Hsp90aa1 (BC049124) Mouse Tagged ORF Clone

CAT#: MG210348

  • TrueORF®

Hsp90aa1 (tGFP-tagged) - Mouse heat shock protein 90kDa alpha (cytosolic), class A member 1 (cDNA clone MGC:61211 IMAGE:6816250)



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Mouse Protein / Lysate Request

CNY 8,080.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Hsp90aa1 Antibody - C-terminal region
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 86kDa, 89kDa
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG210348 representing BC049124
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTGAGGAAACCCAGACCCAAGACCAACCAATGGAGGAGGAGGAGGTCGAGACCTTTGCCTTTCAGG
CAGAAATTGCCCAGTTAATGTCCTTGATCATCAATACCTTCTACTCGAACAAAGAGATCTTTCTGAGGGA
GCTCATCTCCAATTCATCGGACGCTCTGGATAAAATCCGTTACGAGAGCCTGACGGACCCCAGTAAACTG
GACTCGGGGAAGGAGCTGCACATCAATCTCATTCCCAGCAAACAGGACCGAACCCTGACCATTGTGGATA
CCGGGATTGGAATGACCAAGGCCGACTTGATCAATAACCTTGGCACCATTGCCAAGTCGGGCACCAAAGC
CTTCATGGAGGCTTTGCAGGCTGGTGCAGATATCTCTATGATTGGCCAGTTTGGTGTTGGTTTTTACTCT
GCCTATTTGGTTGCTGAGAAAGTGACTGTCATCACGAAGCATAACGACGATGAGCAGTATGCCTGGGAGT
CCTCAGCTGGGGGATCCTTCACAGTGAGGACTGACACAGGTGAACCAATGGGTCGTGGAACAAAGGTTAT
CTTGCATCTGAAAGAAGACCAAACAGAGTATTTGGAGGAAAGGAGAATAAAGGAGATCGTGAAGAAGCAT
TCTCAGTTCATTGGCTATCCCATTACTCTCTTTGTGGAGAAGGAACGAGATAAGGAAGTCAGTGATGATG
AGGCTGAAGAAAAGGAAGAGAAAGAGGAAGAGAAAGAAAAAGAAGAAAAGGAGTCTGATGATAAACCTGA
AATAGAAGATGTTGGCTCTGATGAAGAAGAGGAGGAGAAGAAGGATGGTGACAAGAAGAAAAAGAAGAAG
ATAAAGGAAAAGTACATTGATCAAGAAGAACTCAACAAAACAAAGCCGATTTGGACGAGAAATCCTGATG
ACATCACTAATGAGGAATATGGAGAGTTCTACAAGAGCTTAACTAACGATTGGGAAGAACACTTGGCAGT
AAAGCATTTTTCTGTTGAAGGACAATTAGAATTCCGGGCCCTTCTTTTTGTCCCAAGACGCGCTCCTTTT
GATCTGTTTGAAAACAGAAAGAAAAAGAACAACATCAAGTTGTATGTTCGCAGAGTTTTTATCATGGATA
ACTGTGAGGAATTAATCCCTGAGTATCTGAATTTCATTAGAGGGGTAGTGGATTCTGAGGATCTCCCTCT
AAATATTTCCCGTGAAATGCTGCAACAAAGTAAAATTCTGAAAGTTATCAGAAAGAATTTGGTCAAGAAA
TGCTTAGAACTATTTACTGAACTAGCAGAAGATAAAGAGAACTACAAAAAGTTTTATGAGCAGTTCTCAA
AAAATATAAAGCTTGGAATTCACGAGGACTCTCAGAATCGGAAGAAGCTTTCAGAGCTGTTGCGGTACTA
CACATCTGCTTCTGGGGACGAGATGGTTTCTCTGAAGGACTACTGTACCAGAATGAAGGAAAACCAGAAG
CACATCTATTTTATCACAGGTGAGACCAAGGACCAGGTTGCTAACTCCGCCTTTGTGGAACGTCTCCGAA
AGCATGGCTTAGAAGTAATTTATATGATTGAGCCCATTGATGAGTATTGTGTGCAACAGCTGAAGGAATT
TGAGGGCAAGACCTTGGTGTCTGTTACCAAAGAAGGACTGGAACTTCCAGAAGATGAAGAGGAAAAGAAG
AAACAGGAAGAGAAAAAGACAAAATTTGAGAACCTCTGCAAAATTATGAAAGATATTTTGGAGAAGAAGG
TTGAAAAGGTGGTTGTGTCAAACCGACTGGTGACATCCCCGTGCTGTATTGTCACAAGCACATATGGGTG
GACAGCAAACATGGAGAGAATCATGAAAGCTCAAGCCCTCAGAGACAACTCAACAATGGGTTACATGGCA
GCAAAGAAACACCTGGAGATAAATCCTGATCACTCCATTATTGAAACCTTAAGGCAAAAGGCAGAGGCTG
ACAAGAATGACAAATCTGTGAAGGATCTGGTCATCTTGCTGTATGAAACTGCACTCCTATCTTCTGGCTT
CAGTCTGGAAGATCCCCAGACCCATGCTAACAGGATCTACAGGATGATCAAGCTTGGTCTAGGTATTGAT
GAGGATGATCCTACTGTGGATGACACCAGTGCTGCTGTAACTGAAGAAATGCCTCCCCTGGAAGGAGATG
ACGACACATCACGCATGGAAGAAGTAGAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG210348 representing BC049124
Red=Cloning site Green=Tags(s)

MPEETQTQDQPMEEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESLTDPSKL
DSGKELHINLIPSKQDRTLTIVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYS
AYLVAEKVTVITKHNDDEQYAWESSAGGSFTVRTDTGEPMGRGTKVILHLKEDQTEYLEERRIKEIVKKH
SQFIGYPITLFVEKERDKEVSDDEAEEKEEKEEEKEKEEKESDDKPEIEDVGSDEEEEEKKDGDKKKKKK
IKEKYIDQEELNKTKPIWTRNPDDITNEEYGEFYKSLTNDWEEHLAVKHFSVEGQLEFRALLFVPRRAPF
DLFENRKKKNNIKLYVRRVFIMDNCEELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNLVKK
CLELFTELAEDKENYKKFYEQFSKNIKLGIHEDSQNRKKLSELLRYYTSASGDEMVSLKDYCTRMKENQK
HIYFITGETKDQVANSAFVERLRKHGLEVIYMIEPIDEYCVQQLKEFEGKTLVSVTKEGLELPEDEEEKK
KQEEKKTKFENLCKIMKDILEKKVEKVVVSNRLVTSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMA
AKKHLEINPDHSIIETLRQKAEADKNDKSVKDLVILLYETALLSSGFSLEDPQTHANRIYRMIKLGLGID
EDDPTVDDTSAAVTEEMPPLEGDDDTSRMEEVD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC049124
ORF Size 2201 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC049124, AAH49124
RefSeq Size 2869 bp
RefSeq ORF 2201 bp
Locus ID 15519
Gene Summary Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle. Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. In the first place, they alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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