Stim1 (BC021644) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	SIM
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210034 representing BC021644 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGTGTGCGCCCGTCTTGCCCTGTGGCTTCTTTGGGGGCTCCTTCTGCATCAGGGCCAGAGTCTCA GCCATAGTCACAGTGAAAAGAATACAGGAGCTAGCTCCGGGGCGACTTCTGAAGAGTCTACCGAAGCAGA GTTTTGCCGAATTGACAAGCCCCTGTGCCACAGTGAGGATGAGAAGCTCAGCTTTGAGGCCGTCCGAAAC ATCCATAAGCTGATGGATGACGATGCCAATGGTGATGTGGATGTGGAAGAAAGTGATGAGTTCCTAAGGG AAGACCTCAATTACCATGACCCAACAGTGAAACATAGCACCTTCCATGGTGAGGATAAGCTTATCAGCGT GGAGGACCTGTGGAAGGCGTGGAAATCATCAGAAGTGTACAACTGGACTGTGGATGAGGTGATACAGTGG CTCATTACGTATGTGGAGCTGCCACAGTATGAGGAGACCTTCCGGAAGTTGCAGCTTACTGGCCACGCCA TGCCAAGGCTAGCAGTAACCAACACCACCATGACAGGGACTGTACTGAAGATGACAGATCGGAGCCACAG GCAGAAGCTGCAGCTGAAGGCCCTGGACACAGTGCTGTTTGGGCCTCCTCTCTTGACTCGGCATAATCAC CTGAAGGACTTCATGCTGGTGGTGTCTATCGTTATTGGTGTGGGTGGCTGCTGGTTTGCCTATATCCAGA ACCGTTACTCTAAGGAGCACATGAAGAAAATGATGAAGGATCTGGAAGGGTTACACCGGGCTGAGCAGAG TCTGCATGACCTTCAGGAAAGGCTGCACAAGGCCCAGGAGGAGCACCGAACTGTGGAAGTAGAGAAAGTC CACCTGGAGAAGAAGCTGCGAGATGAGATCAACCTTGCCAAGCAGGAAGCTCAGCGGCTGAAGGAGCTGA GGGAGGGTACTGAGAATGAGAGGAGCCGTCAAAAATATGCTGAGGAAGAGCTGGAGCAGGTTCGGGAGGC CTTGAGGAAAGCAGAGAAGGAGCTGGAATCACACAGCTCATGGTATGCTCCTGAGGCCCTGCAGAAGTGG CTGCAGCTGACCCATGAGGTGGAGGTGCAGTACTACAACATCAAGAAGCAAAATGCAGAGAGGCAGCTGC TGGTGGCCAAGGAGGGGGCTGAGAAAATAAAAAAGAAGAGAAACACGCTTTTTGGTACCTTCCATGTGGC CCACAGCTCTTCCCTGGATGATGTGGATCATAAAATCCTAACTGCTAAGCAAGCTCTGAGTGAGGTGACA GCGGCACTGAGGGAGCGCCTGCACCGGTGGCAGCAGATCGAGATCCTCTGCGGTTTCCAGATTGTCAATA ACCCCGGCATCCACTCCTTGGTGGCTGCTCTCAACATCGACCCCAGCTGGATGGGCAGCACCCGCCCTAA CCCCGCCCACTTCATCATGACTGACGATGTGGATGACATGGATGAGGAGATTGTGTCGCCCTTGTCCATG CAGTCCCCCAGCCTGCAGAGCAGTGTCCGGCAGCGCCTGACGGAGCCACAGCTTGGCCTGGGATCTCAGA GGGATTTGACCCATTCCGATTCGGAGTCCTCCCTCCACATGAGTGACCGCCAGCGTGTGGCCCCCAAGCC TCCTCAGATGGGCCGTGCTGCAGATGAAGCTCTCAATGCCATGCCTTCCAATGGCAGCCATCGGCTGATT GAGGGGGTCCATCCAGGATCTCTGGTGGAGAAACTGCCTGACAGCCCTGCTCTGGCCAAGAAGACATTTA TGGCGTTGAACCATGGCCTAGACAAGGCCCACAGCCTGATGGAGCTGAACCCCTCAGTCCCACCTGGTGG CTCCCCACTTTTGGATTCTTCCCATTCTCTTAGCCCCAGTTCCCCAGACCCAGACACACCATCTCCAGTT GGGGACAACCGAGCTCTGCAGGGTAGCCGAAACACACGAATTCCCCACTTGGCTGGCAAGAAGGCAATGG CTGAGGAGGATAATGGTTCCATTGGTGAGGAGACAGACTCCAGTCCAGGCAGGAAGAAGTTTCCTCTCAA AATTTTTAAGAAGCCTCTTAAGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210034 representing BC021644 Red=Cloning site Green=Tags(s) MDVCARLALWLLWGLLLHQGQSLSHSHSEKNTGASSGATSEESTEAEFCRIDKPLCHSEDEKLSFEAVRN IHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVIQW LITYVELPQYEETFRKLQLTGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNH LKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKV HLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKW LQLTHEVEVQYYNIKKQNAERQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVT AALRERLHRWQQIEILCGFQIVNNPGIHSLVAALNIDPSWMGSTRPNPAHFIMTDDVDDMDEEIVSPLSM QSPSLQSSVRQRLTEPQLGLGSQRDLTHSDSESSLHMSDRQRVAPKPPQMGRAADEALNAMPSNGSHRLI EGVHPGSLVEKLPDSPALAKKTFMALNHGLDKAHSLMELNPSVPPGGSPLLDSSHSLSPSSPDPDTPSPV GDNRALQGSRNTRIPHLAGKKAMAEEDNGSIGEETDSSPGRKKFPLKIFKKPLKK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC021644
ORF Size	2057 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC021644, AAH21644
RefSeq Size	3619 bp
RefSeq ORF	2057 bp
Locus ID	20866
Gene Summary	Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit ORAI1. Involved in enamel formation. Activated following interaction with STIMATE, leading to promote STIM1 conformational switch.[UniProtKB/Swiss-Prot Function]

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