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Aldh6a1 (NM_134042) Mouse Tagged ORF Clone
CAT＃: MR208569
- TrueORF®
Aldh6a1 (Myc-DDK-tagged) - Mouse aldehyde dehydrogenase family 6, subfamily A1 (Aldh6a1), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_134042" in other vectors (4)
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CNY 3,992.00
CNY 4,660.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110038I05Rik; Mmsd; Mmsdh |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208569 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCAGTGGCGGCAGCGGCAGCAATGCGGTCCCGGATCCTGCAGGTTTCTTCTAAGGTAAATG CCACATGGTATCCAGCATCCTCCTTCTCTTCCTCATCAGTGCCAACTGTAAAGCTCTTCATTGATGGAAA ATTTGTTGAATCAAAAAGTGATAAATGGATTGACATCCACAACCCAGCCACCAATGAAGTTGTTGGTAGG GTTCCTCAGTCCACCAAAGCTGAGATGGACGCAGCCGTTGAGTCCTGCAAACGTGCTTTCCCTGCGTGGG CAGACACATCTATTTTAAGCCGGCAGCAGGTCCTGCTTCGTTATCAGCAGCTCATTAAAGAAAACCTGAA AGAAATTGCCAGGTTAATCACACTGGAACAAGGGAAGACTCTTGCTGATGCTGAAGGAGATGTATTCCGA TGCCTTCAGGTGGTTGAGCATGCCTGTAGTGTGACATCCCTCATGCTGGGAGAGACCATGCCATCTATCA CCAAAGACATGGACCTTTATTCCTACCGTCTGCCTCTGGGGGTGTGTGCAGGCATTGCACCATTTAATTT TCCTGCCATGATCCCCCTTTGGATGTTTCCCATGGCTATGGTTTGTGGAAATACTTTCCTAATGAAACCA TCAGAGCGAGTACCTGGAGCAACTATGCTCCTTGCTAAGTTGCTTCAGGATTCTGGGGCCCCTGACGGAA CACTGAACATTATCCATGGACAACATGATGCTGTAAACTTTATTTGCGATCATCCAGACATCAAGGCAAT CAGCTTTGTGGGATCCAACCAGGCAGGAGAGTACATCTTCGAAAGAGGATCAAGAAATGGCAAGAGGGTT CAAGCCAATATGGGAGCCAAAAACCATGGAGTAGTCATGCCAGACGCCAATAAGGAAAATACCCTCAACC AGCTGGTCGGGGCAGCATTCGGAGCTGCTGGGCAGCGCTGCATGGCTCTTTCAACAGCCATCCTCGTAGG GGAGGCTAAGAAGTGGCTGCCAGAACTGGTCGATCGGGCCAAAAATCTGCGAGTCAATGCAGGAGACCAG CCTGGAGCTGATCTTGGCCCCCTGATCACCCCCCAGGCCAAAGAGAGAGTCTGCAATCTGATCGATAGCG GAACAAAAGAGGGAGCTTCCATCCTTCTGGATGGGCGAAGAATTAAAGTCAAGGGCTATGAAAATGGTAA CTTTGTTGGACCAACCATCATCTCAAATGTTAAGCCAAGTATGACTTGTTACAAAGAGGAGATATTTGGT CCAGTTCTTGTGGTTTTGGAGACAGAAACACTGGATGAAGCAATCAAGATTGTAAATGACAACCCATATG GAAATGGAACTGCCATCTTCACCACCAATGGAGCCACCGCCCGGAAATATGCTCACATGGTGGATGTGGG ACAGGTGGGTGTGAATGTCCCCATTCCAGTGCCTTTGCCGATGTTTTCCTTCACCGGCTCTCGCTCTTCC TTCAGGGGAGACACCAATTTCTATGGCAAACAGGGCATCCAGTTTTACACGCAGTTAAAGACTATCACGT CTCAGTGGAAAGAAGAAGATGCTACGCTTTCCTCCCCTGCTGTAGTCATGCCTACCATGGGGCGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208569 protein sequence
Red=Cloning site Green=Tags(s) MAAAVAAAAAMRSRILQVSSKVNATWYPASSFSSSSVPTVKLFIDGKFVESKSDKWIDIHNPATNEVVGR VPQSTKAEMDAAVESCKRAFPAWADTSILSRQQVLLRYQQLIKENLKEIARLITLEQGKTLADAEGDVFR CLQVVEHACSVTSLMLGETMPSITKDMDLYSYRLPLGVCAGIAPFNFPAMIPLWMFPMAMVCGNTFLMKP SERVPGATMLLAKLLQDSGAPDGTLNIIHGQHDAVNFICDHPDIKAISFVGSNQAGEYIFERGSRNGKRV QANMGAKNHGVVMPDANKENTLNQLVGAAFGAAGQRCMALSTAILVGEAKKWLPELVDRAKNLRVNAGDQ PGADLGPLITPQAKERVCNLIDSGTKEGASILLDGRRIKVKGYENGNFVGPTIISNVKPSMTCYKEEIFG PVLVVLETETLDEAIKIVNDNPYGNGTAIFTTNGATARKYAHMVDVGQVGVNVPIPVPLPMFSFTGSRSS FRGDTNFYGKQGIQFYTQLKTITSQWKEEDATLSSPAVVMPTMGR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_134042 |
| ORF Size | 1608 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_134042.1, NM_134042.2, NM_134042.3, NP_598803.1 |
| RefSeq Size | 3346 bp |
| RefSeq ORF | 1608 bp |
| Locus ID | 104776 |
| UniProt ID | Q9EQ20 |
| MW | 58 kDa |
| Gene Summary | This gene encodes a member of the aldehyde dehydrogenase protein family. The encoded enzyme is a mitochondrial methylmalonate semialdehyde dehydrogenase that plays a role in the valine and pyrimidine catabolic pathways. This enzyme catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Mutations in the human gene result in Methylmalonate Semialdehyde Dehydrogenase Deficiency, characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204013 | Aldh6a1 (untagged) - Mouse aldehyde dehydrogenase family 6, subfamily A1 (Aldh6a1), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 3,992.00 |
|
| MG208569 | Aldh6a1 (tGFP-tagged) - Mouse aldehyde dehydrogenase family 6, subfamily A1 (Aldh6a1) |
CNY 5,592.00 |
|
| MR208569L3 | Lenti ORF clone of Aldh6a1 (Myc-DDK-tagged) - Mouse aldehyde dehydrogenase family 6, subfamily A1 (Aldh6a1), nuclear gene encoding mitochondrial protein |
CNY 6,560.00 |
|
| MR208569L4 | Lenti ORF clone of Aldh6a1 (mGFP-tagged) - Mouse aldehyde dehydrogenase family 6, subfamily A1 (Aldh6a1), nuclear gene encoding mitochondrial protein |
CNY 6,560.00 |
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