGCAGAAATACCAGAAGAGAAAGTTTCACTGTGCAAATCTAACATCATGGCCTCGCTGGCTGT ATTCATTATATGATGCTGAAACATTAATGGATAGAATTAAGAAACAGCTACGTGAATGGGATGAAAATCT CAAAGATGATTCTCTTCCTGAAAATCCAATAGACTTTTCTTACAGAGTAGCTGCTTGTCTTCCTATTGAT GATGTATTGAGAATTCAGCTCCTTAAAATCGGCAGTGCTATTCAACGGCTTCGCTGTGAATTGGACATCA TGAACAAATGTACTTCCCTTTGCTGTAAACAATGTCAAGAAACAGAAATAACGACAAAGAATGAAATATT TAGTTTATCCTTATGTGGTCCAATGGCAGCATATGTGAATCCTCATGGATATGTACATGAGACACTGACT GTGTATAAAGCGTCCAACCTGAATCTGATAGGCCGGCCTTCTACAGTGCACAGCTGGTTTCCCGGGTATG CATGGACCATTGCCCAGTGCAAGATCTGTGCAAGCCATATTGGATGGAAATTTACAGCCACAAAAAAAGA CATGTCACCTCAAAAATTTTGGGGCTTAACTCGCTCTGCTCTGTTACCCACAATTCCAGAGACTGAAGAT GAAATAAGTCCAGACAAAGTAATACTTTGTTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206875 protein sequence
Red=Cloning site Green=Tags(s) MGNHLPLLPDSEDEDDEIEMEVEDQDSKEARKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVI PVLPEVLMILIPGQTLPLQLSHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQEFG IEVVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQVESLNKCQVFPSKPISWEDQYSC KWWQKYQKRKFHCANLTSWPRWLYSLYDAETLMDRIKKQLREWDENLKDDSLPENPIDFSYRVAACLPID DVLRIQLLKIGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYVNPHGYVHETLT VYKASNLNLIGRPSTVHSWFPGYAWTIAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLPTIPETED EISPDKVILCL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC086488 |
| ORF Size | 1293 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC086488, AAH86488 |
| RefSeq Size | 2107 bp |
| RefSeq ORF | 1295 bp |
| Locus ID | 58799 |
| MW | 49.5 kDa |
| Gene Summary | This gene encodes a protein with a Lon protease domain, a "regulators of G protein-signaling" (RGS)-like domain and a leucine zipper. It has been proposed to regulate the assembly and surface expression of large-conductance calcium-activated potassium channels in brain and to bind thalidomide. In humans mutation in this gene causes autosomal recessive nonsyndromic cognitive disability. In mouse deficiency of this gene serves as a model to study the molecular mechanisms governing learning and memory as they relate to intellectual disability. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218344 | Crbn (untagged) - Mouse cereblon (cDNA clone MGC:103113 IMAGE:30443656), (10ug) |
CNY 6,940.00 |
|
| MG206875 | Crbn (tGFP-tagged) - Mouse cereblon (cDNA clone MGC:103113 IMAGE:30443656) |
CNY 2,760.00 |
|
| MR206875L3 | Lenti ORF clone of Crbn (Myc-DDK-tagged) - Mouse cereblon (cDNA clone MGC:103113 IMAGE:30443656) |
CNY 4,370.00 |
|
| MR206875L4 | Lenti ORF clone of Crbn (mGFP-tagged) - Mouse cereblon (cDNA clone MGC:103113 IMAGE:30443656) |
CNY 4,370.00 |
