Tdrd3 (BC005670) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	4732418C03Rik; 6720468N18
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209760 representing BC005670 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCGCGTGCAGATGACGGATGGGCATACAAGTTGTACGGCAGTAGAATTCAGTTATATTTCAAAAA TAAGCCTGAACACACCACCTGGCACAAAAGTGAAGCTCTCTGGCACTGTGGACATAAAGAACGGATTCCT GCTCTTAAGTGACTCTAACACCACAGTTCTTGGTGGTGAAGTGGAACACCTAATTGACAAGTGGGCTTTA CAGAGAAGCTTATTAAAGCATAACAGAAGCAATATTGGAGCTGAAGGTGGCCCACCTCCTTTTCTACCTT TTGGGCAGAAGTGTGCATCTAATGTCCAAGTGGATAGCAGAGAGCTTGATCGAAGAAAGACATTACAAGT TTCCCTGCCTGCCAAGCCTGCAAATGATAATGATGAATTTGAAAAGCAAAGAACTGCTGCCATTGCAGAG GTTGCAAAGAGCAAAGAGACCAAGACATTTGGAGGAGGTGGAGGTGGTGCCAGAAGTAATCTCAATATTG GTGCTGCTGGTCACCGAAATAGGGAGGTTTTACAGAAGGAAAAGGCTTCCAAGTCAGAGAGCAAGAACGA AGGTGTCTATAGAGAATTGGTTGATGAGAAAGCCCTGAAACACATAACAGAAATGGGCTTCAGTAAGGAG GCTTCGAGGCAAGCGCTGATGGATAATGCCAACAACTTAGAAGCAGCACTGAATGTCCTTCTGAACAGCA GTAAACAGAAGCCTGCTGTGGGCCCTCCTGCTAGAGGAAGAGGGAAAGGCAGGGGTCGAGGACGATCAGA AGACGAGGAAGACCTGGGAACCGCAAGACCATCAGCACCAAGCACGTTATTTGATTTCTTGGAGTCTAAA ATGGGAACTCTGAATGTGGAAGAACCTAAGTCACAGCCACAGCACCTTCATCAAGGACAGCACAGAGGAT GGAATGCCGAGCAAAATGGGATGAAGGACGGCAGTCAGTCAAGACATCTTCCTCGAAACGATACCAGGCA GCCAAGAAATGAGAGGCCACCTCGCTTTCAAAAAGACACCCCAACTTCCAAGTCAACTGTAGAAAACAGC GTATTATCTAGAAATAGAGGCTCCGAAAGGCCGAGTAGTTCCTCAGGTTCTGATGTGTGGGCAGAAGAGA GGATCAGGTGTGACAGGCCATATTCTAGGTATGACAGAACTAAAGACGCTTCACATCCTCTAGGCTTTCA GCACAACGATGGTGCTTTTAAAAAGAGAGAGAACTCTATGCAAAACAGACCAGGAAGAGGCCCTTTGTAT GCAGAGGCCAAAGAAAACCCACTTCCTCCAGAATTCGTAGATTACAATAATCAAAGACGTGGGAGAAGAG AAAACCAAACAGGTCATCCTGATCACTGTTATGAGAGGAAACCACGGACAATGAATAGTGAAGCTTTTAG TGGTCTAAAAATTGAGAAACATTTTAGTGTAAACACTGATTACCCGAGTCCTGTGCAGAGCAATAGTCTT GGTGTTCCAAATGGAGAGACAGCTCCGCCTCTGAAAGGAAGGCGCGTAGGACCGATTAAGTCAGCAGGAC CTGTCACAGCTGTGCCCTACGATGATAAAATATTTTATAATAGTGGGCCCAAAAGAAGGTCTGGGCCAAT TAAGCCTGAAAAGGTGATAGAATCATCTATTCCTGTGGAGTATGCAAAAGTGTGGAAACCTGGCGATGAA TGTTTTGCACTTTATTGGGAAGACAACAAGTTTTACCGAGCAGAAGTTGAAGCCTTGCATTCTTCGGGTA TGACAGCGGTCGTTAAGTTCACGGACTACGGCAACTATGAAGAGGTGCTACTGAGCAATATCAAGCCTGT TCAGACAGAGGCATGGGAAGAAGAAGGCACCTATGATCACACCATTGAGTTTCGCAGAGGAGGTGATGGA CAGCCACGAAGGTCTACTCGGCCAACGCAGCAGTTTTACCAACCTCCTCGAGCTCGGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209760 representing BC005670 Red=Cloning site Green=Tags(s) MLRVQMTDGHTSCTAVEFSYISKISLNTPPGTKVKLSGTVDIKNGFLLLSDSNTTVLGGEVEHLIDKWAL QRSLLKHNRSNIGAEGGPPPFLPFGQKCASNVQVDSRELDRRKTLQVSLPAKPANDNDEFEKQRTAAIAE VAKSKETKTFGGGGGGARSNLNIGAAGHRNREVLQKEKASKSESKNEGVYRELVDEKALKHITEMGFSKE ASRQALMDNANNLEAALNVLLNSSKQKPAVGPPARGRGKGRGRGRSEDEEDLGTARPSAPSTLFDFLESK MGTLNVEEPKSQPQHLHQGQHRGWNAEQNGMKDGSQSRHLPRNDTRQPRNERPPRFQKDTPTSKSTVENS VLSRNRGSERPSSSSGSDVWAEERIRCDRPYSRYDRTKDASHPLGFQHNDGAFKKRENSMQNRPGRGPLY AEAKENPLPPEFVDYNNQRRGRRENQTGHPDHCYERKPRTMNSEAFSGLKIEKHFSVNTDYPSPVQSNSL GVPNGETAPPLKGRRVGPIKSAGPVTAVPYDDKIFYNSGPKRRSGPIKPEKVIESSIPVEYAKVWKPGDE CFALYWEDNKFYRAEVEALHSSGMTAVVKFTDYGNYEEVLLSNIKPVQTEAWEEEGTYDHTIEFRRGGDG QPRRSTRPTQQFYQPPRARN TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC005670
ORF Size	1952 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC005670, AAH05670
RefSeq Size	2625 bp
RefSeq ORF	1952 bp
Locus ID	219249
Gene Summary	Scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. In nucleus, acts as a coactivator: recognizes and binds asymmetric dimethylation on the core histone tails associated with transcriptional activation (H3R17me2a and H4R3me2a) and recruits proteins at these arginine-methylated loci. In cytoplasm, may play a role in the assembly and/or disassembly of mRNA stress granules and in the regulation of translation of target mRNAs by binding Arg/Gly-rich motifs (GAR) in dimethylarginine-containing proteins (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.