Atp13a2 (BC023746) Mouse Tagged ORF Clone

CAT#: MG209769

  • TrueORF®

Atp13a2 (tGFP-tagged) - Mouse ATPase type 13A2 (cDNA clone MGC:38540 IMAGE:5353766)

ORF Plasmid: DDK tGFP



  "BC023746" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 7,130.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 1110012E06Rik; AA589443
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG209769 representing BC023746
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAGCACGCCCCCCAGCTATGGGACCCTGACGACAGGGACGTCAATCGATCCCCTCAGCTCCTCAG
CTTCATCCGTGAGGCTCAGCGGCTACTGTGGCAGTCCATGGAGGGCCATCGGCTATCACGCCGCGGTCTG
GATGCTGGCCGGGATCCCTTGGCTGCTATTCCGCTGGAAGCCCTTGTGGGGCGTGCGCCTGCGCCTGAAG
CCATGCAGCCTGGCCCACGCCGAAACACTCGTTATAGAAATAAAAGACAAAGAGGGTAGCTCCAGACAGC
TCTTCACGGTTCAGGTGCAGACTGAGGCTCTTGTCCAGGGCAGCCTGGAGCTGCCCCCACAGGCCCAGGC
AGAGGATGGCCGGAGCCAGGCAGCCGTGGGAGTGACACCAGAGGGCACATGGCAGGACACCTCCGAACTC
CACAGGCAGGAAGAGGCAAAGCAGGTACTGCGCTATTATGTCCTCCAAGGTCAACGCTACGTCTGGATGG
AAACCCAGCAAGCCTTCTGCCAAGTCAGCCTGCTGGACCACAGTCGTACCTGTGATGACGTCCACTGCTC
CAGCTCCGGCCTCAGCCTCCAAGACCAAGCTACGAGGAAGACCATTTACGGCCCCAATGTGATCAGTATT
CCTGTCAAGTCCTACCTGCAGCTGCTGGCGGATGAGGCCCTGAATCCCTACTATGGGTTCCAAGCCTTCA
GCATCGCACTGTGGCTGGCCGACCACTACTACTGGTATGCTCTCTGCATCTTCCTCATCTCAGCCATCTC
CATCTGCCTGGCTCTGTACAAGACCAGAAAGCAAAGCTTGACCCTGAGGGACATGGTCAAGCTGTCCGTG
CGGGTGCAGGTGTGCCGACCCGGAGGAGAGGAAGAGTGGGTGGACTCCAGTGAGCTGGTGCCCGGAGACT
GCCTGGTGCTGCCCCAGGAGGGCGGGGTGATGCCATGCGACGCGGCTCTGGTGGCCGGGGAGTGTGTGGT
CAACGAGAGCTCCCTGACAGGGGAGAGCACCCCTGTGCTGAAGACGGCCCTGCCAGAGGGGCCCAAGCCC
TACTGCCCAGAGACCCACCGGCGGCACACCCTCTTCTGTGGCACCCTCATTCTGCAGGCCCGAGCCTACG
TGGGACCCCGTGTGCTAGCAGTGGTGACTCGGACAGGGTTCTGCACAGCCAAAGGGGGCCTGGTGAGCTC
CATCCTACACCCAAGGCCCATCAGCTTCAAGTTCTACAAACACAGCATGAAGTTTGTGGCTGCGCTCTCC
GTCCTGGCTCTGCTCGGCACCGTCTACAGCATCGTCATCCTCTACCGAAACCGGGTACCTGTGAGGGAGA
TTGTGATCCGAGCTCTGGACCTGGTGACGGTGGTGGTGCCGCCCGCCCTGCCTGCCGCCATGACGGTGTG
CACACTCTATGCCCAGAGTCGGCTGCGCGCACAGGGCATCTTCTGCATCCACCCACTGCGCATCAACTTG
GGGGGCAAGCTGCGGCTCGTGTGCTTTGACAAGACAGGCACCCTCACAGAGGACGGCTTGGACGTAATGG
GCGTAGTGCCCCTAAAGGGGCAGGTGTTGCTGCCACTGGTCCCAGAGCCCCGCCACCTGCCCCTGGGGCC
CCTCCTGCGAGCACTGGCCACCTGTCATGCCCTCAGCCAGCTACATGACACCCCGGTGGGCGACCCCATG
GATCTGAAGATGGTGGAGTCTACAGGCTGGGTCTTAGAGGAGGGTCCAGCTGCAGGCTCAGCACCTGGGA
GCCAGGTCTTGGTGGTAATGAGACCCCCACCCGGGGGCCCTCGGCAGCAGGTAAGCTGCAGAGTGACTGG
GCCTTGGGGCCTTGGAGTGGGCCTCAGCCAGCCCCTCAGCTCTCCTCCCACCCACAGGAAGAGCCACCAG
TGCCAGTCAGCGTCCTCTGCCGCTTCCCCTTCTCCTCTGCCCTGCAGCGGATGGATGTGG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG209769 representing BC023746
Red=Cloning site Green=Tags(s)

MGSTPPSYGTLTTGTSIDPLSSSASSVRLSGYCGSPWRAIGYHAAVWMLAGIPWLLFRWKPLWGVRLRLK
PCSLAHAETLVIEIKDKEGSSRQLFTVQVQTEALVQGSLELPPQAQAEDGRSQAAVGVTPEGTWQDTSEL
HRQEEAKQVLRYYVLQGQRYVWMETQQAFCQVSLLDHSRTCDDVHCSSSGLSLQDQATRKTIYGPNVISI
PVKSYLQLLADEALNPYYGFQAFSIALWLADHYYWYALCIFLISAISICLALYKTRKQSLTLRDMVKLSV
RVQVCRPGGEEEWVDSSELVPGDCLVLPQEGGVMPCDAALVAGECVVNESSLTGESTPVLKTALPEGPKP
YCPETHRRHTLFCGTLILQARAYVGPRVLAVVTRTGFCTAKGGLVSSILHPRPISFKFYKHSMKFVAALS
VLALLGTVYSIVILYRNRVPVREIVIRALDLVTVVVPPALPAAMTVCTLYAQSRLRAQGIFCIHPLRINL
GGKLRLVCFDKTGTLTEDGLDVMGVVPLKGQVLLPLVPEPRHLPLGPLLRALATCHALSQLHDTPVGDPM
DLKMVESTGWVLEEGPAAGSAPGSQVLVVMRPPPGGPRQQVSCRVTGPWGLGVGLSQPLSSPPTHRKSHQ
CQSASSAASPSPLPCSGWMW

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC023746
ORF Size 1950 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC023746.1
RefSeq Size 4272 bp
RefSeq ORF 1952 bp
Locus ID 74772
Gene Summary ATPase that plays a role in intracellular cation homeostasis and the maintenance of neuronal integrity. Required for a proper lysosomal and mitochondrial maintenance.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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