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Cdc23 (BC059013) Mouse Tagged ORF Clone
CAT＃: MG207684
- TrueORF®
Cdc23 (tGFP-tagged) - Mouse CDC23 (cell division cycle 23, yeast, homolog) (cDNA clone MGC:69646 IMAGE:6813730)
ORF Plasmid: DDK
"BC059013" in other vectors (4)
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CNY 5,230.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 6030435O18; D18Ertd243e |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207684 representing BC059013
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATTCCAGATACCTGTCTGGAGAGAAGAAGAAGGATGACGAAACAGTTGATAGCCTAGGACCCCTAG AGAAGGGACAAGTCAAAAACGAGGCTCTCAGAGAACTGAGAGTGGAACTCAGCCGGAAGCACCAGGCTCG GGGACTTGATGGATTCGGCCTTTACCTGTATGGGGTGGTGCTTCGGAAACTGGACTTAGTGAAAGAGGCC ATTGATGTGTTTGTGGAGGCTACTCATGTTTTGCCTTTGCACTGGGGAGCCTGGTTAGAGCTGTGTAACC TGATCACAGACAAAGAGATGCTGAAGTTCCTGTCTTTGCCTGACACCTGGATGAAAGAGTTCTTCTTGGC TCACATATACACAGAACTGCAGCTGATAGAGGAGGCCCTGCAGAAGTATCAGCATCTCATTGATGTGGGC TTTTCCAAGAGCTCGTACATTGTTTCCCAAATTGCAGTTGCCTATCACAATATCAGAGATATCGACAAAG CCCTTTCTATTTTCAATGAGCTGAGGAAACAAGATCCTTACAGGATTGAAAATATGGACACATTCTCCAA CCTTCTCTATGTCAGGAGCATGAAGTCTGAATTGAGTTACCTGGCGCATAACCTCTGTGAAATTGATAAA TACCGAGTAGAAACATGCTGTGTAATTGGCAATTATTACAGCTTACGCTCCCAGCATGAGAAAGCAGCTT TGTATTTCCAGAGAGCCCTAAAGTTGAATCCTAGGTACCTTGGGGCCTGGACACTGATGGGGCACGAGTA CATGGAGATGAAGAACACGTCTGCAGCTATTCAGGCTTACCGACATGCCATTGAGGTCAACAAGAGAGAC TACAGAGCTTGGTATGGCCTTGGGCAGACTTACGAAATCCTTAAAATGCCATTTTATTGCCTTTATTATT ATAGACGGGCCCACCAGCTTCGTCCCAATGATTCTCGCATGCTGGTTGCCTTAGGAGAATGTTATGAGAA ACTCAATCAACTAGTGGAAGCCAAAAAGTGTTATTGGAGAGCATATGCCGTGGGAGACGTGGAGAAAATG GCTCTTGTGAAGCTGGCCAAGCTTCATGAACAGTTGACTGAGTCAGAGCAGGCTGCCCAGTGTTACATCA AATACATCCAAGATATCTATTCCTGTGGGGAGACCGTGGAGCACTTGGAGGAGAGCACAGCCTTCCGATA CCTGGCCCAGTACTATTTTAAGTGCAAGCTGTGGGATGAAGCTTCAACTTGTGCCCAAAAATGTTGTGCA TTCAATGATACCCGGGAAGAAGGTAAGGCCTTGCTCCGCCAGATCCTACAGCTGAGGAACCAAGGGGAGA CACCCACCTCTGACACGCCGGGTACCTTTTTCCTCCCTGCCTCACTGTCTGCAAACAACACTCCTACACG CAGAGTTTCTCCACTCAACCTGTCTTCAGTCACACCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207684 representing BC059013
Red=Cloning site Green=Tags(s) MYSRYLSGEKKKDDETVDSLGPLEKGQVKNEALRELRVELSRKHQARGLDGFGLYLYGVVLRKLDLVKEA IDVFVEATHVLPLHWGAWLELCNLITDKEMLKFLSLPDTWMKEFFLAHIYTELQLIEEALQKYQHLIDVG FSKSSYIVSQIAVAYHNIRDIDKALSIFNELRKQDPYRIENMDTFSNLLYVRSMKSELSYLAHNLCEIDK YRVETCCVIGNYYSLRSQHEKAALYFQRALKLNPRYLGAWTLMGHEYMEMKNTSAAIQAYRHAIEVNKRD YRAWYGLGQTYEILKMPFYCLYYYRRAHQLRPNDSRMLVALGECYEKLNQLVEAKKCYWRAYAVGDVEKM ALVKLAKLHEQLTESEQAAQCYIKYIQDIYSCGETVEHLEESTAFRYLAQYYFKCKLWDEASTCAQKCCA FNDTREEGKALLRQILQLRNQGETPTSDTPGTFFLPASLSANNTPTRRVSPLNLSSVTP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC059013 |
| ORF Size | 1437 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC059013, AAH59013 |
| RefSeq Size | 3015 bp |
| RefSeq ORF | 1439 bp |
| Locus ID | 52563 |
| Gene Summary | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218308 | Cdc23 (untagged) - Mouse CDC23 (cell division cycle 23, yeast, homolog) (cDNA clone MGC:69646 IMAGE:6813730), (10ug) |
CNY 3,656.00 |
|
| MR207684 | Cdc23 (Myc-DDK-tagged) - Mouse CDC23 (cell division cycle 23, yeast, homolog) (cDNA clone MGC:69646 IMAGE:6813730) |
CNY 4,750.00 |
|
| MR207684L3 | Lenti ORF clone of Cdc23 (Myc-DDK-tagged) - Mouse CDC23 (cell division cycle 23, yeast, homolog) (cDNA clone MGC:69646 IMAGE:6813730) |
CNY 6,650.00 |
|
| MR207684L4 | Lenti ORF clone of Cdc23 (mGFP-tagged) - Mouse CDC23 (cell division cycle 23, yeast, homolog) (cDNA clone MGC:69646 IMAGE:6813730) |
CNY 6,650.00 |
