Fgr (NM_010208) Mouse Tagged ORF Clone

CAT#: MG208281

  • TrueORF®

Fgr (tGFP-tagged) - Mouse Gardner-Rasheed feline sarcoma viral (Fgr) oncogene homolog (Fgr)

ORF Plasmid: DDK tGFP



  "NM_010208" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 4,940.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG208281 representing NM_010208
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCTGTGTGTTCTGCAAGAAGTTGGAGCCTGCATCCAAGGAGGATGTGGGCCTGGAAGGGGACTTCC
GGAGCCAAACGGCTGAAGAACGCTATTACCCTGACCCCACTCAAGGCCGGACTTCGTCCGTCTTTCCTCA
GCCCACCAGCCCTGCTTTCCTCAACACTGGCAACATGAGAAGCATCTCAGGGACCGGAGTGACCATATTC
GTCGCCCTGTACGACTATGAGGCCAGGACAGGGGATGACCTCACCTTCACCAAAGGCGAGAAGTTCCACA
TCCTGAACAATACGGAGTATGACTGGTGGGAGGCTCGCTCCCTGAGCTCCGGACACAGAGGCTATGTTCC
CAGCAACTATGTTGCTCCTGTGGATTCCATCCAGGCTGAAGAGTGGTACTTCGGAAAGATCAGTAGAAAG
GATGCAGAGAGGCAGCTTCTGTCCTCTGGTAACCCCCAGGGGGCCTTTCTCATTCGGGAAAGCGAGACCA
CCAAAGGGGCCTACTCCCTGTCCATCCGTGACTGGGACCAGAACAGAGGCGATCACATAAAGCATTATAA
GATCCGAAAGCTGGACACGGGCGGCTACTACATCACCACACGGGCCCAGTTTGACTCCATACAGGACCTA
GTGCGGCACTACATGGAAGTGAATGATGGTCTGTGCTACTTGCTTACGGCGCCTTGTACCACCACTAAGC
CCCAGACTCTAGGCCTGGCCAAGGATGCCTGGGAGATCGACCGGAACTCCATAGCACTGGAACGCAGGCT
GGGCACCGGCTGCTTTGGAGATGTGTGGCTGGGCACATGGAACTGCAGCACAAAGGTGGCAGTGAAGACG
CTGAAGCCGGGCACCATGTCCCCGAAGGCATTCCTGGAGGAGGCACAGATCATGAAGCTGCTGAGGCACG
ACAAGCTGGTGCAGCTGTATGCGGTGGTGTCGGAGGAACCCATTTATATTGTGACAGAGTTCATGTGCTA
TGGTAGCTTGCTGGATTTCCTAAAGGATCGAGAAGGTCAGAACTTGATGCTGCCCCATCTAGTGGACATG
GCTGCCCAGGTAGCCGAGGGCATGGCCTACATGGAACGCATGAACTATATCCACCGAGACTTGAGGGCAG
CCAACATCCTGGTGGGGGAATACCTAATATGCAAGATCGCTGACTTCGGGCTGGCACGCCTCATAGAGGA
CAATGAGTATAACCCCCAACAAGGAACCAAGTTCCCCATCAAGTGGACAGCCCCAGAGGCCGCCCTCTTT
GGCAGATTCACTGTCAAATCAGACGTGTGGTCCTTTGGGATTCTGCTCACTGAACTGATCACCAAGGGCA
GAGTTCCCTACCCAGGTATGAACAACCGGGAAGTGTTGGAACAGGTGGAGCATGGCTACCACATGCCGTG
CCCTCCAGGATGTCCTGCATCCCTGTATGAGGTCATGGAGCAGGCCTGGCGCCTGGATCCAGAGGAGAGG
CCCACCTTTGAGTACCTGCAGTCTTTCCTGGAAGACTATTTCACCTCCACAGAACCACAGTACCAGCCTG
GAGACCAGACA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG208281 representing NM_010208
Red=Cloning site Green=Tags(s)

MGCVFCKKLEPASKEDVGLEGDFRSQTAEERYYPDPTQGRTSSVFPQPTSPAFLNTGNMRSISGTGVTIF
VALYDYEARTGDDLTFTKGEKFHILNNTEYDWWEARSLSSGHRGYVPSNYVAPVDSIQAEEWYFGKISRK
DAERQLLSSGNPQGAFLIRESETTKGAYSLSIRDWDQNRGDHIKHYKIRKLDTGGYYITTRAQFDSIQDL
VRHYMEVNDGLCYLLTAPCTTTKPQTLGLAKDAWEIDRNSIALERRLGTGCFGDVWLGTWNCSTKVAVKT
LKPGTMSPKAFLEEAQIMKLLRHDKLVQLYAVVSEEPIYIVTEFMCYGSLLDFLKDREGQNLMLPHLVDM
AAQVAEGMAYMERMNYIHRDLRAANILVGEYLICKIADFGLARLIEDNEYNPQQGTKFPIKWTAPEAALF
GRFTVKSDVWSFGILLTELITKGRVPYPGMNNREVLEQVEHGYHMPCPPGCPASLYEVMEQAWRLDPEER
PTFEYLQSFLEDYFTSTEPQYQPGDQT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_010208
ORF Size 1551 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_010208.2, NP_034338.2
RefSeq Size 3254 bp
RefSeq ORF 1554 bp
Locus ID 14191
UniProt ID P14234
Gene Summary Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors devoid of kinase activity and contributes to the regulation of immune responses, including neutrophil, monocyte, macrophage and mast cell functions, cytoskeleton remodeling in response to extracellular stimuli, phagocytosis, cell adhesion and migration. Promotes mast cell degranulation, release of inflammatory cytokines and IgE-mediated anaphylaxis. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as MS4A2/FCER1B, FCER1G and FCGR2. Acts downstream of ITGB1 and ITGB2, and regulates actin cytoskeleton reorganization, cell spreading and adhesion. Depending on the context, activates or inhibits cellular responses. Functions as negative regulator of ITGB2 signaling, phagocytosis and SYK activity in monocytes (PubMed:11672534). Required for normal ITGB1 and ITGB2 signaling, normal cell spreading and adhesion in neutrophils and macrophages (PubMed:8666673 and PubMed:9687507). Functions as positive regulator of cell migration and regulates cytoskeleton reorganization via RAC1 activation (PubMed:15561106). Phosphorylates SYK (in vitro) and promotes SYK-dependent activation of AKT1 and MAP kinase signaling (PubMed:21746961). Phosphorylates PLD2 in antigen-stimulated mast cells, leading to PLD2 activation and the production of the signaling molecules lysophosphatidic acid and diacylglycerol. Promotes activation of PIK3R1. Phosphorylates FASLG, and thereby regulates its ubiquitination and subsequent internalization. Phosphorylates ABL1. Promotes phosphorylation of CBL, CTTN, PIK3R1, PTK2/FAK1, PTK2B/PYK2 and VAV2. Phosphorylates HCLS1 that has already been phosphorylated by SYK, but not unphosphorylated HCLS1.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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