Cap1 (BC005446) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207594 representing BC005446 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGACATGCAAAATCTTGTAGAAAGATTGGAGAGGGCAGTGGGCCGCCTGGAGGCAGTGTCACATA CTTCAGACATGCACTGTGGATATGGAGACAGCCCTTCAAAAGGAGCAGTTCCATATGTGCAAGCATTTGA CTCGCTGCTTGCCAATCCCGTGGCAGAGTACTTGAAGATGAGTAAGGAGATCGGGGGAGATGTGCAGAAA CACGCGGAGATGGTCCACACAGGCCTGAAGTTGGAGCGAGCTCTCCTGGCTACAGCTTCTCAGTGCCAGC AGCCAGCTGGTAATAAACTTTCTGATTTGTTGGCACCTATCTCGGAGCAGATCCAAGAAGTTATAACCTT CCGGGAGAAGAACCGAGGCAGCAAGTTTTTTAATCATCTATCTGCTGTCAGTGAAAGCATCCAGGCCTTG GGCTGGGTGGCTCTGGCTGCGAAACCTGGCCCCTTTGTGAAAGAGATGAATGACGCGGCCATGTTTTACA CAAATCGTGTCCTCAAGGAGTACAGAGATGTGGATAAGAAGCATGTGGACTGGGTCAGAGCTTACTTGAG TATATGGACGGAGCTGCAGGCTTACATCAAGGAGTTTCATACTACTGGCCTGGCCTGGAGCAAGACGGGG CCTGTGGCAAAAGAACTGAGTGGATTGCCATCTGGACCCTCTGTGGGATCAGGCCCACCTCCTCCCCCAC CGGGCCCGCCTCCTCCCCCAATTCCTACCAGTTCTGGTTCTGACGACTCTGCATCACGCTCAGCACTGTT TGCACAGATTAATCAGGGGGAAAGCATCACACATGCCCTGAAACATGTATCTGATGACATGAAGACTCAC AAGAACCCTGCCCTGAAAGCTCAGAGCGGTCCAGTTCGGAGTGGCCCCAAACCATTCTCTGCACCTAAAC CCCAAACTAGCCCCTCCCCCAAACCAGCCACAAAGAAGGAACCAGCTCTGCTGGAACTGGAAGGCAAGAA ATGGAGAGTGGAAAACCAGGAGAATGTTTCTAACCTGGTGATTGATGACACTGAGCTGAAGCAGGTGGCT TACATCTACAAGTGTGTCAACACAACATTGCAAATCAAGGGCAAAATTAACTCCATTACAGTAGATAACT GTAAGAAGCTTGGCCTGGTGTTTGATGACGTGGTGGGCATTGTGGAGATAATCAATAGTAGGGATGTCAA AGTTCAGGTGATGGGAAAAGTGCCAACCATTTCCATTAACAAAACAGATGGCTGCCATGCTTACCTGAGC AAGAACTCCCTGGACTGTGAGATAGTCAGTGCCAAATCTTCTGAGATGAATGTCCTCATTCCTACCGAAG GCGGTGATTTTAACGAGTTCCCAGTCCCCGAGCAGTTCAAGACCCTGTGGAACGGACAGAAGTTGGTCAC CACAGTGACAGAAATCGCTGGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207594 representing BC005446 Red=Cloning site Green=Tags(s) MADMQNLVERLERAVGRLEAVSHTSDMHCGYGDSPSKGAVPYVQAFDSLLANPVAEYLKMSKEIGGDVQK HAEMVHTGLKLERALLATASQCQQPAGNKLSDLLAPISEQIQEVITFREKNRGSKFFNHLSAVSESIQAL GWVALAAKPGPFVKEMNDAAMFYTNRVLKEYRDVDKKHVDWVRAYLSIWTELQAYIKEFHTTGLAWSKTG PVAKELSGLPSGPSVGSGPPPPPPGPPPPPIPTSSGSDDSASRSALFAQINQGESITHALKHVSDDMKTH KNPALKAQSGPVRSGPKPFSAPKPQTSPSPKPATKKEPALLELEGKKWRVENQENVSNLVIDDTELKQVA YIYKCVNTTLQIKGKINSITVDNCKKLGLVFDDVVGIVEIINSRDVKVQVMGKVPTISINKTDGCHAYLS KNSLDCEIVSAKSSEMNVLIPTEGGDFNEFPVPEQFKTLWNGQKLVTTVTEIAG TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC005446
ORF Size	1424 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC005446, AAH05446
RefSeq Size	2543 bp
RefSeq ORF	1424 bp
Locus ID	12331
Gene Summary	The product of this gene plays a role in regulating actin dynamics by binding actin monomers and promoting the turnover of actin filaments. Reduced expression of this gene causes a reduction in actin filament turnover rates, causing multiple defects, including an increase in cell size, stress-fiber alterations, and defects in endocytosis and cell motility. A pseudogene of this gene is found on chromosome 14. Alternative splicing results in multiple transcript variants, but does not affect the protein. [provided by RefSeq, Jul 2014]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.