Sil1 (BC016119) Mouse Tagged ORF Clone
CAT#: MG207424
- TrueORF®
Sil1 (tGFP-tagged) - Mouse endoplasmic reticulum chaperone SIL1 homolog (S
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CNY 3,710.00
货期*
4周
规格
经常一起买 (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 1810057E01Rik; AI042831; wz |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG207424 representing BC016119
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCTCAGCACCTGCCTTCAACTAGGATGGCCTCTCCAGGAATGCTGCTGGGGCTGCTGTTGACTT CCTGTTTAACTCTCTGCCTCAGCTGTCAGAACTCAAATAATTTTGCACTCACCAACCCAGAGAAGAGCAT CCACCAGGAATCAGATACAAAGGAGACCAGGGAAGAGGAGGAGCTAGACACCGAGATCCTGGAGGTGTTC CACCCAACTCAAGAGTGGCAGACCCTTCAACCAGGTCAGGCTGTTCCTGCAGGATCCCATGTGCGAATGA ACCTACAGACTGGAGTAAACGAGGTGAAGCTCCAACAAGAAGACAAATTTCAAAATAATCTGAAAGGATT TAAAAGAGGCAGAAGGCTGGACATCAACGCCAACACATACACATCTCAGGACCTCAAGAGTGCGCTGGCC AAATTCAAGGAGGGGACCGAGATGGAGAATTCAAAGGATGAACTGGCGAGACAGGCCACAGTCAAGCAGC TCTTCCGCCCCATTGAGGAGCTGAAGAAGGAATTTGATGAGCTGAACGTTGTTCTTGAGACTGACATGCA GATTATGGTTCGGCTGATAAACAAGTTCAACAGCTCCAGCTCCAGCCTAGAAGAGAAGGTTGCTGCGCTC TTTGATCTTGAGTACTATGTCCATCAGATGGATAACGCACAGGACCTGCTGTCCTTTGGTGGCCTTCAAG TGGTGATTAATGGGCTCAACAGCACAGAACCCCTGGTGAAGGAGTATGCTGCTTTTGTGCTGGGGGCCGC CTTTTCCAGCAATCCGAAGGTCCAGGTGGAAGCCATCGAAGGGGGAGCCCTGCAGAAGCTGCTGGTCATC CTGGCCACAAACCAGCCTCTCCCCGCCAAGAAGAAGGTCCTGTTTGCACTGTGCTCCCTGCTGCGCCACT TCCCCTATGCCCAGCAGCAGTTCCTGAAGCTCGGGGGCCTGCAGGTCCTCAGGAGCCTGGTACAGGAGAA GAGTGCGAAGGTACTGGCCGTGCGTGTGGTCACCCTGCTCTATGACCTGGTCACTGAGAAGATGTTTGCT GAGGAGGAGGCAGAGTTGACCCAGGATTCATCCCCAGAGAAGCTGCAGCAGTATCGCCAGGTGCAGCTCC TGCCAGGCCTGCAGGAACAGGGTTGGTGTGAGATCACCGCCCAGCTCCTGGCCCTTCCAGAGCATGATGC TCGGGAGAAGGTGCTGCAGACACTGGGTGCCCTCCTGACCACCTGCCGGGATCGCTACCGTCAGGACCTC CAGTTGAGCAGGACACTAGGGCGTCTCCAAGCAGAGTACCAAGCCCTGGCCTCCCTGGAGCTCCAAGAAG GCGAAGATGATGGCTACTTCCGGGAACTGCTGGCCTCCATCAACAGCTTGATGAAGGAACTAAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207424 representing BC016119
Red=Cloning site Green=Tags(s) MAPQHLPSTRMASPGMLLGLLLTSCLTLCLSCQNSNNFALTNPEKSIHQESDTKETREEEELDTEILEVF HPTQEWQTLQPGQAVPAGSHVRMNLQTGVNEVKLQQEDKFQNNLKGFKRGRRLDINANTYTSQDLKSALA KFKEGTEMENSKDELARQATVKQLFRPIEELKKEFDELNVVLETDMQIMVRLINKFNSSSSSLEEKVAAL FDLEYYVHQMDNAQDLLSFGGLQVVINGLNSTEPLVKEYAAFVLGAAFSSNPKVQVEAIEGGALQKLLVI LATNQPLPAKKKVLFALCSLLRHFPYAQQQFLKLGGLQVLRSLVQEKSAKVLAVRVVTLLYDLVTEKMFA EEEAELTQDSSPEKLQQYRQVQLLPGLQEQGWCEITAQLLALPEHDAREKVLQTLGALLTTCRDRYRQDL QLSRTLGRLQAEYQALASLELQEGEDDGYFRELLASINSLMKELR TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC016119 |
ORF Size | 1397 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC016119, AAH16119 |
RefSeq Size | 1689 bp |
RefSeq ORF | 1397 bp |
Locus ID | 81500 |
Gene Summary | Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5 (By similarity).[UniProtKB/Swiss-Prot Function] |
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