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Kif3a (NM_008443) Mouse Tagged ORF Clone
CAT＃: MG210130
- TrueORF®
Kif3a (tGFP-tagged) - Mouse kinesin family member 3A (Kif3a)
ORF Plasmid: DDK
"NM_008443" in other vectors (4)
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CNY 7,700.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Kif3; Kifl; Kns3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210130 representing NM_008443
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGATCAATAAGTCGGAGAAGCCGGAAAGCTGCGATAATGTGAAGGTGGTGGTTAGGTGCCGGCCTC TCAATGAGAGAGAGAAGTCAATGTGCTATAGACAGGCCGTCAGCGTGGATGAAATGAGGGGAACTATCAC CGTCCATAAGACCGATTCCTCCAACGAACCTCCAAAGACATTTACTTTCGATACTGTTTTTGGACCAGAA AGTAAACAACTTGATGTGTATAACTTAACTGCAAGACCAATTATTGATTCTGTTCTGGAAGGCTACAATG GGACCATCTTTGCATATGGACAGACTGGGACAGGCAAGACTTTCACCATGGAAGGCGTTCGAGCAGTACC GGGGCTCAGAGGGGTCATCCCCAACTCATTTGCTCACATATTTGGTCACATCGCAAAAGCAGAGGGCGAC ACAAGGTTTTTGGTTCGAGTGTCATACTTGGAGATATATAATGAAGAAGTTCGAGACCTTTTGGGTAAAG ATCAGACCCAGAGGTTAGAGGTTAAAGAAAGGCCTGATGTGGGAGTATATATCAAAGACTTATCAGCATA TGTAGTCAACAACGCTGACGACATGGATAGAATCATGACTCTAGGCCACAAAAACCGTTCTGTCGGTGCG ACTAATATGAACGAGCACAGCTCCCGATCCCACGCCATCTTTACAATTACTATAGAGTGCAGTGAGAAAG GCGTGGACGGGAACATGCACGTCAGGATGGGCAAGCTCCACCTTGTGGATCTGGCTGGGTCAGAAAGACA AGCAAAAACTGGAGCCACAGGACAGCGCCTCAAGGAAGCTACCAAAATCAACCTTTCATTGTCTACCCTG GGCAACGTTATTTCTGCCTTGGTGGATGGAAAAAGCACTCACGTGCCGTATCGGAACTCCAAACTGACCC GTCTCCTGCAGGACTCCTTAGGAGGGAACTCAAAAACCATGATGTGTGCAAATATTGGGCCAGCAGATTA TAATTATGATGAAACTATCAGCACCTTGCGGTATGCCAACCGCGCCAAGAACATTAAAAACAAAGCGAGA ATCAATGAGGACCCAAAGGATGCTCTGCTCCGCCAGTTTCAGAAAGAAATCGAAGAGCTGAAGAAAAAGC TTGAGGAAGGGGAAGAAGTGTCAGGCTCTGACATCAGTGGGTCAGAGGAGGATGACGAGGAGGGTGAACT TGGAGAAGATGGAGAAAAAAAGAAAAAGAGAAGGGACCAAGCAGGTAAAAAGAAAGTTTCCCCAGATAAG ATGGTGGAAATGCAAGCGAAAATTGACGAGGAGAGAAAAGCACTGGAAACCAAACTTGACATGGAAGAAG AAGAAAGAAACAAGGCTAGAGCTGAACTAGAGAGACGGGAGAAGGACCTTCTGAAAGCCCAACAAGAGCA TCAGTCTTTGCTGGAAAAGCTGTCTGCTTTGGAAAAGAAGGTCATTGTCGGCGGCGTGGACCTGCTGGCC AAGGCTGAGGAGCAAGAGAAGCTTCTGGAGGAGTCCAACATGGAGCTGGAGGAGAGGAGGAGACGAGCTG AGCAGCTTCGGAAAGAGCTGGAGGAAAAGGAGCAAGAACGCTTGGACATCGAGGAAAAATATACCAGTCT GCAAGAGGAGGCACAAGGAAAGACCAAGAAATTAAAGAAAGTCTGGACCATGCTGATGGCTGCAAAGTCA GAGATGGCTGATCTCCAGCAGGAGCATCAGAGGGAGATCGAAGGCCTCCTGGAGAACATCCGGCAGCTCA GCCGGGAGCTCCGTCTTCAGATGCTCATCATTGATAACTTCATACCTCAGGATTACCAGGAAATGATTGA AAACTACGTCCACTGGAATGAAGACATAGGAGAATGGCAGCTAAAATGTGTGGCCTACACAGGAAATAAC ATGAGGAAACAAACTCCAGTCCCTGACAAGAAGGAGAGAGACCCATTTGAAGTCGACCTTTCCCACGTGT ACCTCGCCTATACAGAGGAGAGTCTGCGTCAGTCTCTGATGAAGTTAGAAAGGCCACGGACCTCCAAGGG GAAAGCAAGGCCAAAGATGGGGCGAAGAAAGCGTTCTGCAAAGCCTGAGACCGTAATTGATTCTTTACTT CAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210130 representing NM_008443
Red=Cloning site Green=Tags(s) MPINKSEKPESCDNVKVVVRCRPLNEREKSMCYRQAVSVDEMRGTITVHKTDSSNEPPKTFTFDTVFGPE SKQLDVYNLTARPIIDSVLEGYNGTIFAYGQTGTGKTFTMEGVRAVPGLRGVIPNSFAHIFGHIAKAEGD TRFLVRVSYLEIYNEEVRDLLGKDQTQRLEVKERPDVGVYIKDLSAYVVNNADDMDRIMTLGHKNRSVGA TNMNEHSSRSHAIFTITIECSEKGVDGNMHVRMGKLHLVDLAGSERQAKTGATGQRLKEATKINLSLSTL GNVISALVDGKSTHVPYRNSKLTRLLQDSLGGNSKTMMCANIGPADYNYDETISTLRYANRAKNIKNKAR INEDPKDALLRQFQKEIEELKKKLEEGEEVSGSDISGSEEDDEEGELGEDGEKKKKRRDQAGKKKVSPDK MVEMQAKIDEERKALETKLDMEEEERNKARAELERREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLA KAEEQEKLLEESNMELEERRRRAEQLRKELEEKEQERLDIEEKYTSLQEEAQGKTKKLKKVWTMLMAAKS EMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPQDYQEMIENYVHWNEDIGEWQLKCVAYTGNN MRKQTPVPDKKERDPFEVDLSHVYLAYTEESLRQSLMKLERPRTSKGKARPKMGRRKRSAKPETVIDSLL Q TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008443 |
| ORF Size | 2103 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008443.4, NP_032469.2 |
| RefSeq Size | 5438 bp |
| RefSeq ORF | 2106 bp |
| Locus ID | 16568 |
| UniProt ID | P28741 |
| Gene Summary | Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation (PubMed:21670265). Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitement of DCTN1 to the centriole. Also required for ciliary basal feet formation and microtubule anchoring to mother centriole (PubMed:23386061).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220570 | Kif3a (untagged) - Mouse kinesin family member 3A (Kif3a), (10ug) |
CNY 7,712.00 |
|
| MR210130 | Kif3a (Myc-DDK-tagged) - Mouse kinesin family member 3A (Kif3a) |
CNY 7,030.00 |
|
| MR210130L3 | Lenti ORF clone of Kif3a (Myc-DDK-tagged) - Mouse kinesin family member 3A (Kif3a) |
CNY 7,536.00 |
|
| MR210130L4 | Lenti ORF clone of Kif3a (mGFP-tagged) - Mouse kinesin family member 3A (Kif3a) |
CNY 8,930.00 |
