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E2f2 (NM_177733) Mouse Tagged ORF Clone
CAT＃: MG207069
- TrueORF®
E2f2 (tGFP-tagged) - Mouse E2F transcription factor 2 (E2f2)
ORF Plasmid: DDK
"NM_177733" in other vectors (4)
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CNY 4,280.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | E2F-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207069 representing NM_177733
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCGCGCGCCGCGGACCCTGGCTCCGGCCACGGCGCAACCTACAAAGAGCTTGCCGGCGCTGAACC CCACCGAGCTGTGGCCTTCGGGTCTGAGCAGCCCCCAGCTCTGCCCGGCCACCACCGCCACCACCTACTA CACTTCGCTTTACACGCAGACGGTGCCTTCCTCTGTGGCGCTGGGCACCTGCCTCGACGCCACTCCCCAC GGACCCGAGGGCCAAATTGTGCGATGTGCACCCGCAGGCCGGCTGCCGGCCAAAAGGAAGTTGGACCTGG AGGGCATTGGGAGGCCTACGGTCCCTGAATTCCGGACCCCCAAGGGGAAGTGCATCCGCGTGGATGGTTT GCCAAGCCCCAAAACCCCCAAGTCTCCTGGGGAGAAGACACGCTATGACACGTCGCTGGGGCTCCTGACC AAGAAGTTCATTTACCTCCTGAGCGAGTCGGAGGATGGAGTCCTGGACCTGAACTGGGCAGCCGAGGTGC TGGATGTGCAAAAGCGGCGCATCTATGACATCACCAACGTGCTGGAGGGTATCCAGCTCATCCGCAAGAA GTCCAAAAACAACATCCAGTGGGTAGGCAGGGAACTATTTGAAGACCCCACCCGACCCTCCAGGCAGCAG CAGTTGGGGCAGGAGCTGAAGGAGCTGATGAATGCCGAGCAGACCTTGGACCAGCTCATTCAGAGTTGCT CCCTGAGCTTCAAGCACCTGACCGAAGATAATGCCAACAAGAAACTGGCCTATGTGACCTACCAGGATAT CCGTGCCGTAGGCAACTTCAAGGAGCAGACAGTGATTGCGGTCAAGGCCCCACCACAGACAAGATTGGAA GTGCCGGACAGGGCCGAGGAGAACCTGCAGATTTATCTAAAGAGTACCCAAGGCCCCATTGAAGTCTACC TGTGCCCAGAGGAGGGGCAGGAGCCAGACAGTCCTGCCAAGGAGGCGCTCCCCTCCACCTCTGCCCTCAG CCCCATTCCTGACTGCGCTCAGCCGGGCTGCAGCACTGACTCTGGGATCGCAGAGACCATAGAGCCTTCA GTACTGATACCCCAGCCGATACCACCGCCTCCTCCACCACCACTGCCGCCAGCCCCATCCCTCGTCCCCT TGGAAGCCACTGACAACATGCTGGAGCTGTCACACCCTCTTCTACAACAGACTGAGGACCAGTTCCTGTC CCCAATCCTGGCGGCCAACTCCCCCCTGATCAGCTTCTCCCCGCCCTTGGACCAGGACGAATACCTGTGG GGCATGGACGAGGGGGAAGGCATCAGTGACCTCTTCGACTCCTATGACCTTGGGGACCTGTTGATTAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207069 representing NM_177733
Red=Cloning site Green=Tags(s) MLRAPRTLAPATAQPTKSLPALNPTELWPSGLSSPQLCPATTATTYYTSLYTQTVPSSVALGTCLDATPH GPEGQIVRCAPAGRLPAKRKLDLEGIGRPTVPEFRTPKGKCIRVDGLPSPKTPKSPGEKTRYDTSLGLLT KKFIYLLSESEDGVLDLNWAAEVLDVQKRRIYDITNVLEGIQLIRKKSKNNIQWVGRELFEDPTRPSRQQ QLGQELKELMNAEQTLDQLIQSCSLSFKHLTEDNANKKLAYVTYQDIRAVGNFKEQTVIAVKAPPQTRLE VPDRAEENLQIYLKSTQGPIEVYLCPEEGQEPDSPAKEALPSTSALSPIPDCAQPGCSTDSGIAETIEPS VLIPQPIPPPPPPPLPPAPSLVPLEATDNMLELSHPLLQQTEDQFLSPILAANSPLISFSPPLDQDEYLW GMDEGEGISDLFDSYDLGDLLIN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177733 |
| ORF Size | 1329 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177733.7, NP_808401.1 |
| RefSeq Size | 4746 bp |
| RefSeq ORF | 1332 bp |
| Locus ID | 242705 |
| UniProt ID | P56931 |
| Gene Summary | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202421 | E2f2 (untagged) - Mouse E2F transcription factor 2 (E2f2), (10ug) |
CNY 3,656.00 |
|
| MR207069 | E2f2 (Myc-DDK-tagged) - Mouse E2F transcription factor 2 (E2f2) |
CNY 3,656.00 |
|
| MR207069L3 | Lenti ORF clone of E2f2 (Myc-DDK-tagged) - Mouse E2F transcription factor 2 (E2f2) |
CNY 5,800.00 |
|
| MR207069L4 | Lenti ORF clone of E2f2 (mGFP-tagged) - Mouse E2F transcription factor 2 (E2f2) |
CNY 5,800.00 |
