Fbxo9 (BC020074) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	9030401P18Rik; AA986398
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG206949 representing BC020074 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAAGCGGAGGAAGATTGTCATTCTGATGCTGACAGAGTAGGCGATGAAGGAAATGAGAGTCCGG CTGAGAGAGACCTGCAGGCGCAGCTCCAGATGTTCAGAGCTCAGTGGATGTTTGAACTGACCCCAGGTGT AGGCTCCAGTCATGGAGAAACTCGGCCTTGCAGAGCAGGAAGAAGCTCTATGCTGAAAGCAGCTGCAGAC ACCAAAGGACGACAGGAACTGGCAAAGGAAGAAAAGGCTCGAGAACTCTTCCTGCAGGCAGTGGAGGAAG AACAAAATGGAGCTCTCTATGAAGCCATCAAGTTCTACCGTAGGGCGATGCAGCTGGTGCCAGACATTGA GTTCAAGATCACTTACACCCGGTCTCCAGACGGCGACGGCGTTGGGAGCGGCTACATCGAAGAGAACGAG GACGCCAGCAAGATGGCCGATCTCCTGTCGTACTTCCAGCAGCAGCTCACGTTGCAGGAGTCTGTGCTCA AACTCTGTCAGCCTGAGCTTGAGACCAGTCAGACTCACATATCAGTCCTGCCTATGGAGGTGCTGATGTA CATCTTCCGATGGGTGGTATCAAGTGACTTGGACCTCAGATCGTTAGAGCAGTTGTCACTGGTGTGCAGA GGATTCTATATCTGTGCCAGAGACCCTGAAATCTGGCGTCTGGCTTGCTTGAAAGTGTGGGGCAGAAGCT GCATGAAGCTCGTTCCATACGCGTCCTGGAGAGAGATGTTTCTAGAACGGCCCCGAGTTCGGTTTGATGG AGTGTACATCAGTAAAACCACGTATATTCGCCAGGGAGAGCAGTCACTTGATGGTTTCTACAGGGCGTGG CACCAAGTGGAATATTACAGATACATGAGATTCTTTCCTGATGGCCATGTGATGATGTTAACCACCCCCG AGGAGCCTCCGTCCATCGTTCCCCGGTTAAGAACCAGGAACACCAGAACGGATGCAATTCTGCTGGGTCA TTACCGCTTGTCACAAGATGCAGACAATCAGACCAAAGTCTTTGCTGTAATAACTAAGAAAAAAGAAGAA AAGCCACTTGACCATAAATACAGGTATTTTCGTCGTGTTCCTGTCCAAGAGGCAGATCACAGCTTCCATG TGGGACTGCAGCTGTGCTCCAGTGGCCACCAGAGGTTCAACAAACTCATCTGGATCCACCACTCTTGTCA CATCACTTACAAAGCAACTGGTGAGACTGCAGTGAGTGCTTTTGAGATTGACAAGATGTACACGCCCCTG TTGTTCGCCAGAGTGAGGAGCTACACTGCCTTCTCGGAAAGGCCTCTG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG206949 representing BC020074 Red=Cloning site Green=Tags(s) MAEAEEDCHSDADRVGDEGNESPAERDLQAQLQMFRAQWMFELTPGVGSSHGETRPCRAGRSSMLKAAAD TKGRQELAKEEKARELFLQAVEEEQNGALYEAIKFYRRAMQLVPDIEFKITYTRSPDGDGVGSGYIEENE DASKMADLLSYFQQQLTLQESVLKLCQPELETSQTHISVLPMEVLMYIFRWVVSSDLDLRSLEQLSLVCR GFYICARDPEIWRLACLKVWGRSCMKLVPYASWREMFLERPRVRFDGVYISKTTYIRQGEQSLDGFYRAW HQVEYYRYMRFFPDGHVMMLTTPEEPPSIVPRLRTRNTRTDAILLGHYRLSQDADNQTKVFAVITKKKEE KPLDHKYRYFRRVPVQEADHSFHVGLQLCSSGHQRFNKLIWIHHSCHITYKATGETAVSAFEIDKMYTPL LFARVRSYTAFSERPL SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	BC020074
ORF Size	1310 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC020074, AAH20074
RefSeq Size	2138 bp
RefSeq ORF	1310 bp
Locus ID	71538
Gene Summary	Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of TTI1 and TELO2 in a CK2-dependent manner, thereby directly regulating mTOR signaling. SCF(FBXO9) recognizes and binds mTORC1-bound TTI1 and TELO2 when they are phosphorylated by CK2 following growth factor deprivation, leading to their degradation. In contrast, the SCF(FBXO9) does not mediate ubiquitination of TTI1 and TELO2 when they are part of the mTORC2 complex. As a consequence, mTORC1 is inactivated to restrain cell growth and protein translation, while mTORC2 is activated due to the relief of feedback inhibition by mTORC1 (By similarity).[UniProtKB/Swiss-Prot Function]

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