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Tuba1b (BC002219) Mouse Tagged ORF Clone
CAT＃: MG207204
- TrueORF®
Tuba1b (tGFP-tagged) - Mouse tubulin, alpha 1B (cDNA clone MGC:7476 IMAGE:3490568)
ORF Plasmid: DDK
"BC002219" in other vectors (3)
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CNY 2,850.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Tuba2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207204 representing BC002219
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGTGAGTGCATCTCCATCCACGTTGGCCAGGCTGGTGTCCAGATCGGCAATGCCTGCTGGGAGCTCT ACTGCCTGGAACATGGCATCCAGCCTGATGGCCAGATGCCAAGTGACAAGACCATTGGGGGAGGAGATGA CTCCTTCAACACCTTCTTCAGTGAGACAGGAGCTGGCAAGCATGTGCCCCGGGCAGTGTTCGTAGACCTG GAACCCACGGTCATCGATGAAGTTCGCACTGGCACCTACCGCCAGCTCTTCCATCCTGAGCAGCTCATCA CAGGCAAGGAGGATGCTGCCAATAACTATGCCCGTGGCCACTACACCATTGGCAAGGAGATCATTGACCT TGTCCTGGACAGGATTCGCAAGCTGGCTGACCAGTGCACGGGTCTCCAGGGCTTCTTGGTTTTCCACAGC TTTGGCGGGGGAACTGGCTCTGGCTTCACCTCCCTGCTGATGGAGCGGCTCTCTGTGGATTACGGAAAGA AGTCCAAGCTGGAGTTCTCCATTTACCCAGCCCCCCAGGTTTCCACTGCTGTGGTTGAGCCCTACAATTC CATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCATCTAT GACATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACCAACCTTAACCGCCTTATTAGCCAGA TTGTGTCTTCCATCACTGCTTCCCTCAGATTTGATGGGGCCCTGAATGTTGATCTGACAGAATTCCAGAC CAACCTGGTACCCTACCCTCGCATCCACTTCCCTCTGGCCACTTATGCCCCTGTCATCTCTGCTGAGAAA GCCTACCATGAGCAGCTTTCTGTAGCAGAGATCACCAATGCCTGCTTTGAGCCAGCCAACCAGATGGTGA AATGTGACCCTCGCCATGGTAAATACATGGCTTGCTGCCTGCTATACCGTGGTGATGTGGTTCCCAAAGA TGTCAATGCTGCCATTGCCACCATCAAGACCAAGCGCAGCATCCAGTTTGTAGACTGGTGCCCCACTGGC TTCAAGGTTGGCATTAATTACCAGCCTCCCACTGTGGTACCCGGTGGTGACCTGGCCAAGGTGCAGAGAG CTGTGTGCATGCTGAGCAACACCACAGCCATTGCTGAGGCCTGGGCTCGCCTAGATCACAAGTTTGATCT GATGTATGCCAAGCGTGCCTTTGTGCACTGGTATGTGGGTGAGGGCATGGAGGAGGGTGAGTTCTCTGAG GCCCGTGAGGACATGGCTGCCCTAGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTGGAAGGCGAGG GGGAGGAAGAAGGAGAGGAGTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207204 representing BC002219
Red=Cloning site Green=Tags(s) MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDL EPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHS FGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIY DICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK AYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTG FKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE AREDMAALEKDYEEVGVDSVEGEGEEEGEEY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC002219 |
| ORF Size | 1355 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC002219, AAH02219 |
| RefSeq Size | 1616 bp |
| RefSeq ORF | 1355 bp |
| Locus ID | 22143 |
| Gene Summary | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR207204 | Tuba1b (Myc-DDK-tagged) - Mouse tubulin, alpha 1B (cDNA clone MGC:7476 IMAGE:3490568) |
CNY 3,656.00 |
|
| MR207204L3 | Lenti ORF clone of Tuba1b (Myc-DDK-tagged) - Mouse tubulin, alpha 1B (cDNA clone MGC:7476 IMAGE:3490568) |
CNY 4,470.00 |
|
| MR207204L4 | Lenti ORF clone of Tuba1b (mGFP-tagged) - Mouse tubulin, alpha 1B (cDNA clone MGC:7476 IMAGE:3490568) |
CNY 4,470.00 |
