Map2k5 (BC028260) Mouse Tagged ORF Clone

CAT#: MG206994

  • TrueORF®

Map2k5 (tGFP-tagged) - Mouse mitogen activated protein kinase kinase 5 (cDNA clone MGC:40633 IMAGE:5067818)

ORF Plasmid: DDK tGFP



  "BC028260" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,760.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Map2k5 Antibody - N-terminal region
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms MEK5, Mapkk5, Prkmk5
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG206994 representing BC028260
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTGGCTGGCCCTTGGCCCCTTCTGTGCCATGGAGAACCAGGTGCTGGTGATTCGGATCAAGATTC
CAAATAGTGGCGCGGTGGACTGGACCGTGCACTCCGGGCCGCAGTTACTCTTCAGGGATGTTCTGGATGT
GATAGGCCAGGTTCTGCCTGAAGCGACGACGACAGCGTTTGAATATGAAGATGAAGATGGTGATAGGATT
ACAGTAAGAAGCGATGAAGAGATGAAGGCAATGCTGTCCTACTATTATTCCACAGTAATGGAACAGCAAG
TAAATGGCCAGCTAATAGAGCCGCTGCAGATATTTCCAAGAGCCTGCAAGCCTCCTGGGGAACGGAACAT
ACATGGCCTGAAGGTGAATACACGGGCTGGGCCATCTCAACACACCAGCCCTGTGGTCTCAGATTCACTT
CCAAGCAATAGCTTGAAGAAGTCCTCAGCTGAACTGAGAAAGATACTGGCCAACGGCCAGATGAATGAAC
AAGACATACGGTATCGAGACACCCTTGGTCATGGCAACGGAGGCACAGTCTACAAAGCACATCATGTCCC
AAGTGGGAAAATTTTAGCTGTAAAGGTTATTCTGTTAGACATTACACTGGAGCTTCAGAAGCAGATCATG
TCTGAGCTGGAAATCCTTTATAAGTGTGACTCATCGTATATCATAGGATTTTACGGGGCATTTTTCGTAG
AAAACAGGATTTCAATTTGTACAGAATTCATGGATGGGGGGTCTTTGGATGTATATAGGAAAATTCCAGA
GCACGTCCTTGGAAGAATTGCAGTTGCAGTTGTTAAAGGCCTTACCTATCTGTGGAGTTTAAAGATTTTA
CACAGAGATGTGAAGCCCTCCAACATGCTTGTAAACACAGGCGGACAGGTCAAGCTGTGTGACTTTGGCG
TGAGCACACAGCTGGTGAATTCTATAGCCAAGACGTATGTTGGAACAAATGCTTACATGGCACCAGAAAG
AATTTCAGGAGAGCAGTATGGGATCCATTCTGACGTGTGGAGCTTAGGAATCTCTTTCATGGAGCTTGCT
CTTGGGAGGTTTCCATATCCTCAGCCTCTCCAGCTTCTGCAGTGCATTGTTGATGAGGATTCGCCGGTCC
TTCCTCTTGGAGAGTTCTCGGAGCCGTTTGTACATTTCATCACTCAGTGCATGAGGAAGCAGCCCAAGGA
GAGACCGGCGCCTGAGGAGCTGATGGGTCACCCATTCATCGTGCAGTTCAATGATGGAAACTCCACTGTG
GTGTCCATGTGGGTGTGCCGAGCTCTAGAGGAGAGGCGGAGCCAGCAGGGACCCCCG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG206994 representing BC028260
Red=Cloning site Green=Tags(s)

MLWLALGPFCAMENQVLVIRIKIPNSGAVDWTVHSGPQLLFRDVLDVIGQVLPEATTTAFEYEDEDGDRI
TVRSDEEMKAMLSYYYSTVMEQQVNGQLIEPLQIFPRACKPPGERNIHGLKVNTRAGPSQHTSPVVSDSL
PSNSLKKSSAELRKILANGQMNEQDIRYRDTLGHGNGGTVYKAHHVPSGKILAVKVILLDITLELQKQIM
SELEILYKCDSSYIIGFYGAFFVENRISICTEFMDGGSLDVYRKIPEHVLGRIAVAVVKGLTYLWSLKIL
HRDVKPSNMLVNTGGQVKLCDFGVSTQLVNSIAKTYVGTNAYMAPERISGEQYGIHSDVWSLGISFMELA
LGRFPYPQPLQLLQCIVDEDSPVLPLGEFSEPFVHFITQCMRKQPKERPAPEELMGHPFIVQFNDGNSTV
VSMWVCRALEERRSQQGPP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC028260
ORF Size 1319 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC028260, AAH28260
RefSeq Size 2149 bp
RefSeq ORF 1319 bp
Locus ID 23938
Gene Summary Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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