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Grhl3 (NM_001013756) Mouse Tagged ORF Clone
CAT＃: MG209332
- TrueORF®
Grhl3 (tGFP-tagged) - Mouse grainyhead-like 3 (Drosophila) (Grhl3)
ORF Plasmid: DDK
"NM_001013756" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI561912; ct; Get1; Som; Tfcp2l4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209332 representing NM_001013756
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGAATGAACTTGATTTCAGGTCTGTGCGGTTGCTGAAGAATGACCCTGTGAGCTTCCAGAAGTTTC CCTACAGTAATGAGGACGAGGCCTGGAAGACATACCTGGAGAACCCTTTGACGGCTGCCACCAAAGCCAT GATGAGAGTCAACGGGGACGAGGAGAGTGTGGCTGCTCTGAGCTTCCTCTACGACTACTATATGGGTCCC AAGGAGAAGCGGATACTGTCCTCCAGCACTGGTGGCCGGAATGACCAAGGAAAGAAGTTCTACCACAGCA TGGACTATGAGCCGGATCTTGCCCCCCTCGAGAGCCCCACACACCTCATGAAATTTTTGACAGAGAACGT GTCTGGAAGTCCAGACTACACAGACCAGCTCAAGAAAAACAATCTGCTAGGCTTGGAGGGGGTTCTACCC ACCCCCGGCAAGACCAATACCGTCCCCCCAGGTCCGAGTAAACTGGAAGCCAGCTCCATGGACAGCTACC TCTTGCCCGCCAGTGACATATATGACAATGGCTCCCTCAACTCATTATTTGAGAGCATTCATGGGGTTCC ACCCACACAGCGCTGGCAGCCAGACAGCACCTTCAAAGATGACCCACAGGAGTCTCTGCTCTTCCCTGAT ATTCTGAAGACATCCCCGGACCCCCCATGCCCAGAGGATTATCCAGGCCTCAAGAGTGACTTTGAATACA CCCTGGGCTCCCCCAAAGCCATTCACATCAAAGCAGGGGAGTCACCCATGGCCTACCTCAACAAGGGTCA GTTCTACCCCGTCACCCTACGCACCCCAGCAGGAGGGAAAGGCCTCGCTCTGTCCTCCAGCAAAGTCAAG AGCGTGGTGATGGTCGTGTTCGATAATGACAAGGTCCCCGTGGAGCAGCTGCGTTTCTGGAGGCACTGGC ATTCCCGGCAGCCCACCGCCAAGCAGCGCGTCATCGACGTAGCTGACTGTAAGGAAAACTTCAACACGGT CCAGCACATTGAAGAGGTGGCCTATAACGCGCTGTCCTTTGTGTGGAATGTCAACGAGGAAGCCAAGGTG TTTATCGGTGTCAACTGTCTGAGCACAGACTTCTCCTCGCAGAAGGGAGTGAAGGGTGTCCCCCTGAACT TGCAAATTGACACCTATGACTGTGGAGCAGGCACTGAGCGCCTGGTACACCGTGCTGTCTGCCAGATCAA GATCTTCTGTGATAAGGGAGCTGAGAGGAAGATGCGCGATGATGAACGGAAGCAGTTTCGAAGGAAGGTC AAGTGCCCAGACTCCAGTAACAATGCAGGAATCAAGGGCTGCCTGCTGTCAGGCTTCAGGGGCAATGAGA CCACATACTTGCGGCCAGAAACTGACCTGGAGACCCAGCCTGTGTTGTTTATCCCCAATCTGCATTTTTC CAGCCTACAGCGCCCAGGAGGGGTTGTCCCCTCAGCAGGACACAGCAGCTCTGACAGGCTGCCTCTGAAG CGAACCTGCTCACCCTTTGCTGAGGAGTTTGAGCCTCTTCCTTCTAAACAAGCCAAGGAAGATGACCTTC AGAGAGTTCTGTTGTATGTGAGGAGGGAGACAGAGGAGGTGTTTGACGCGCTCATGTTGAAGACCCCGGA CCTGAAGGGCCTGAGGAATGCGATCTCTGAGAAGTACGGCCTCCCCGAGGAGAATATTTGCAAAGTCTAC AAGAAATGCAAGCGAGGCATCCTGGTTAACATGGACAACAACATCATCCAACACTACAGCAACCACGTGG CCTTCCTGCTGGACATGGGTGAGCTGGACGGCAAGATCCAGATCATCCTGAAGGAGCTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209332 representing NM_001013756
Red=Cloning site Green=Tags(s) MSNELDFRSVRLLKNDPVSFQKFPYSNEDEAWKTYLENPLTAATKAMMRVNGDEESVAALSFLYDYYMGP KEKRILSSSTGGRNDQGKKFYHSMDYEPDLAPLESPTHLMKFLTENVSGSPDYTDQLKKNNLLGLEGVLP TPGKTNTVPPGPSKLEASSMDSYLLPASDIYDNGSLNSLFESIHGVPPTQRWQPDSTFKDDPQESLLFPD ILKTSPDPPCPEDYPGLKSDFEYTLGSPKAIHIKAGESPMAYLNKGQFYPVTLRTPAGGKGLALSSSKVK SVVMVVFDNDKVPVEQLRFWRHWHSRQPTAKQRVIDVADCKENFNTVQHIEEVAYNALSFVWNVNEEAKV FIGVNCLSTDFSSQKGVKGVPLNLQIDTYDCGAGTERLVHRAVCQIKIFCDKGAERKMRDDERKQFRRKV KCPDSSNNAGIKGCLLSGFRGNETTYLRPETDLETQPVLFIPNLHFSSLQRPGGVVPSAGHSSSDRLPLK RTCSPFAEEFEPLPSKQAKEDDLQRVLLYVRRETEEVFDALMLKTPDLKGLRNAISEKYGLPEENICKVY KKCKRGILVNMDNNIIQHYSNHVAFLLDMGELDGKIQIILKEL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001013756 |
| ORF Size | 1809 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001013756.2 |
| RefSeq Size | 2798 bp |
| RefSeq ORF | 1812 bp |
| Locus ID | 230824 |
| UniProt ID | Q5FWH3 |
| Gene Summary | Transcription factor playing important roles in primary neurulation and in the differentiation of stratified epithelia of both ectodermal and endodermal origin. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' acting as an activator and repressor on distinct target genes. Essential for epidermal differentiation and barrier formation at the end of embryogenesis with TGM3 as critical direct target (PubMed:21081122, PubMed:20654612, PubMed:25347468). Exhibits functional redundancy with GRHL2 in epidermal morphogenetic events such as eyelid fusion and epidermal wound repair (PubMed:21081122). Despite being dispensable during normal epidermal homeostasis in the adulthood, is again required for barrier repair after immune-mediated epidermal damage, regulates distinct gene batteries in embryonic epidermal differentiation and adult epidermal barrier reformation after injury (PubMed:25347468). Plays unique and cooperative roles with GRHL2 in establishing distinct zones of primary neurulation. Essential for spinal closure, functions cooperatively with GRHL2 in closure 2 (forebrain/midbrain boundary) and posterior neuropore closure (PubMed:14608380, PubMed:20654612). Also required for proper development of the oral periderm (PubMed:24360809). No genetic interaction with GRHL1, no functional cooperativity due to diverse target gene selectivity (PubMed:21081122).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219575 | Grhl3 (untagged) - Mouse grainyhead-like 3 (Drosophila) (Grhl3), (10ug) |
CNY 7,700.00 |
|
| MR209332 | Grhl3 (Myc-DDK-tagged) - Mouse grainyhead-like 3 (Drosophila) (Grhl3) |
CNY 4,496.00 |
|
| MR209332L3 | Lenti ORF clone of Grhl3 (Myc-DDK-tagged) - Mouse grainyhead-like 3 (Drosophila) (Grhl3) |
CNY 6,460.00 |
|
| MR209332L4 | Lenti ORF clone of Grhl3 (mGFP-tagged) - Mouse grainyhead-like 3 (Drosophila) (Grhl3) |
CNY 6,460.00 |
