Pias2 (BC034711) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Dib, ARIP3, PIASxbeta, SIZ2, PIASxb, 4930520E11Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208919 representing BC034711 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTTCTAGTTTTAGGGTTTCTGAGTTACAAGTGTTACTGGGCTTTGCTGGACGGAATAAAAGTGGGC GCAAGCATGACCTCCTGATGAGGGCGTTGCATTTATTGAAGAGTGGCTGCAGCCCTGCGGTTCAGATTAA AATTCGAGAATTATATAGACGCCGATACCCACGGACACTTGAAGGACTTTGTGATCTTTCCACAATCAAA TCATCGGTTTTCAGCTTGGATGGTAGCTCATCACCCGTAGAACCTGACTTGCCTGTGGCTGGGATCCACT CGTTGCCTTCGACTTCAATTACACCTCATTCACCGTCATCTCCTGTTGGTTCTGTACTGCTGCAAGACAC TAAGCCCACCTTTGAGATGCAGCAGCCGTCTCCGCCCATTCCTCCTGTCCATCCTGATGTGCAGTTAAAA AACCTGCCTTTCTATGATGTCCTTGATGTTCTCATCAAGCCCACGAGTTTAGTTCAAAGCAGTATTCAGC GGTTTCAAGAGAAGTTTTTTATTTTTGCTTTGACACCTCAGCAAGTTAGAGAAATATGTATTTCAAGGGA CTTTTTGCCGGGTGGCAGGAGAGACTACACAGTCCAAGTCCAGCTGCGACTTTGCTTGGCAGAGACCAGT TGCCCTCAAGAAGATAACTATCCCAATAGTTTGTGTATAAAAGTCAATGGGAAACTCTTTCCTTTGCCTG GCTATGCGCCACCACCTAAAAATGGGATTGAACAGAAGCGCCCTGGACGCCCCCTGAACATTACGTCTTT AGTTAGGTTGTCTTCAGCTGTGCCAAATCAGATTTCTATTTCTTGGGCATCTGAAATTGGAAAGAATTAC TCCATGTCTGTGTATCTTGTACGACAGCTTACATCAGCCATGTTATTACAGAGATTAAAAATGAAAGGTA TTAGAAATCCTGATCATTCCAGAGCACTAATTAAAGAAAAACTTACTGCAGACCCTGATAGTGAAATTGC TACAACTAGTCTTCGAGTGTCCTTGATGTGCCCTTTAGGAAAAATGAGGCTGACAATCCCGTGCCGTGCA GTGACTTGTACACATCTGCAGTGCTTTGATGCTGCCCTCTATCTTCAGATGAATGAGAAAAAGCCCACCT GGATCTGTCCTGTTTGTGACAAAAAGGCTGCCTATGAGAGTCTGATACTAGATGGGCTTTTTATGGAAAT TCTCAATGACTGTTCTGATGTGGATGAGATCAAATTCCAGGAAGATGGTTCTTGGTGTCCCATGAGACCT AAAAAAGAAGCTATGAAAGTAACCAGCCAGCCCTGTACAAAAGTAGAAAGTTCAAGTGTCTTTAGTAAAC CTTGTTCAGTGACTGTAGCCAGTGATGCAAGCAAGAAGAAGATAGATGTTATTGATCTAACAATAGAGAG CTCTTCTGATGAAGAGGAAGACCCTCCCGCCAAAAGGAAATGCATCTTTATGTCAGAAACACAAAGCAGT CCAACCAAAGGGGTTCTCATGTATCAGCCATCTTCTGTAAGGGTGCCCAGTGTGACTTCAGTTGATCCTG CTGCTATTCCACCTTCATTAACAGACTACTCAGTACCATTCCACCACACGCCAGTGTCAAGCATGTCATC AGATTTGCCAGGAGAACAAAGAAGAAATGATATTAATAACGAAGTGCAGCTTGGAGCATCTTCTGATACT GTGCAACAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208919 representing BC034711 Red=Cloning site Green=Tags(s) MVSSFRVSELQVLLGFAGRNKSGRKHDLLMRALHLLKSGCSPAVQIKIRELYRRRYPRTLEGLCDLSTIK SSVFSLDGSSSPVEPDLPVAGIHSLPSTSITPHSPSSPVGSVLLQDTKPTFEMQQPSPPIPPVHPDVQLK NLPFYDVLDVLIKPTSLVQSSIQRFQEKFFIFALTPQQVREICISRDFLPGGRRDYTVQVQLRLCLAETS CPQEDNYPNSLCIKVNGKLFPLPGYAPPPKNGIEQKRPGRPLNITSLVRLSSAVPNQISISWASEIGKNY SMSVYLVRQLTSAMLLQRLKMKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLMCPLGKMRLTIPCRA VTCTHLQCFDAALYLQMNEKKPTWICPVCDKKAAYESLILDGLFMEILNDCSDVDEIKFQEDGSWCPMRP KKEAMKVTSQPCTKVESSSVFSKPCSVTVASDASKKKIDVIDLTIESSSDEEEDPPAKRKCIFMSETQSS PTKGVLMYQPSSVRVPSVTSVDPAAIPPSLTDYSVPFHHTPVSSMSSDLPGEQRRNDINNEVQLGASSDT VQQ TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC034711
ORF Size	1691 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC034711, AAH34711
RefSeq Size	2092 bp
RefSeq ORF	1691 bp
Locus ID	17344
Gene Summary	Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIASx-beta, but not isoform PIASx-alpha, promotes MDM2 sumoylation. Isoform PIASx-alpha promotes PARK7 sumoylation. Isoform PIASx-beta promotes NCOA2 sumoylation more efficiently than isoform PIASx-alpha (By similarity). Sumoylates PML at'Lys-65' and 'Lys-160' (By similarity).[UniProtKB/Swiss-Prot Function]

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