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Nsf (NM_008740) Mouse Tagged ORF Clone
CAT＃: MG210421
- TrueORF®
Nsf (tGFP-tagged) - Mouse N-ethylmaleimide sensitive fusion protein (Nsf)
ORF Plasmid: DDK
"NM_008740" in other vectors (3)
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CNY 7,130.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI316878; AU020090; AU067812; SKD2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210421 representing NM_008740
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCCGGACTATGCAAGCTGCGAGATGCCCTACTGATGAATTATCTTTAAGCAACTGTGCGGTTG TGAACGAAAAAGATTTCCAGTCTGGCCAGCATGTGATGGTGAGGACGTCCCCCAATCACAAGTACATATT TACGCTGAGGACCCATCCGTCAGTGGTTCCAGGATGCATCGCGTTCAGCTTGCCTCAGCGAAAATGGGCT GGACTTTCTATTGGACAGGACATAGAAGTTGCCTTGTATTCGTTTGACAAAGCCAAGCAGTGCATCGGCA CAATGACCATCGAGATCGACTTCCTGCAGAAGAAGAACATCGACTCCAACCCTTACGACACCGACAAGAT GGCCGCCGAGTTCATCCAGCAGTTCAACAACCAGGCCTTCTCAGTGGGGCAGCAGCTTGTCTTTAGCTTC AATGATAAGCTCTTTGGATTACTGGTGAAGGACATTGAAGCCATGGATCCCAGCATCCTGAAGGGAGAGC CTGCATCAGGCAAAAGGCAGAAGATTGAGGTAGGCCTGGTTGTTGGAAACAGTCAAGTTGCATTTGAAAA AGCAGAAAATTCGTCGCTCAATCTTATCGGCAAAGCTAAAACCAAGGAAAATCGCCAGTCCATCATCAAT CCCGACTGGAACTTTGAGAAAATGGGGATCGGCGGTCTGGATAAGGAGTTCTCGGACATTTTCCGAAGAG CATTTGCTTCTCGGGTGTTCCCCCCGGAGATCGTGGAACAGATGGGTTGCAAACATGTTAAAGGCATCCT GTTATACGGACCCCCTGGTTGTGGTAAAACTCTCTTGGCTCGACAGATTGGCAAGATGCTGAATGCGAGA GAGCCCAAGGTGGTCAATGGGCCAGAAATCCTTAACAAGTATGTGGGAGAATCAGAGGCTAACATTCGTA AACTCTTTGCTGATGCCGAAGAGGAGCAAAGGAGGCTTGGTGCTAACAGTGGCTTGCACATCATCATCTT TGATGAAATCGATGCCATCTGCAAGCAGAGAGGGAGCATGGCCGGCAGCACTGGAGTGCATGACACCGTG GTCAACCAGCTGCTGTCCAAGATCGATGGCGTTGAGCAGCTGAACAACATCCTCGTTATCGGAATGACCA ATAGACCAGATTTGATAGACGAAGCTCTCCTTCGACCCGGAAGACTGGAAGTTAAAATGGAGATAGGCTT GCCAGATGAGAAGGGTCGACTCCAGATCCTTCATATCCACACAGCAAGGATGAGAGGGCACCAGTTACTG TCTGCAGATGTGGACATCAAGGAGCTGGCTGTGGAGACTAAGAATTTCAGTGGCGCTGAGCTGGAGGGTC TGGTGCGAGCAGCGCAGTCCACAGCCATGAACAGACATATCAAGGCCAGCACCAAAGTAGAAGTAGACAT GGAGAAGGCAGAGAGCCTGCAGGTGACAAGAGGGGACTTCCTTGCTTCCCTGGAGAACGATATCAAACCA GCATTTGGCACCAACCAAGAGGATTATGCAAGTTACATTATGAATGGGATCATCAAGTGGGGTGACCCCG TGACCCGAGTCCTAGATGACGGAGAGCTGCTGGTCCAGCAAACGAAAAACAGTGACCGGACACCACTCGT TAGTGTCCTCCTAGAAGGGCCTCCTCACAGCGGGAAGACTGCCCTGGCTGCAAAGATTGCCGAGGAATCC AACTTCCCCTTCATCAAGATCTGCTCACCTGATAAGATGATTGGCTTCTCCGAGACAGCCAAGTGTCAGG CCATGAAGAAGATCTTTGATGATGCTTACAAATCTCAGCTCAGCTGTGTGGTCGTGGATGACATTGAGAG GCTGCTCGATTATGTCCCCATTGGTCCCCGGTTCTCCAACCTGGTATTGCAGGCTCTTCTTGTGTTACTG AAAAAGGCGCCTCCTCAGGGCCGCAAGCTTCTTATCATCGGGACCACCAGCCGCAAAGATGTCCTGCAGG AAATGGAGATGCTGAACGCCTTCAGCACCACCATCCACGTGCCCAACATTGCCACAGGAGAGCAGCTGCT GGAAGCTCTGGAGCTTTTGGGCAACTTCAAAGATAAGGAACGCACCACAATTGCTCAGCAAGTCAAAGGG AAGAAAGTCTGGATAGGAATCAAGAAGTTACTAATGTTGATTGAGATGTCCCTGCAGATGGATCCTGAGT ACCGTGTGAGGAAATTCTTGGCCCTCATGAGAGAAGAAGGCGCTAGTCCCCTGGACTTTGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210421 representing NM_008740
Red=Cloning site Green=Tags(s) MAGRTMQAARCPTDELSLSNCAVVNEKDFQSGQHVMVRTSPNHKYIFTLRTHPSVVPGCIAFSLPQRKWA GLSIGQDIEVALYSFDKAKQCIGTMTIEIDFLQKKNIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSF NDKLFGLLVKDIEAMDPSILKGEPASGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIIN PDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAR EPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTV VNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLL SADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKP AFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEES NFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLL KKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFSTTIHVPNIATGEQLLEALELLGNFKDKERTTIAQQVKG KKVWIGIKKLLMLIEMSLQMDPEYRVRKFLALMREEGASPLDFD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008740 |
| ORF Size | 2232 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008740.4 |
| RefSeq Size | 3787 bp |
| RefSeq ORF | 2235 bp |
| Locus ID | 18195 |
| UniProt ID | P46460 |
| Gene Summary | Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack GRIA2 leads to influence GRIA2 membrane cycling (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR210421 | Nsf (Myc-DDK-tagged) - Mouse N-ethylmaleimide sensitive fusion protein (Nsf) |
CNY 5,456.00 |
|
| MR210421L3 | Lenti ORF clone of Nsf (Myc-DDK-tagged) - Mouse N-ethylmaleimide sensitive fusion protein (Nsf) |
CNY 8,360.00 |
|
| MR210421L4 | Lenti ORF clone of Nsf (mGFP-tagged) - Mouse N-ethylmaleimide sensitive fusion protein (Nsf) |
CNY 8,360.00 |
