Eya3 (NM_010166) Mouse Tagged ORF Clone
CAT#: MG208198
- TrueORF®
Eya3 (tGFP-tagged) - Mouse eyes absent 3 homolog (Drosophila) (Eya3), transcript variant 2
ORF Plasmid: DDK
"NM_010166" in other vectors (4)
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CNY 4,940.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AI844637 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG208198 representing NM_010166
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAAGAGCAAGACCTACCAGAGCAACCGGTGAAAAAAGCCAAGATGCAGGAACCAAGAGAACAGA CTTTAAGTCAAGTAAACAACCCAGATGCCAGTGATGAGAAGCCTGAGACATCCAGCCTTGCGTCAAATCT CAGCATGTCAGAGGAAATTATGACATGCACCGATTACATCCCTCGCTCATCCAATGATTATACCTCACAA ATGTATTCTGCAAAACCTTATGCACACATCCTCTCAGTTCCTGTTTCGGAAACCACTTATCCTGGGCAGA CTCAGTACCAGACACTGCAGCAATCTCAACCCTACGCTGTCTACCCTCAGGCAACCCAAACTTACGGACT ACCTCCTTTCGCTTCAAGCACAAATGCCAGCCTGATACCCACTTCATCTGCAATTGCCAATATTCCAGCA GCAGCTGTGGCCAGCATCTCAAACCAGGATTATCCCACCTATACTATTCTTGGACAGAATCAGTACCAGG CCTGCTACCCCAGTTCCAGCTTTGGAGTCACAGGTCAGACTAACAGTGATGCTGAGACCACAACATTAGC AGCTACAACATACCAGACGGAGAAGCCTAGTGCTATGGTGCCTGCACCAGCCACACAGAGGCTTCCCTCC GACTCCTCTGCAAGCCCACCTTTGTCCCAGACTACACCAAATAAAGATGCTGATGATCAGGCCAGGAAAA ACATGACTGTCAAGAACCGGGGCAAGAGGAAAGCTGATGCCAGCTCTTCCCAGGACAGTGAATTGGAACG GGTATTTCTCTGGGACTTGGACGAAACCATCATCATCTTTCATTCCCTTCTCACTGGATCCTATGCTCAG AAGTATGGAAAGGACCCAACAGTAGTAATTGGCTCAGGTTTAACCATGGAAGAAATGATTTTTGAAGTGG CTGATACACATCTATTTTTCAATGACTTAGAGGAGTGTGACCAGGTGCATGTGGAAGATGTGGCTTCTGA TGACAATGGCCAGGATTTGAGCAACTACAGTTTCTCCACAGATGGTTTCAGTGGTTCAGGAGGCAGTGGT AGCCACGGCTCATCTGTGGGCGTTCAGGGAGGTGTGGACTGGATGAGGAAACTGGCCTTTCGCTACCGAA AAGTGAGGGAAATCTACGACAAGCATAAAAGCAATGTGGGTGGCCTCCTCAGCCCCCAGAGGAAGGAAGC ACTGCAGAGACTCAGAGCAGAGATCGAGGTGCTGACGGACTCCTGGTTAGGAACTGCGCTCAAGTCCTTG CTTCTCATCCAGTCTCGAAAGAACTGTGCGAATGTTCTGATCACTACCACGCAGTTGGTTCCAGCCCTGG CCAAGGTTCTCCTGTATGGACTAGGAGAGATATTTCCTATTGAAAACATCTACAGTGCTACCAAAATCGG TAAGGAGAGCTGCTTTGAGAGAATTGTTTCGAGGTTTGGGAAAAAAGTCACATATGTAGTGATTGGAGAT GGACGAGATGAAGAAATTGCAGCCAAGCAGCACAACATGCCCTTCTGGAGGATCACAAACCACGGAGATC TGGTGTCCCTGCACCAGGCTTTAGAGCTTGACTTCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208198 representing NM_010166
Red=Cloning site Green=Tags(s) MEEEQDLPEQPVKKAKMQEPREQTLSQVNNPDASDEKPETSSLASNLSMSEEIMTCTDYIPRSSNDYTSQ MYSAKPYAHILSVPVSETTYPGQTQYQTLQQSQPYAVYPQATQTYGLPPFASSTNASLIPTSSAIANIPA AAVASISNQDYPTYTILGQNQYQACYPSSSFGVTGQTNSDAETTTLAATTYQTEKPSAMVPAPATQRLPS DSSASPPLSQTTPNKDADDQARKNMTVKNRGKRKADASSSQDSELERVFLWDLDETIIIFHSLLTGSYAQ KYGKDPTVVIGSGLTMEEMIFEVADTHLFFNDLEECDQVHVEDVASDDNGQDLSNYSFSTDGFSGSGGSG SHGSSVGVQGGVDWMRKLAFRYRKVREIYDKHKSNVGGLLSPQRKEALQRLRAEIEVLTDSWLGTALKSL LLIQSRKNCANVLITTTQLVPALAKVLLYGLGEIFPIENIYSATKIGKESCFERIVSRFGKKVTYVVIGD GRDEEIAAKQHNMPFWRITNHGDLVSLHQALELDFL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010166 |
ORF Size | 1530 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010166.2, NP_034296.2 |
RefSeq Size | 5204 bp |
RefSeq ORF | 1533 bp |
Locus ID | 14050 |
UniProt ID | P97480 |
Gene Summary | Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1 (By similarity). Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. The phosphatase activity has been shown in vitro. Coactivates SIX1. Seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity. May be involved in development of the eye. May play a role in mediating the induction and differentiation of cranial placodes.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202813 | Eya3 (untagged) - Mouse eyes absent 3 homolog (Drosophila) (Eya3), transcript variant 2, (10ug) |
CNY 3,928.00 |
|
MR208198 | Eya3 (Myc-DDK-tagged) - Mouse eyes absent 3 homolog (Drosophila) (Eya3), transcript variant 2 |
CNY 3,928.00 |
|
MR208198L3 | Lenti ORF clone of Eya3 (Myc-DDK-tagged) - Mouse eyes absent 3 homolog (Drosophila) (Eya3), transcript variant 2 |
CNY 6,370.00 |
|
MR208198L4 | Lenti ORF clone of Eya3 (mGFP-tagged) - Mouse eyes absent 3 homolog (Drosophila) (Eya3), transcript variant 2 |
CNY 6,370.00 |