Slc35b2 (BC025875) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1110003M08Rik; AA407792; AI842275; PAPST1; Slc35b1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206881 representing BC025875 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGCCAGATGGTGGGCAGTAGTGGTACTCGCCACACTCCCTTCCTTGGGAGCAGGTGGAGAGTCAC CCGAAGCCCCTCCGCAGTCCTGGACACAGCTGTGGCTCTTCCGCTTCTTGTTGAATGTAGCGGGCTATGC CAGCTTTATGGTACCTGGCTACCTCCTGGTGCAGTACTTAAGACGGAAGAACTACCTGGAGACAGGCAGG GGTCTCTGCTTCCCCCTGGTGAAAGCCTGTGTGTTTGGCAATGAGCCCAAGGCTCCTGATGAGGTTCTCC TGGCTCCGCGGACAGAGACAGCGGAATCCACCCCGTCTTGGCAGGTCCTGAAGCTGGTCTTCTGTGCCTC GGGTCTCCAGGTGTCCTATCTGACTTGGGGCATACTGCAGGAAAGAGTGATGACTGGCAGCTACGGGGCC ACAGCCACATCACCAGGAGAGCATTTCACAGACTCCCAGTTTCTGGTGCTGATGAACCGTGTGCTGGCGC TGGTTGTGGCAGGCCTCTACTGTGTCCTGCGCAAGCAGCCCCGTCATGGTGCACCCATGTACCGGTACTC CTTTGCCAGTCTGTCAAATGTGCTTAGCAGCTGGTGCCAGTATGAAGCACTTAAGTTCGTCAGCTTCCCT ACCCAGGTGCTGGCGAAGGCCTCCAAGGTGATCCCTGTCATGATGATGGGAAAGCTGGTGTCCCGGCGCA GCTATGAACACTGGGAATACCTGACTGCCGGCCTCATCTCCATTGGAGTGAGCATGTTTCTTCTATCCAG TGGACCAGAGCCTAGAAGCTCTCCAGCCACCACACTCTCTGGCTTGGTCCTACTGGCAGGCTATATTGCT TTCGACAGCTTCACCTCAAATTGGCAGGATGCCCTGTTTGCCTATAAGATGTCATCGGTGCAGATGATGT TTGGGGTCAATTTATTCTCCTGTCTTTTCACAGTAGGCTCACTACTGGAACAGGGGGCCCTACTGGAGGG GGCACGCTTCATGGGGCGGCACAGTGAGTTTGCGCTCCATGCTCTCCTCCTCTCCATCTGCTCCGCCTTT GGGCAGCTCTTCATCTTCTACACCATTGGACAATTTGGAGCTGCTGTCTTCACTATCATCATGACTCTAC GCCAGGCTATTGCCATCCTCCTCTCCTGCCTCCTCTATGGCCATACTGTCACTGTGGTGGGGGGACTGGG AGTAGCTGTGGTCTTCACTGCCCTCCTACTCAGAGTCTATGCCCGGGGCCGGAAGCAGCGGGGAAAGAAG GCTGTGCCCACTGAGCCCCCAGTACAGAAGGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206881 representing BC025875 Red=Cloning site Green=Tags(s) MDARWWAVVVLATLPSLGAGGESPEAPPQSWTQLWLFRFLLNVAGYASFMVPGYLLVQYLRRKNYLETGR GLCFPLVKACVFGNEPKAPDEVLLAPRTETAESTPSWQVLKLVFCASGLQVSYLTWGILQERVMTGSYGA TATSPGEHFTDSQFLVLMNRVLALVVAGLYCVLRKQPRHGAPMYRYSFASLSNVLSSWCQYEALKFVSFP TQVLAKASKVIPVMMMGKLVSRRSYEHWEYLTAGLISIGVSMFLLSSGPEPRSSPATTLSGLVLLAGYIA FDSFTSNWQDALFAYKMSSVQMMFGVNLFSCLFTVGSLLEQGALLEGARFMGRHSEFALHALLLSICSAF GQLFIFYTIGQFGAAVFTIIMTLRQAIAILLSCLLYGHTVTVVGGLGVAVVFTALLLRVYARGRKQRGKK AVPTEPPVQKV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC025875
ORF Size	1295 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC025875, AAH25875
RefSeq Size	1850 bp
RefSeq ORF	1295 bp
Locus ID	73836
Gene Summary	Mediates the transport of adenosine 3'-phospho 5'-phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl donor for sulfation events that take place in the Golgi (By similarity).[UniProtKB/Swiss-Prot Function]

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