Aldh6a1 (NM_134042) Mouse Tagged ORF Clone

CAT#: MG208569

  • TrueORF®

Aldh6a1 (tGFP-tagged) - Mouse aldehyde dehydrogenase family 6, subfamily A1 (Aldh6a1)

ORF Plasmid: DDK tGFP



  "NM_134042" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 5,592.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 1110038I05Rik; Mmsd; Mmsdh
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG208569 representing NM_134042
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGCAGTGGCGGCAGCGGCAGCAATGCGGTCCCGGATCCTGCAGGTTTCTTCTAAGGTAAATG
CCACATGGTATCCAGCATCCTCCTTCTCTTCCTCATCAGTGCCAACTGTAAAGCTCTTCATTGATGGAAA
ATTTGTTGAATCAAAAAGTGATAAATGGATTGACATCCACAACCCAGCCACCAATGAAGTTGTTGGTAGG
GTTCCTCAGTCCACCAAAGCTGAGATGGACGCAGCCGTTGAGTCCTGCAAACGTGCTTTCCCTGCGTGGG
CAGACACATCTATTTTAAGCCGGCAGCAGGTCCTGCTTCGTTATCAGCAGCTCATTAAAGAAAACCTGAA
AGAAATTGCCAGGTTAATCACACTGGAACAAGGGAAGACTCTTGCTGATGCTGAAGGAGATGTATTCCGA
TGCCTTCAGGTGGTTGAGCATGCCTGTAGTGTGACATCCCTCATGCTGGGAGAGACCATGCCATCTATCA
CCAAAGACATGGACCTTTATTCCTACCGTCTGCCTCTGGGGGTGTGTGCAGGCATTGCACCATTTAATTT
TCCTGCCATGATCCCCCTTTGGATGTTTCCCATGGCTATGGTTTGTGGAAATACTTTCCTAATGAAACCA
TCAGAGCGAGTACCTGGAGCAACTATGCTCCTTGCTAAGTTGCTTCAGGATTCTGGGGCCCCTGACGGAA
CACTGAACATTATCCATGGACAACATGATGCTGTAAACTTTATTTGCGATCATCCAGACATCAAGGCAAT
CAGCTTTGTGGGATCCAACCAGGCAGGAGAGTACATCTTCGAAAGAGGATCAAGAAATGGCAAGAGGGTT
CAAGCCAATATGGGAGCCAAAAACCATGGAGTAGTCATGCCAGACGCCAATAAGGAAAATACCCTCAACC
AGCTGGTCGGGGCAGCATTCGGAGCTGCTGGGCAGCGCTGCATGGCTCTTTCAACAGCCATCCTCGTAGG
GGAGGCTAAGAAGTGGCTGCCAGAACTGGTCGATCGGGCCAAAAATCTGCGAGTCAATGCAGGAGACCAG
CCTGGAGCTGATCTTGGCCCCCTGATCACCCCCCAGGCCAAAGAGAGAGTCTGCAATCTGATCGATAGCG
GAACAAAAGAGGGAGCTTCCATCCTTCTGGATGGGCGAAGAATTAAAGTCAAGGGCTATGAAAATGGTAA
CTTTGTTGGACCAACCATCATCTCAAATGTTAAGCCAAGTATGACTTGTTACAAAGAGGAGATATTTGGT
CCAGTTCTTGTGGTTTTGGAGACAGAAACACTGGATGAAGCAATCAAGATTGTAAATGACAACCCATATG
GAAATGGAACTGCCATCTTCACCACCAATGGAGCCACCGCCCGGAAATATGCTCACATGGTGGATGTGGG
ACAGGTGGGTGTGAATGTCCCCATTCCAGTGCCTTTGCCGATGTTTTCCTTCACCGGCTCTCGCTCTTCC
TTCAGGGGAGACACCAATTTCTATGGCAAACAGGGCATCCAGTTTTACACGCAGTTAAAGACTATCACGT
CTCAGTGGAAAGAAGAAGATGCTACGCTTTCCTCCCCTGCTGTAGTCATGCCTACCATGGGGCGT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG208569 representing NM_134042
Red=Cloning site Green=Tags(s)

MAAAVAAAAAMRSRILQVSSKVNATWYPASSFSSSSVPTVKLFIDGKFVESKSDKWIDIHNPATNEVVGR
VPQSTKAEMDAAVESCKRAFPAWADTSILSRQQVLLRYQQLIKENLKEIARLITLEQGKTLADAEGDVFR
CLQVVEHACSVTSLMLGETMPSITKDMDLYSYRLPLGVCAGIAPFNFPAMIPLWMFPMAMVCGNTFLMKP
SERVPGATMLLAKLLQDSGAPDGTLNIIHGQHDAVNFICDHPDIKAISFVGSNQAGEYIFERGSRNGKRV
QANMGAKNHGVVMPDANKENTLNQLVGAAFGAAGQRCMALSTAILVGEAKKWLPELVDRAKNLRVNAGDQ
PGADLGPLITPQAKERVCNLIDSGTKEGASILLDGRRIKVKGYENGNFVGPTIISNVKPSMTCYKEEIFG
PVLVVLETETLDEAIKIVNDNPYGNGTAIFTTNGATARKYAHMVDVGQVGVNVPIPVPLPMFSFTGSRSS
FRGDTNFYGKQGIQFYTQLKTITSQWKEEDATLSSPAVVMPTMGR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_134042
ORF Size 1605 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_134042.2, NP_598803.1
RefSeq Size 3346 bp
RefSeq ORF 1608 bp
Locus ID 104776
UniProt ID Q9EQ20
Gene Summary This gene encodes a member of the aldehyde dehydrogenase protein family. The encoded enzyme is a mitochondrial methylmalonate semialdehyde dehydrogenase that plays a role in the valine and pyrimidine catabolic pathways. This enzyme catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Mutations in the human gene result in Methylmalonate Semialdehyde Dehydrogenase Deficiency, characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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