Spire2 (NM_172287) Mouse Tagged ORF Clone
CAT#: MG210260
- TrueORF®
Spire2 (tGFP-tagged) - Mouse spire homolog 2 (Drosophila) (Spire2)
ORF Plasmid: DDK
"NM_172287" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 6,940.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | BC026502; Spir-2; Spir2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG210260 representing NM_172287
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGGGCGGGCGGCGGCGGCGCAGCAGCTCCCGAACGCGCGGGGGGCGCGGCGCGGCCCGAGCCGT GGGAGCTGTCCCTGGAGGAAGTGCTGAAGGTGTACGAGCAGCCCATCAACGAGGAGCAGGCCTGGGCGGT GTGCTTCCAGGGCTGCCGCGGGCTGCGGGGCGAGCCGGGCGGCGTGCGGCGCATCCGGGACACGGCAGAC ATCCTCCTGCGTAGGGACGGTTCGGTTGGCGCGCGGCTGGAGCCCGAGCCTACAACCATGGTGGTACCAC CAGCCAGCTCAGAAGCCCAGATGGTGCAGTCACTGGGCTTTGCCATTTACCGTGCGCTGGACTGGGGGTT AGATGAGAACGAGGAGAGAGAGCTCAGCCCGCAGCTGGAAAGGCTCATCGACCTCATGGCCAACAGCGAC TGCGAAGACAGCAGCTGCGGGGCAGCGGATGAGGGCTACGTGGGTCCAGAAGAGGAGGAGGAGGCTGAGG GCGGCCCCCGAGCTGTGCGCACCTTTGCCCAGGCTATGCGGCTGTGTGCTTTGCGCCTGACCGACCCCCA CGGCGCGCAGGCCCACTACCAGGCAGTGTGCCGAGCTCTCTTTGTGGAGACACTGGAGCTTCGTGCCTTC CTGGCCCGGGTGAGGGAGGCCAAAGAGATGCTGAAGAAACTGGGGGAGGAGGAGCCACGGGAGAAGCCAC TGGCCGAGCTCGACCACCTGGGACACACAGACTGGGCCCGGCTGTGGGTCCAGCTCATGCGGGAGCTCCG TCACGGGGTAAAGCTGAAGAAGGTGCAGGAGAAGGAGTTCAACCCACTGCCCACCGAGTTCCAGCTCACC CCCTTCGAGATGCTGATGCAGGATATCCGTGCCAGGAACTATAAGCTGCGCAAGGTCATGGTCGATGGGG ACATCCCTCCCAGGGTGAAGAAGGACGCACATGAACTTATCCTGGACTTCATTCGTTCTCGGCCTCCACT GAAGCAGGTCTCAGAGAGACAACTTCGCCCTGTACCACAGAAGCAAAGAACACTGCACGAGAAGATCCTG GAGGAAATCAAGCAGGAGCGGAGGCTTCGTCCAGTCGGGGCCCAGCACTTGGGAGGCCGTGGGTTTGGTT CCCTGCCCTGCATCCTCAATGCCTGCTCTGGGGACATCAAGTCCACTTCCTGCATCAACCTGTCTGTCAC CGATACTGGGAGCGGGAGTCAGCGCCCACGGCCCCGGGTCCTGCTCAAAGCCCCCACCCTTGCAGAGATG GAAGAAATGAACACGTCTGAGGAAGAAGAGTCCCCATGTGGGGAGGTGGCACTGAAGCGGGACCGCTCTT TTTCGGAACACGACCTGGCCCAGCTTCGGAGCGAGATGGCATCTGGCCTTCAGTCAGCCGCTCAGCCCCC AGGAGGGACGGAGCCACCTCGGGCCAGAGCAGGTAGCATGCACTCCTGGAGGCCCAGCAGCCGAGACCAG GGTTTCTGTCCTGTGAGCGGCCAGTCCCAGCCTCTCCCCAGCTCTGCCTTGCCCAGTAGCTTGAGCTCAG TGGATGGACCTGAGGCGGCTTCACCAGATACCAGACACCTGTGGCTGGAGTTCAGCCACCCTGTGGAGAG CCTGGCTCTGACTGTGGAAGAGGTGGTGGACGTGCGCAGGGTGCTGGTGAAGGCGGAGATGGAGAGGTTT CTGCAGGACAAGGAGCTCTTCAGCAGCCTGAAGAGGGGGAAGATTTGCTGTTGCTGCCGAGCCAAGTTTC CCCTGTTTTCCTGGCCACCCACCTGTCTCTTCTGCAAGAGAGCTGTGTGCACTTCCTGTAGCGTAAAGAT GAAGATGCCTTCCAAGAAGTATGGACACATCCCTGTCTACACACTTGGCTTTGAGAGTCTTCAGAGGGTG CCAACTACCAAAGCCACGCCAACACTGAGGAGAGATGCCTTCCAATCCCTGCAGGGACCAAAGTGGCGGA GCGTGGAGGAGGAGTTCCCCCACATCTATGCCCACGGCTGTGTCCTAAAGGATGTCTGCAGTGACTGCAC CAGCTTTGTGGCTGATGTCGTGTGCTCCAGCCGCAAGAGCGTGGATGTCCTCAATGCCACTCCACGACGA AGCCGTCAGACCCAGTCCCTCTATATCCCCAACACCAGGACTCTCAACTTCCAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210260 representing NM_172287
Red=Cloning site Green=Tags(s) MARAGGGGAAAPERAGGAARPEPWELSLEEVLKVYEQPINEEQAWAVCFQGCRGLRGEPGGVRRIRDTAD ILLRRDGSVGARLEPEPTTMVVPPASSEAQMVQSLGFAIYRALDWGLDENEERELSPQLERLIDLMANSD CEDSSCGAADEGYVGPEEEEEAEGGPRAVRTFAQAMRLCALRLTDPHGAQAHYQAVCRALFVETLELRAF LARVREAKEMLKKLGEEEPREKPLAELDHLGHTDWARLWVQLMRELRHGVKLKKVQEKEFNPLPTEFQLT PFEMLMQDIRARNYKLRKVMVDGDIPPRVKKDAHELILDFIRSRPPLKQVSERQLRPVPQKQRTLHEKIL EEIKQERRLRPVGAQHLGGRGFGSLPCILNACSGDIKSTSCINLSVTDTGSGSQRPRPRVLLKAPTLAEM EEMNTSEEEESPCGEVALKRDRSFSEHDLAQLRSEMASGLQSAAQPPGGTEPPRARAGSMHSWRPSSRDQ GFCPVSGQSQPLPSSALPSSLSSVDGPEAASPDTRHLWLEFSHPVESLALTVEEVVDVRRVLVKAEMERF LQDKELFSSLKRGKICCCCRAKFPLFSWPPTCLFCKRAVCTSCSVKMKMPSKKYGHIPVYTLGFESLQRV PTTKATPTLRRDAFQSLQGPKWRSVEEEFPHIYAHGCVLKDVCSDCTSFVADVVCSSRKSVDVLNATPRR SRQTQSLYIPNTRTLNFQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_172287 |
ORF Size | 2154 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_172287.2, NP_758491.1 |
RefSeq Size | 2399 bp |
RefSeq ORF | 2157 bp |
Locus ID | 234857 |
UniProt ID | Q8K1S6 |
Gene Summary | Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament (PubMed:21620703, PubMed:21983562). Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport (PubMed:21983562). Required for asymmetric spindle positioning and asymmetric cell division during oocyte meiosis (PubMed:21620703). Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis (PubMed:21620703). Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203353 | Spire2 (untagged) - Mouse spire homolog 2 (Drosophila) (Spire2), (10ug) |
CNY 5,264.00 |
|
MR210260 | Spire2 (Myc-DDK-tagged) - Mouse spire homolog 2 (Drosophila) (Spire2) |
CNY 5,264.00 |
|
MR210260L3 | Lenti ORF clone of Spire2 (Myc-DDK-tagged) - Mouse spire homolog 2 (Drosophila) (Spire2) |
CNY 8,170.00 |
|
MR210260L4 | Lenti ORF clone of Spire2 (mGFP-tagged) - Mouse spire homolog 2 (Drosophila) (Spire2) |
CNY 8,170.00 |