Hsp90aa1 (BC049124) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	86kDa, 89kDa
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210348 representing BC049124 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGAGGAAACCCAGACCCAAGACCAACCAATGGAGGAGGAGGAGGTCGAGACCTTTGCCTTTCAGG CAGAAATTGCCCAGTTAATGTCCTTGATCATCAATACCTTCTACTCGAACAAAGAGATCTTTCTGAGGGA GCTCATCTCCAATTCATCGGACGCTCTGGATAAAATCCGTTACGAGAGCCTGACGGACCCCAGTAAACTG GACTCGGGGAAGGAGCTGCACATCAATCTCATTCCCAGCAAACAGGACCGAACCCTGACCATTGTGGATA CCGGGATTGGAATGACCAAGGCCGACTTGATCAATAACCTTGGCACCATTGCCAAGTCGGGCACCAAAGC CTTCATGGAGGCTTTGCAGGCTGGTGCAGATATCTCTATGATTGGCCAGTTTGGTGTTGGTTTTTACTCT GCCTATTTGGTTGCTGAGAAAGTGACTGTCATCACGAAGCATAACGACGATGAGCAGTATGCCTGGGAGT CCTCAGCTGGGGGATCCTTCACAGTGAGGACTGACACAGGTGAACCAATGGGTCGTGGAACAAAGGTTAT CTTGCATCTGAAAGAAGACCAAACAGAGTATTTGGAGGAAAGGAGAATAAAGGAGATCGTGAAGAAGCAT TCTCAGTTCATTGGCTATCCCATTACTCTCTTTGTGGAGAAGGAACGAGATAAGGAAGTCAGTGATGATG AGGCTGAAGAAAAGGAAGAGAAAGAGGAAGAGAAAGAAAAAGAAGAAAAGGAGTCTGATGATAAACCTGA AATAGAAGATGTTGGCTCTGATGAAGAAGAGGAGGAGAAGAAGGATGGTGACAAGAAGAAAAAGAAGAAG ATAAAGGAAAAGTACATTGATCAAGAAGAACTCAACAAAACAAAGCCGATTTGGACGAGAAATCCTGATG ACATCACTAATGAGGAATATGGAGAGTTCTACAAGAGCTTAACTAACGATTGGGAAGAACACTTGGCAGT AAAGCATTTTTCTGTTGAAGGACAATTAGAATTCCGGGCCCTTCTTTTTGTCCCAAGACGCGCTCCTTTT GATCTGTTTGAAAACAGAAAGAAAAAGAACAACATCAAGTTGTATGTTCGCAGAGTTTTTATCATGGATA ACTGTGAGGAATTAATCCCTGAGTATCTGAATTTCATTAGAGGGGTAGTGGATTCTGAGGATCTCCCTCT AAATATTTCCCGTGAAATGCTGCAACAAAGTAAAATTCTGAAAGTTATCAGAAAGAATTTGGTCAAGAAA TGCTTAGAACTATTTACTGAACTAGCAGAAGATAAAGAGAACTACAAAAAGTTTTATGAGCAGTTCTCAA AAAATATAAAGCTTGGAATTCACGAGGACTCTCAGAATCGGAAGAAGCTTTCAGAGCTGTTGCGGTACTA CACATCTGCTTCTGGGGACGAGATGGTTTCTCTGAAGGACTACTGTACCAGAATGAAGGAAAACCAGAAG CACATCTATTTTATCACAGGTGAGACCAAGGACCAGGTTGCTAACTCCGCCTTTGTGGAACGTCTCCGAA AGCATGGCTTAGAAGTAATTTATATGATTGAGCCCATTGATGAGTATTGTGTGCAACAGCTGAAGGAATT TGAGGGCAAGACCTTGGTGTCTGTTACCAAAGAAGGACTGGAACTTCCAGAAGATGAAGAGGAAAAGAAG AAACAGGAAGAGAAAAAGACAAAATTTGAGAACCTCTGCAAAATTATGAAAGATATTTTGGAGAAGAAGG TTGAAAAGGTGGTTGTGTCAAACCGACTGGTGACATCCCCGTGCTGTATTGTCACAAGCACATATGGGTG GACAGCAAACATGGAGAGAATCATGAAAGCTCAAGCCCTCAGAGACAACTCAACAATGGGTTACATGGCA GCAAAGAAACACCTGGAGATAAATCCTGATCACTCCATTATTGAAACCTTAAGGCAAAAGGCAGAGGCTG ACAAGAATGACAAATCTGTGAAGGATCTGGTCATCTTGCTGTATGAAACTGCACTCCTATCTTCTGGCTT CAGTCTGGAAGATCCCCAGACCCATGCTAACAGGATCTACAGGATGATCAAGCTTGGTCTAGGTATTGAT GAGGATGATCCTACTGTGGATGACACCAGTGCTGCTGTAACTGAAGAAATGCCTCCCCTGGAAGGAGATG ACGACACATCACGCATGGAAGAAGTAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210348 representing BC049124 Red=Cloning site Green=Tags(s) MPEETQTQDQPMEEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESLTDPSKL DSGKELHINLIPSKQDRTLTIVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYS AYLVAEKVTVITKHNDDEQYAWESSAGGSFTVRTDTGEPMGRGTKVILHLKEDQTEYLEERRIKEIVKKH SQFIGYPITLFVEKERDKEVSDDEAEEKEEKEEEKEKEEKESDDKPEIEDVGSDEEEEEKKDGDKKKKKK IKEKYIDQEELNKTKPIWTRNPDDITNEEYGEFYKSLTNDWEEHLAVKHFSVEGQLEFRALLFVPRRAPF DLFENRKKKNNIKLYVRRVFIMDNCEELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNLVKK CLELFTELAEDKENYKKFYEQFSKNIKLGIHEDSQNRKKLSELLRYYTSASGDEMVSLKDYCTRMKENQK HIYFITGETKDQVANSAFVERLRKHGLEVIYMIEPIDEYCVQQLKEFEGKTLVSVTKEGLELPEDEEEKK KQEEKKTKFENLCKIMKDILEKKVEKVVVSNRLVTSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMA AKKHLEINPDHSIIETLRQKAEADKNDKSVKDLVILLYETALLSSGFSLEDPQTHANRIYRMIKLGLGID EDDPTVDDTSAAVTEEMPPLEGDDDTSRMEEVD TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC049124
ORF Size	2201 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC049124, AAH49124
RefSeq Size	2869 bp
RefSeq ORF	2201 bp
Locus ID	15519
Gene Summary	Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle. Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. In the first place, they alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation.[UniProtKB/Swiss-Prot Function]

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