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Nxf1 (NM_016813) Mouse Tagged ORF Clone
CAT＃: MG209464
- TrueORF®
Nxf1 (tGFP-tagged) - Mouse nuclear RNA export factor 1 homolog (S. cerevisiae) (Nxf1)
ORF Plasmid: DDK
"NM_016813" in other vectors (4)
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CNY 5,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Mex67; Mvb1; Tap |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209464 representing NM_016813
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGAAGGGAAGTCATACAACGAGCATGATGACCGTGTTAGTTTCCCTCAAAGAAGAAAGAAAG GCCGGGGGCCTTTCCGATGGAAGTGTGGTGAGGGGAATCGTAGGTCTGGAAGAGGTGGTTCTGGTGTACA GTCTTCCCGATTTGAGGAAGATGATGGCGATGTAGCAATGAATGATCCCCAGGATGGCCCCCGAGTAAGA TACAATCCCTATACCAACCGGCCTAATCGTCGGGGAGATGGTTGGCATGATCGAGATCGAATTCATATTA CTGTACGGAGAGACAGAGCTCCTGCAGAGAGAGGAGGGGCTGGCACCAGTCAAGATGGGACCACCAAGAA CTGGTTCAAGATTACAATTCCTTATGGTAGAAAGTATGACAAAACGTGGCTTCTGAGCATGATTCAGAGC AAATGCAGTGTCCCCTTCAATCCCATCGAGTTTCACTATGAAAATACACGGGCCCATTTTTTTGTGGAGG ATGCCACTACTGCTTCTGCATTAAAGGGTGTCAACCATAAGATCCAGGATCGAGAAAACAGAAGGATATC TATCATCATCAATGCTTCTGCTCCACCCTACACTGTACAGAATGAACTGAAGCCCGAACAAATAGAGCAG CTCAAGCTGATCATGAGCAAACGATATGATGGCAACCAACAAGCACTTGACCTCAAGGGGCTCCGCTCAG ACCCAGATTTGGTGGCCCAGAACATCGATGTTGTTCTAAACCGCAGAAGCTGTATGGCAGCCACCCTGCG GATCATTGAAGAGAACATTCCTGAGCTATTGTCTTTGAACTTGAGCAGCAACAGGCTATACAAGCTGGAT GATATGTCTAGCATTGTGCAGAAGGCGCCAAACCTAAAGACCCTAAATCTCTCCGGAAACGAATTGAAGA CTGAGCGGGAATTAGACAAGATAAAAGGGCTGAAGCTGGAAGAGCTGTGGCTTGACAGAAACCCCATGTG TGACAACTTCGGAGACCAGTCCAGCTATATCAGCGCCATTCGCGAACGATTTCCCAAGTTATTACGCCTG GATGGCCATGAGCTACCCCCTCCAATTTCCTTTGATGTCGAAGCCCCTACAATGTTACCACCCTGCAAGG GAAGCTATTTTGGAACCGAGAACCTAAAGAGTCTGGTCCTGCGCTTCTTGCAGCAGTACTATGTAATTTA TGACTCAGGAGACCGCCAGGGTCTCCTGTATGCCTACCATGATGGCGCCTGTTGCTCGCTAAGCATTCCT TACAACCCTCAGAATCCTGTCCGAAAAAACTTAGCTGAATATGTCAAAGACAGCAGAAATGTGAAGAAGC TTAAAGAACCCACCCAGCGGTTCCGACTCTTAAAGCATACACGTCTTAATGTCGTTGCCTTCCTCAATGA GTTACCCAAAACTCAACATGACGTTAACGCCTTCGTGGTAGATATAAGTGCCCAAACAAGCACATTACTG TGTTTTTCTGTCAATGGAGTCTTCAAGGAAGTGGATGGGAAGTCTCGAGATTCTTTACGTGCTTTCACAA GAACGTTCATTGCTGTTCCTGCCAGCAATTCAGGGCTATGTATTGTAAATGATGAATTATTTGTGCGGAA TGCCAGCCCTGAAGAAATCCAAAGAGCCTTCGCCATGTCTGCACCCACACCTTCCTCCAGTCCAGTACCC ACGCTTTCCCCAGAACAGCAGGAAATGTTGCAAGCATTTTCTACCCAGTCTGGCATGAACCTTGAGTGGT CTCAGAAGTGCCTTCAAGACAATAACTGGGACTACACCAGATCTGCTCAGGCCTTCACTCTTCTCAAGGC CAAGGGAGAGATTCCAGAAGTAGCGTTTATGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209464 representing NM_016813
Red=Cloning site Green=Tags(s) MADEGKSYNEHDDRVSFPQRRKKGRGPFRWKCGEGNRRSGRGGSGVQSSRFEEDDGDVAMNDPQDGPRVR YNPYTNRPNRRGDGWHDRDRIHITVRRDRAPAERGGAGTSQDGTTKNWFKITIPYGRKYDKTWLLSMIQS KCSVPFNPIEFHYENTRAHFFVEDATTASALKGVNHKIQDRENRRISIIINASAPPYTVQNELKPEQIEQ LKLIMSKRYDGNQQALDLKGLRSDPDLVAQNIDVVLNRRSCMAATLRIIEENIPELLSLNLSSNRLYKLD DMSSIVQKAPNLKTLNLSGNELKTERELDKIKGLKLEELWLDRNPMCDNFGDQSSYISAIRERFPKLLRL DGHELPPPISFDVEAPTMLPPCKGSYFGTENLKSLVLRFLQQYYVIYDSGDRQGLLYAYHDGACCSLSIP YNPQNPVRKNLAEYVKDSRNVKKLKEPTQRFRLLKHTRLNVVAFLNELPKTQHDVNAFVVDISAQTSTLL CFSVNGVFKEVDGKSRDSLRAFTRTFIAVPASNSGLCIVNDELFVRNASPEEIQRAFAMSAPTPSSSPVP TLSPEQQEMLQAFSTQSGMNLEWSQKCLQDNNWDYTRSAQAFTLLKAKGEIPEVAFMK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016813 |
| ORF Size | 1854 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016813.2, NP_058093.2 |
| RefSeq Size | 2312 bp |
| RefSeq ORF | 1857 bp |
| Locus ID | 53319 |
| UniProt ID | Q99JX7 |
| Gene Summary | Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export. Also involved in nuclear export of m6A-containing mRNAs: interaction between SRSF3 and YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200759 | Nxf1 (untagged) - Mouse nuclear RNA export factor 1 homolog (S. cerevisiae) (Nxf1), (10ug) |
CNY 4,608.00 |
|
| MR209464 | Nxf1 (Myc-DDK-tagged) - Mouse nuclear RNA export factor 1 homolog (S. cerevisiae) (Nxf1) |
CNY 4,608.00 |
|
| MR209464L3 | Lenti ORF clone of Nxf1 (Myc-DDK-tagged) - Mouse nuclear RNA export factor 1 homolog (S. cerevisiae) (Nxf1) |
CNY 7,320.00 |
|
| MR209464L4 | Lenti ORF clone of Nxf1 (mGFP-tagged) - Mouse nuclear RNA export factor 1 homolog (S. cerevisiae) (Nxf1) |
CNY 7,320.00 |
