Grhl1 (BC013080) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	LBP-32, MGR
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209470 representing BC013080 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACACAGGAGTACGACAACAAAAGGCCCGTGCTGGTACTTCAGAATGAAGCCCTCTACCCACAGCGGC GCTCCTATACCAGTGAGGATGAAGCCTGGAAGTCGTTCCTGGAAAACCCTCTCACTGCGGCAACCAAAGC GATGATGAGCATCAACGGAGACGAAGACAGCGCGGCTGCGCTGGGCCTGCTCTATGACTACTACAAGGTC CCCAGAGAGCGCCGGTCATCAGCCGTAAAGCCCGAGGGAGAGCACCCAGAGCCAGAGCACAGCAAAAGAA ACAGCATACCAAATGTGACGGAGCAGCCCCTCATTTCTGCTGGAGAAAACAGGGTACAAGTGCTGAAAAA CGTGCCCTTCAACATCGTCCTCCCCCATAGCAACCAGCTGGGCATTGATAAGAGAGGCCATCTGACAGCT CCCGATACAACAGTCACTGTCTCCATAGCGACCATGCCTACCCACTCCATCAAGACAGAAATCCAGCCGC ACGGCTTTGCTGTGGGAATCCCTCCAGCCGTGTACCACTCTGAGCCCACCGAACGCGTGGTGGTTTTTGA CCGGAGCCTCAGCACTGATCAGTTCAGCTCTGGCACTCAGCCCCCCAATGCTCAGCGGAGGACTCCAGAC TCCACCTTCTCCGAGACCTTCAAGGAGGGCGTTCAGGAGGTTTTCTTCCCCTCGGAACTCAGCCTTCGGA TGCCGGGCATGAATTCAGAGGACTATGTCTTTGACAATGTTTCTGGGAACAACTTTGAGTATACCCTGGA AGCCTCCAAGTCACTGCGGCAGAAGCAAGGGGACAGCACTATGACATACCTGAATAAAGGCCAGTTCTAT CCTGTCACCTTAAAGGAAGGAAGCAGCAATGAAGGGATTCACCACCCTATCAGCAAAGTTCGAAGTGTGA TCATGGTGGTTTTTGCTGAAGACAAAAGCAGAGAAGACCAGCTGAGACACTGGAAGTACTGGCACTCCCG TCAGCACACGGCCAAACAGAGGTGCATTGACATTGCTGACTACAAAGAAAGTTTCAACACTATCAGCAAC ATTGAGGAGATAGCTTATAACGCCATTTCCTTCACGTGGGACATCAATGATGAGGCAAAGGTCTTCATCT CTGTGAACTGCTTGAGCACAGATTTCTCTTCTCAGAAGGGTGTGAAGGGCTTGCCACTCAACATTCAAAT CGACACATACAGCTATAACAACCGCAGCAACAAGCCGGTTCACCGGGCCTACTGCCAGATAAAGGTCTTC TGCGACAAGGGAGCTGAAAGGAAAATTCGGGATGAAGAACGAAAACAGAGCAAGAGAAAAGTGTCTGACG TTAAAGTGCAGCTGCTTCCCTCACACAAACGGACAGACATCACAGTGTTCAAGCCCTTCCTGGACCTCGA CACTCAGCCTGTCCTCTTCATTCCGGACGTGCATTTTACCAACCTGCAGCGGGGCAGTCATGTTCTTTCC CTCCCCTCTGAAGAACTGGAAGGTGAAGGCTCTGTCTTGAAAAGAGGGCCATTCGGAACCGAAGATGACT TTGGAGTTCCTCCTCCTGCTAAGCTGACTCGGACAGAAGAACCCAAGAGAGTGCTGCTCTATGTCCGAAA GGAATCAGAAGAAGTCTTCGACGCCCTGATGCTCAAGACGCCGTCTTTGAAGGGCCTGATGGAGGCAATT TCAGACAAGTATGATGTCCCCCATGACAAGATTGGGAAAATATTTAAGAAGTGCAAAAAAGGGATCCTCG TGAACATGGACGACAACATTGTGAAGCACTACTCCAATGAGGACACCTTCCAGCTGCAGATAGAGGAAGC CGGCGGCTCGTACAAGCTCACCCTGACAGAGATT AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209470 representing BC013080 Red=Cloning site Green=Tags(s) MTQEYDNKRPVLVLQNEALYPQRRSYTSEDEAWKSFLENPLTAATKAMMSINGDEDSAAALGLLYDYYKV PRERRSSAVKPEGEHPEPEHSKRNSIPNVTEQPLISAGENRVQVLKNVPFNIVLPHSNQLGIDKRGHLTA PDTTVTVSIATMPTHSIKTEIQPHGFAVGIPPAVYHSEPTERVVVFDRSLSTDQFSSGTQPPNAQRRTPD STFSETFKEGVQEVFFPSELSLRMPGMNSEDYVFDNVSGNNFEYTLEASKSLRQKQGDSTMTYLNKGQFY PVTLKEGSSNEGIHHPISKVRSVIMVVFAEDKSREDQLRHWKYWHSRQHTAKQRCIDIADYKESFNTISN IEEIAYNAISFTWDINDEAKVFISVNCLSTDFSSQKGVKGLPLNIQIDTYSYNNRSNKPVHRAYCQIKVF CDKGAERKIRDEERKQSKRKVSDVKVQLLPSHKRTDITVFKPFLDLDTQPVLFIPDVHFTNLQRGSHVLS LPSEELEGEGSVLKRGPFGTEDDFGVPPPAKLTRTEEPKRVLLYVRKESEEVFDALMLKTPSLKGLMEAI SDKYDVPHDKIGKIFKKCKKGILVNMDDNIVKHYSNEDTFQLQIEEAGGSYKLTLTEI SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	BC013080
ORF Size	1856 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC013080, AAH13080
RefSeq Size	3414 bp
RefSeq ORF	1856 bp
Locus ID	195733
Gene Summary	Transcription factor involved in epithelial development. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' (PubMed:18288204, PubMed:21081122). Important regulator of DSG1 in the context of hair anchorage and epidermal differentiation, participates in the maintenance of the skin barrier (PubMed:18288204, PubMed:24586629). There is no genetic interaction with GRHL3 no genetic interaction with GRHL3, no functional cooperativity due to diverse target gene selectivity during epithelia development (PubMed:21081122).[UniProtKB/Swiss-Prot Function]

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