Aldh1a2 (BC075704) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	RALDH-2, Aldh1a7, Raldh1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208298 representing BC075704 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTTCCAGCGAGATCGCCATGCCGGGCGAGGTGAAGGCCGACCCCGCCGCGCTCATGGCCTCGCTGC AGCTCCTGCCGTCGCCCACGCCCAACCTCGAGATCAAGTACACCAAGATCTTTATTAACAATGAATGGCA GAACTCAGAGAGTGGGAGAGTGTTCCCTGTCTGTAATCCAGCCACAGGAGAGCAAGTGTGTGAAGTTCAA GAAGCAGACAAGGTGGATATAGACAAGGCAGTGCAAGCAGCCCGCCTTGCCTTCTCTCTTGGTTCTGTGT GGAGAAGGATGGATGCGTCTGAAAGAGGACGTCTGTTGGACAAGCTTGCAGACTTGGTGGAACGGGACAG GGCAACTCTTGCAACTATGGAATCCCTAAATGGCGGTAAGCCATTCCTGCAAGCTTTTTACATCGATTTG CAGGGAGTCATCAAAACCCTGAGGTATTATGCAGGCTGGGCTGATAAAATTCACGGAATGACCATTCCTG TAGATGGAGACTATTTTACTTTCACAAGACACGAGCCCATTGGAGTGTGTGGACAGATCATCCCGTGGAA CTTCCCCCTGCTGATGTTCACCTGGAAAATTGCTCCCGCATTGTGCTGTGGTAACACCGTGGTCATCAAA CCCGCTGAGCAGACACCGCTCAGTGCACTGTATATGGGAGCCCTCATCAAGGAGGCTGGCTTTCCACCCG GAGTCGTCAATATCCTGCCAGGGTATGGGCCAACAGCAGGGGCAGCAATCGCTTCTCACATCGGCATAGA CAAGATTGCATTCACAGGCTCTACTGAGGTTGGAAAGCTTATTCAAGAAGCAGCTGGAAGAAGTAACCTG AAGAGAGTGACCCTGGAACTCGGAGGGAAGAGCCCTAATATTATTTTTGCAGATGCTGACTTGGACTACG CTGTGGAGCAGGCTCACCAGGGTGTGTTCTTCAACCAAGGCCAGTGCTGCACCGCAGGGTCTCGCATCTT TGTGGAAGAATCCATCTATGAGGAATTTGTGAAAAGAAGTGTGGAAAGGGCCAAGAGGCGCATTGTGGGG AGCCCCTTTGATCCCACAACTGAGCAGGGTCCTCAGATCGATAAGAAACAATACAACAAAGTCCTGGAGC TTATCCAGAGCGGTGTGGCTGAGGGCGCCAAGCTGGAGTGCGGAGGCAAAGGGCTGGGAAGGAAGGGCTT CTTCATTGAGCCCACCGTGTTCTCCAACGTCACTGATGACATGCGGATTGCCAAGGAGGAGATCTTTGGT CCTGTTCAAGAAATTTTGAGGTTTAAGACTATGGATGAAGTTATAGAAAGAGCCAATAACTCAGACTTTG GACTTGTAGCAGCTGTCTTCACTAATGACATCAACAAGGCTCTCATGGTATCCTCCGCAATGCAAGCTGG GACAGTTTGGATCAATTGTTACAATGCCTTAAATGCGCAGAGCCCCTTTGGGGGGTTCAAGATGTCTGGA AATGGGAGAGAAATGGGTGAGTTTGGCTTACGGGAGTATTCAGAAGTAAAGACCGTGACGGTGAAGATCC CCCAGAAGAACTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208298 representing BC075704 Red=Cloning site Green=Tags(s) MTSSEIAMPGEVKADPAALMASLQLLPSPTPNLEIKYTKIFINNEWQNSESGRVFPVCNPATGEQVCEVQ EADKVDIDKAVQAARLAFSLGSVWRRMDASERGRLLDKLADLVERDRATLATMESLNGGKPFLQAFYIDL QGVIKTLRYYAGWADKIHGMTIPVDGDYFTFTRHEPIGVCGQIIPWNFPLLMFTWKIAPALCCGNTVVIK PAEQTPLSALYMGALIKEAGFPPGVVNILPGYGPTAGAAIASHIGIDKIAFTGSTEVGKLIQEAAGRSNL KRVTLELGGKSPNIIFADADLDYAVEQAHQGVFFNQGQCCTAGSRIFVEESIYEEFVKRSVERAKRRIVG SPFDPTTEQGPQIDKKQYNKVLELIQSGVAEGAKLECGGKGLGRKGFFIEPTVFSNVTDDMRIAKEEIFG PVQEILRFKTMDEVIERANNSDFGLVAAVFTNDINKALMVSSAMQAGTVWINCYNALNAQSPFGGFKMSG NGREMGEFGLREYSEVKTVTVKIPQKNS TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC075704
ORF Size	1556 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC075704, AAH75704
RefSeq Size	2242 bp
RefSeq ORF	1556 bp
Locus ID	19378
Gene Summary	Converts retinaldehyde to retinoic acid (PubMed:8797830). Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal (By similarity). Lacks activity with benzaldehyde, acetaldehyde and octanal (PubMed:8797830). Displays complete lack of activity with citral (By similarity).[UniProtKB/Swiss-Prot Function]

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