Men1 (BC036287) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	menin
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208819 representing BC036287 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCTGAAGGCCGCCCAGAAGACGCTGTTCCCTCTGCGCTCTATCGACGACGTGGTGCGCCTGTTTG CTGCAGAGCTGGGCCGAGAGGAGCCTGACCTGGTGCTCCTGTCCTTGGTCCTGGGCTTCGTGGAGCATTT CCTGGCTGTCAACCGTGTCATCCCCACCAACGTGCCGGAGCTCACCTTCCAGCCCAGCCCCGCACCCGAC CCTCCTGGTGGCCTCACCTACTTCCCGGTGGCCGACCTATCCATCATTGCTGCCCTCTATGCCCGATTCA CCGCTCAGATCCGCGGCGCTGTGGACCTCTCCCTCTATCCTCGAGAGGGAGGTGTTTCTAGTCGCGAACT GGTAAAAAAGGTCTCGGATGTCATATGGAACAGCCTCAGCCGCTCCTACTTCAAGGACCGGGCCCACATC CAGTCCCTCTTCAGCTTCATCACAGGCACCAAACTGGACAGCTCGGGCGTGGCCTTTGCAGTGGTAGGGG CCTGCCAGGCTCTGGGTCTCAGAGATGTCCATCTGGCCCTGTCTGAAGATCATGCTTGGGTGGTGTTTGG GCCCAACGGGGAGCAGACAGCTGAGGTGACGTGGCACGGCAAAGGCAACGAGGACCGCAGAGGCCAGACA GTCAATGCCGGTGTGGCTGAGCGGAGCTGGCTGTACCTGAAGGGGTCGTACATGCGCTGCGACCGTAAGA TGGAGGTGGCGTTCATGGTGTGTGCCATCAACCCTTCCATCGATCTTCACACTGACTCTTTGGAACTGTT GCAACTACAGCAGAAGCTGCTCTGGCTGCTGTATGACCTCGGACATCTGGAAAGATACCCCATGGCGCTA GGGAACTTGGCAGACCTGGAGGAGCTGGAGCCTACCCCGGGCCGGCCAGACCCACTCACCCTTTATCACA AGGGAATTGCCTCAGCTAAGACCTACTACCAGGATGAACACATCTACCCCTACATGTACCTGGCTGGCTA CCACTGTCGCAACCGAAATGTGCGCGAAGCCCTGCAGGCCTGGGCCGACACTGCCACTGTTATCCAAGAC TACAACTACTGCCGGGAGGATGAGGAGATCTACAAGGAATTCTTTGAAGTGGCCAATGACGTCATCCCCA ACCTGCTGAAGGAGGCGGCCAGCCTGCTAGAAACAGGCGAAGAGCGGACTGGGGAGCAAGCCCAGGTGCG GCAGAAGGTGCACATAGTTAGCCGGGAAGCAGAGGCGGCCGAGGCTGAAGAACCATGGGGGGATGAAGCC CGAGAAGGCCGTCGGCGTGGTCCCCGAAGAGAGTCCAAGCCTGAGGAGCCACCACCACCCAAGAAGCCTG CATTGGACAAGGGCCCGGGCTCAGGACAAAGTGCAGGGTTGGGACCACCTAGGAAAACGTCAGGGACTGT CCCAGGTACTACCCGCGGTGGCCAGGAAGTCGGCAATGCTGCTCAGGCTCCAGCACCTGCAGCATCGCCA CCCCCAGAGGGCCCAGTGCTCACTTTCCAGAGTGAGAAGATGAAAGGCATGAAGGAGCTACTGGTGGCCA CCAAGATCAACTCGAGCGCCATCAAGCTGCAACTCACGGCACAGTCGCAAGTGCAGATGAAGAAACAGAA AGTGTCCACACCCAGCGACTACACACTCTCTTTCCTAAAGCGGCAGCGCAAGGGCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208819 representing BC036287 Red=Cloning site Green=Tags(s) MGLKAAQKTLFPLRSIDDVVRLFAAELGREEPDLVLLSLVLGFVEHFLAVNRVIPTNVPELTFQPSPAPD PPGGLTYFPVADLSIIAALYARFTAQIRGAVDLSLYPREGGVSSRELVKKVSDVIWNSLSRSYFKDRAHI QSLFSFITGTKLDSSGVAFAVVGACQALGLRDVHLALSEDHAWVVFGPNGEQTAEVTWHGKGNEDRRGQT VNAGVAERSWLYLKGSYMRCDRKMEVAFMVCAINPSIDLHTDSLELLQLQQKLLWLLYDLGHLERYPMAL GNLADLEELEPTPGRPDPLTLYHKGIASAKTYYQDEHIYPYMYLAGYHCRNRNVREALQAWADTATVIQD YNYCREDEEIYKEFFEVANDVIPNLLKEAASLLETGEERTGEQAQVRQKVHIVSREAEAAEAEEPWGDEA REGRRRGPRRESKPEEPPPPKKPALDKGPGSGQSAGLGPPRKTSGTVPGTTRGGQEVGNAAQAPAPAASP PPEGPVLTFQSEKMKGMKELLVATKINSSAIKLQLTAQSQVQMKKQKVSTPSDYTLSFLKRQRKGL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC036287
ORF Size	1668 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC036287, AAH36287
RefSeq Size	2782 bp
RefSeq ORF	1670 bp
Locus ID	17283
Gene Summary	Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression (By similarity). May be involved in normal hematopoiesis through the activation of HOXA9 expression. May be involved in DNA repair.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.