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Map2k5 (BC028260) Mouse Tagged ORF Clone
CAT＃: MR206994
- TrueORF®
Map2k5 (Myc-DDK-tagged) - Mouse mitogen activated protein kinase kinase 5 (cDNA clone MGC:40633 IMAGE:5067818)
ORF Plasmid: tGFP
"BC028260" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MEK5, Mapkk5, Prkmk5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206994 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTGGCTGGCCCTTGGCCCCTTCTGTGCCATGGAGAACCAGGTGCTGGTGATTCGGATCAAGATTC CAAATAGTGGCGCGGTGGACTGGACCGTGCACTCCGGGCCGCAGTTACTCTTCAGGGATGTTCTGGATGT GATAGGCCAGGTTCTGCCTGAAGCGACGACGACAGCGTTTGAATATGAAGATGAAGATGGTGATAGGATT ACAGTAAGAAGCGATGAAGAGATGAAGGCAATGCTGTCCTACTATTATTCCACAGTAATGGAACAGCAAG TAAATGGCCAGCTAATAGAGCCGCTGCAGATATTTCCAAGAGCCTGCAAGCCTCCTGGGGAACGGAACAT ACATGGCCTGAAGGTGAATACACGGGCTGGGCCATCTCAACACACCAGCCCTGTGGTCTCAGATTCACTT CCAAGCAATAGCTTGAAGAAGTCCTCAGCTGAACTGAGAAAGATACTGGCCAACGGCCAGATGAATGAAC AAGACATACGGTATCGAGACACCCTTGGTCATGGCAACGGAGGCACAGTCTACAAAGCACATCATGTCCC AAGTGGGAAAATTTTAGCTGTAAAGGTTATTCTGTTAGACATTACACTGGAGCTTCAGAAGCAGATCATG TCTGAGCTGGAAATCCTTTATAAGTGTGACTCATCGTATATCATAGGATTTTACGGGGCATTTTTCGTAG AAAACAGGATTTCAATTTGTACAGAATTCATGGATGGGGGGTCTTTGGATGTATATAGGAAAATTCCAGA GCACGTCCTTGGAAGAATTGCAGTTGCAGTTGTTAAAGGCCTTACCTATCTGTGGAGTTTAAAGATTTTA CACAGAGATGTGAAGCCCTCCAACATGCTTGTAAACACAGGCGGACAGGTCAAGCTGTGTGACTTTGGCG TGAGCACACAGCTGGTGAATTCTATAGCCAAGACGTATGTTGGAACAAATGCTTACATGGCACCAGAAAG AATTTCAGGAGAGCAGTATGGGATCCATTCTGACGTGTGGAGCTTAGGAATCTCTTTCATGGAGCTTGCT CTTGGGAGGTTTCCATATCCTCAGCCTCTCCAGCTTCTGCAGTGCATTGTTGATGAGGATTCGCCGGTCC TTCCTCTTGGAGAGTTCTCGGAGCCGTTTGTACATTTCATCACTCAGTGCATGAGGAAGCAGCCCAAGGA GAGACCGGCGCCTGAGGAGCTGATGGGTCACCCATTCATCGTGCAGTTCAATGATGGAAACTCCACTGTG GTGTCCATGTGGGTGTGCCGAGCTCTAGAGGAGAGGCGGAGCCAGCAGGGACCCCCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206994 protein sequence
Red=Cloning site Green=Tags(s) MLWLALGPFCAMENQVLVIRIKIPNSGAVDWTVHSGPQLLFRDVLDVIGQVLPEATTTAFEYEDEDGDRI TVRSDEEMKAMLSYYYSTVMEQQVNGQLIEPLQIFPRACKPPGERNIHGLKVNTRAGPSQHTSPVVSDSL PSNSLKKSSAELRKILANGQMNEQDIRYRDTLGHGNGGTVYKAHHVPSGKILAVKVILLDITLELQKQIM SELEILYKCDSSYIIGFYGAFFVENRISICTEFMDGGSLDVYRKIPEHVLGRIAVAVVKGLTYLWSLKIL HRDVKPSNMLVNTGGQVKLCDFGVSTQLVNSIAKTYVGTNAYMAPERISGEQYGIHSDVWSLGISFMELA LGRFPYPQPLQLLQCIVDEDSPVLPLGEFSEPFVHFITQCMRKQPKERPAPEELMGHPFIVQFNDGNSTV VSMWVCRALEERRSQQGPP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC028260 |
| ORF Size | 1317 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC028260, AAH28260 |
| RefSeq Size | 2149 bp |
| RefSeq ORF | 1319 bp |
| Locus ID | 23938 |
| MW | 49.1 kDa |
| Gene Summary | Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217771 | Map2k5 (untagged) - Mouse mitogen activated protein kinase kinase 5 (cDNA clone MGC:40633 IMAGE:5067818), (10ug) |
CNY 6,940.00 |
|
| MG206994 | Map2k5 (tGFP-tagged) - Mouse mitogen activated protein kinase kinase 5 (cDNA clone MGC:40633 IMAGE:5067818) |
CNY 2,760.00 |
|
| MR206994L3 | Lenti ORF clone of Map2k5 (Myc-DDK-tagged) - Mouse mitogen activated protein kinase kinase 5 (cDNA clone MGC:40633 IMAGE:5067818) |
CNY 4,370.00 |
|
| MR206994L4 | Lenti ORF clone of Map2k5 (mGFP-tagged) - Mouse mitogen activated protein kinase kinase 5 (cDNA clone MGC:40633 IMAGE:5067818) |
CNY 4,370.00 |
