Hbs1l (BC010251) Mouse Tagged ORF Clone

CAT#: MG210012

  • TrueORF®

Hbs1l (tGFP-tagged) - Mouse Hbs1-like (S. cerevisiae) (cDNA clone MGC:11594 IMAGE:3964560)



  "BC010251" in other vectors (1)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 6,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


HBS1L Antibody - N-terminal region
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 2810035F15Rik; AI326327; eRFS
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG210012 representing BC010251
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCGGCATCGGAACGTCCGGGGATATAACTACGATGAAGATTTTGAAGATGATGATCTGTATGGGC
AGTCTGTGGAGGATGACTACTGTATCTCACCATCAACAGCCGCTCAGTTCATTTACTCACGCCGTGACAA
CCCTGAAGAAGAATATGGCTATGAAGATCTGAGAGAGTCTTCTAATTCTCTTTTGAATCACCAATTAAGT
GAAATTGACCAAGCTCGCCTGTATTCATGCCTTGATCACATGAGAGAGGTACTGGGGGACGCTGTGCCTG
ATGACATACTGACTGAGGCAATTCTGAAACACAAATTTGATGTGCAGAAGGCTTTGTCCGTGGTTCTGGA
ACAAGATGGTGTGCAGCCTTGGAAGGAGAAGAGTGAGCGAGCAGTGTGTGCAGGACAGCCTTCAAAAGGA
AAATCAGTTATATCTCGGTCATCCCAGAGTGAATCTGAAATTGTGCCAAAAGTTGCTAAAATGACTGTAT
CCGGGAAGAAGCAGACCATGGGATTTGAAGTGCCTGGCTTAACTTCTGAGGAAAATGGACTCAGTGTGCG
CGCTCCTCACAAAGGGCCTCCTGGTGATGATGTGAGCGTAGCTTCTCCTAACATTCCTGAGACCGGCACT
CCAAAATCTGCCCTCCCACCTCCTTCCCTTCAAACATCAGAAGAGCTGGGCTCCACTCCGACACCAGTGA
GAAAGTCTGGCAAGCTGAGGCAGCAGATAGACGTGAAAGCGGAATTGGAAAAGCGGCAGGGCGGGAAGCA
GCTCCTCAACTTAGTGGTCATTGGTCATGTTGATGCTGGGAAAAGTACTCTGATGGGCCATATGCTTTAT
CTTCTGGGTAATGTAAACAAAAGAACTATGCATAAGTATGAGCAAGAATCTAAAAAGGCTGGCAAGGCTT
CATTTGCATATGCGTGGGTCTTGGATGAAACTGGAGAAGAAAGGGAAAGGGGAGTAACAATGGACGTCGG
AATGACGAAGTTTGAAACCACAACCAAAGTTATTACCTTAATGGATGCTCCAGGCCATAAGGATTTCATT
CCAAACATGATCACAGGAGCAGCCCAGGCCGATGTCGCTGTCCTGGTCGTTGATGCCAGCAGAGGAGAGT
TTGAAGCTGGATTTGAGACGGGAGGACAGACCCGAGAGCATGGCCTTTTGGTCCGATCTCTTGGAGTGAC
ACAGCTTGCTGTGGCTGTCAATAAGATGGATCAGGTAAATTGGCAACAAGAAAGATTTCAAGAGATTACT
GGAAAACTCGGGCACTTTCTTAAGCAAGCAGGTTTTAAGGAGAGTGATGTAGCTTTTATCCCGACGAGCG
GTCTGAGTGGTGAGAATTTAACCGCAAGGTCACAGTCCAGTGACCTCACGACGTGGTACAAAGGGATGTG
CTTATTGGAGCAGATTGATTCCTTCAAGCCCCCTCAGCGCTCGATTGACAAACCTTTTAGATTATGTGTG
TCTGATGTCTTCAAAGATCAAGGATCTGGCTTTTGTGTGACTGGTAAAATTGAAGCTGGTTATATCCAGA
CTGGTGACCGACTGTTAGCCATGCCACCCAATGAAACCTGTACTGCTAAAGGAATCACTCTGCACGATGA
GCCTGTCGATTGGGCAGCAGCAGGCGATCACGTAAATCTTACCTTGGTTGGGATGGACATCATCAAAATC
AATGTCGGCTGCATATTTTGTGCCCCCAAAGAACCCATTAAGGCTTGCACCCGCTTCAGAGCCCGAATCC
TCGTCTTCAATATTGAAGTTCCGATCACTAAAGGGTTTCCTGTGCTGTTACACTACCAAACCGTCAGTGA
GCCTGCTGTTATTAAGCGATTGATTAGTGTCCTAAACAAAAGTACTGGTGAAGTCACAAAGAAGAAGCCC
AAGTTGCTGACCAAGGGCCAGAACGCCTTGGTAGAGCTGCAGACACAAAGACCAGTGGCTCTTGAGCTCT
ACAAAGACTTCAAGGAGCTGGGGCGGTTTATGCTGCGTTATGGTGGCTCCACAGTAGCTGCCGGTGTTGT
CACTGAGATAAAAGAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG210012 representing BC010251
Red=Cloning site Green=Tags(s)

MARHRNVRGYNYDEDFEDDDLYGQSVEDDYCISPSTAAQFIYSRRDNPEEEYGYEDLRESSNSLLNHQLS
EIDQARLYSCLDHMREVLGDAVPDDILTEAILKHKFDVQKALSVVLEQDGVQPWKEKSERAVCAGQPSKG
KSVISRSSQSESEIVPKVAKMTVSGKKQTMGFEVPGLTSEENGLSVRAPHKGPPGDDVSVASPNIPETGT
PKSALPPPSLQTSEELGSTPTPVRKSGKLRQQIDVKAELEKRQGGKQLLNLVVIGHVDAGKSTLMGHMLY
LLGNVNKRTMHKYEQESKKAGKASFAYAWVLDETGEERERGVTMDVGMTKFETTTKVITLMDAPGHKDFI
PNMITGAAQADVAVLVVDASRGEFEAGFETGGQTREHGLLVRSLGVTQLAVAVNKMDQVNWQQERFQEIT
GKLGHFLKQAGFKESDVAFIPTSGLSGENLTARSQSSDLTTWYKGMCLLEQIDSFKPPQRSIDKPFRLCV
SDVFKDQGSGFCVTGKIEAGYIQTGDRLLAMPPNETCTAKGITLHDEPVDWAAAGDHVNLTLVGMDIIKI
NVGCIFCAPKEPIKACTRFRARILVFNIEVPITKGFPVLLHYQTVSEPAVIKRLISVLNKSTGEVTKKKP
KLLTKGQNALVELQTQRPVALELYKDFKELGRFMLRYGGSTVAAGVVTEIKE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC010251
ORF Size 2048 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC010251, AAH10251
RefSeq Size 2742 bp
RefSeq ORF 2048 bp
Locus ID 56422
Gene Summary Cotranslational quality control factor involved in the No-Go Decay (NGD) pathway. In the presence of ABCE1 and PELO, is required for 48S complex formation from 80S ribosomes and dissociation of vacant 80S ribosomes. Together with PELO and in presence of ABCE1, recognizes stalled ribosomes and promotes dissociation of elongation complexes assembled on non-stop mRNAs; this triggers endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and to degrade damaged mRNAs as part of the No-Go Decay (NGD) pathway.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...