Eif2ak1 (NM_013557) Mouse Tagged ORF Clone
CAT#: MG209482
- TrueORF®
Eif2ak1 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 1 (Eif2ak1)
ORF Plasmid: DDK
"NM_013557" in other vectors (6)
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CNY 5,990.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | HCR; Hri |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG209482 representing NM_013557
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGGGGGCAGCTCCGTGGACGGCGAGCGCGACACGGACGACGACGCGGCGGGGGCGGTGGCCGCGC CTCCTGCCATCGACTTCCCCGCAGAGGTGTCGGACCCCAAGTATGATGAGTCCGATGTCCCCGCAGAGCT CCAAGTGTTAAAAGAGCCCCTACAGCAGCCCACGTTCCCTTTCCTGGTGGCAAACCAGCTGCTGCTCGTC TCCTTACTGGAACACTTGAGCCATGTGCACGAGCCAAACCCGCTCCACTCCAAACAGGTGTTTAAATTAC TGTGCCAGACTTTTATCAAGATGGGGCTGCTCTCTTCCTTTACCTGCAGTGATGAGTTTAGCTCTCTGAG ACTCCACCACAACAGAGCCATCACTCATTTAATGAGGTCTGCCAAAGAGAGAGTCCGTCAGGATCCTTGT CAAGATAATTCTTACATGCAGAAAATCAGATCCAGAGAGATAGCCTTCGAAGCACAAACGTCACGCTACT TAAATGAATTTGAAGAGCTTGCCATCTTAGGGAAAGGAGGATATGGAAGAGTTTACAAGGTCCGGAACAA ATTAGATGGTCAGCATTATGCAATTAAGAAAATCCTGATTAAGAGCGCAACTAAAACAGATTGTATGAAG GTGCTACGGGAAGTGAAGGTTCTGGCAGGTCTCCAGCATCCCAATATTGTTGGCTACCACACTGCGTGGA TAGAACATGTTCATGTGGTTCAGCCACAAGACAGAGTTCCGATTCAACTGCCCTCTCTCGAAGTGCTGTC GGAGCAGGAAGGGGACAGAGACCAAGGTGGTGTTAAAGATAATGAAAGCAGTTCGTCCATTGTCTTTGCT GAACTCACCCCAGAAAAAGAAAAACCTTTTGGGGAGTCTGAGGTTAAAAATGAGAATAACAACCTGGTGA GCTACACGGCCAACTTAGTGGTCAGGAACAGCAGTGAAAGTGAATCGTCCATTGAGCTCCAAGAAGACGG CTTGACTGATTTGTCCGTCAGACCAGTTGTCAGGCATCAGCTGCCGCTGGGGCATAGCTCGGAATTGGAA GGGAATTTTACATCCACGGATGAGTCTTCTGAAGGCAACTTGAACCTGCTGGGGCAGACGGAGGTTCGGT ACCACCTGATGTTGCACATCCAGATGCAGCTGTGTGAGCTCTCCCTGTGGGACTGGATAACTGAGAGGAA CAAGCGGAGCCGGGAGTATGTGGACGAAGCTGCTTGTCCCTATGTTATGGCCAGTGTTGCAACAAAAATT TTTCAAGAATTGGTGGAAGGTGTCTTTTACATACATAACATGGGCATTGTCCACAGAGATCTGAAGCCTA GAAATATTTTTCTTCATGGCCCTGATCAGCAAGTAAAAATAGGAGACTTTGGTCTGGCCTGTGCAGACAT CATCCAGAATGCAGACTGGACCAACAGAAATGGGAAAGGAACACGGACACACACATCCAGAGTGGGGACT TGTCTCTACGCGTCACCGGAACAGCTGGAGGGATCCCAGTACGATGCCAAGTCAGATATGTATAGCTTGG GTGTGATCCTGCTCGAGCTCTTTCAGCCATTCGGGACAGAAATGGAGAGGGCAACAGTCTTAACAGGCGT AAGGACTGGTCGGATACCCGAATCCCTCAGTAAAAGGTGTCCGGTGCAAGCCAAGTATATCCAGCTCCTA ACCGGGAGGAATGCGTCACAGAGACCATCTGCCCTTCAGCTGCTGCAGAGTGAGCTTTTTCAAACAACTG GAAATGTTAATCTCACATTGCAGATGAAGATAATAGAACAAGAGAAGGAAATTGAAGAACTAAAGAAGCA ACTAAGCCTCCTTTCTCAGGACAGAGGGCTGAAGAGA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209482 representing NM_013557
Red=Cloning site Green=Tags(s) MLGGSSVDGERDTDDDAAGAVAAPPAIDFPAEVSDPKYDESDVPAELQVLKEPLQQPTFPFLVANQLLLV SLLEHLSHVHEPNPLHSKQVFKLLCQTFIKMGLLSSFTCSDEFSSLRLHHNRAITHLMRSAKERVRQDPC QDNSYMQKIRSREIAFEAQTSRYLNEFEELAILGKGGYGRVYKVRNKLDGQHYAIKKILIKSATKTDCMK VLREVKVLAGLQHPNIVGYHTAWIEHVHVVQPQDRVPIQLPSLEVLSEQEGDRDQGGVKDNESSSSIVFA ELTPEKEKPFGESEVKNENNNLVSYTANLVVRNSSESESSIELQEDGLTDLSVRPVVRHQLPLGHSSELE GNFTSTDESSEGNLNLLGQTEVRYHLMLHIQMQLCELSLWDWITERNKRSREYVDEAACPYVMASVATKI FQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACADIIQNADWTNRNGKGTRTHTSRVGT CLYASPEQLEGSQYDAKSDMYSLGVILLELFQPFGTEMERATVLTGVRTGRIPESLSKRCPVQAKYIQLL TGRNASQRPSALQLLQSELFQTTGNVNLTLQMKIIEQEKEIEELKKQLSLLSQDRGLKR SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_013557 |
ORF Size | 1857 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013557.2, NP_038585.2 |
RefSeq Size | 2805 bp |
RefSeq ORF | 1860 bp |
Locus ID | 15467 |
UniProt ID | Q9Z2R9 |
Gene Summary | Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202874 | Eif2ak1 (untagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 1 (Eif2ak1), (10ug) |
CNY 4,616.00 |
|
MR209482 | Eif2ak1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 1 (Eif2ak1) |
CNY 4,616.00 |
|
MR209482L1 | Lenti ORF clone of Eif2ak1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 1 (Eif2ak1) |
CNY 7,016.00 |
|
MR209482L2 | Lenti ORF clone of Eif2ak1 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 1 (Eif2ak1) |
CNY 7,320.00 |
|
MR209482L3 | Lenti ORF clone of Eif2ak1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 1 (Eif2ak1) |
CNY 7,320.00 |
|
MR209482L4 | Lenti ORF clone of Eif2ak1 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 1 (Eif2ak1) |
CNY 7,320.00 |
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