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Sh3bp1 (BC004598) Mouse Tagged ORF Clone
CAT＃: MG209303
- TrueORF®
Sh3bp1 (tGFP-tagged) - Mouse SH3-domain binding protein 1 (cDNA clone MGC:6037 IMAGE:3584958)
ORF Plasmid: DDK
"BC004598" in other vectors (4)
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CNY 6,064.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 3BP-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC004598 |
| ORF Size | 1802 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC004598, AAH04598 |
| RefSeq Size | 2224 bp |
| RefSeq ORF | 1802 bp |
| Locus ID | 20401 |
| Gene Summary | GTPase activating protein (GAP) which specifically converts GTP-bound Rho-type GTPases including RAC1 and CDC42 in their inactive GDP-bound form (PubMed:7621827). By specifically inactivating RAC1 at the leading edge of migrating cells, it regulates the spatiotemporal organization of cell protrusions which is important for proper cell migration. Also negatively regulates CDC42 in the process of actin remodeling and the formation of epithelial cell junctions. Through its GAP activity toward RAC1 and/or CDC42 plays a specific role in phagocytosis of large particles. Specifically recruited by a PI3 kinase/PI3K-dependent mechanism to sites of large particles engagement, inactivates RAC1 and/or CDC42 allowing the reorganization of the underlying actin cytoskeleton required for engulfment. It also plays a role in angiogenesis and the process of repulsive guidance as part of a semaphorin-plexin signaling pathway. Following the binding of PLXND1 to extracellular SEMA3E it dissociates from PLXND1 and inactivates RAC1, inducing the intracellular reorganization of the actin cytoskeleton and the collapse of cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200161 | Sh3bp1 (untagged) - Mouse SH3-domain binding protein 1 (cDNA clone MGC:6037 IMAGE:3584958), (10ug) |
CNY 4,472.00 |
|
| MR209303 | Sh3bp1 (Myc-DDK-tagged) - Mouse SH3-domain binding protein 1 (cDNA clone MGC:6037 IMAGE:3584958) |
CNY 4,464.00 |
|
| MR209303L3 | Lenti ORF clone of Sh3bp1 (Myc-DDK-tagged) - Mouse SH3-domain binding protein 1 (cDNA clone MGC:6037 IMAGE:3584958) |
CNY 5,320.00 |
|
| MR209303L4 | Lenti ORF clone of Sh3bp1 (mGFP-tagged) - Mouse SH3-domain binding protein 1 (cDNA clone MGC:6037 IMAGE:3584958) |
CNY 5,320.00 |
